Profiling indigenous lead-reducing bacteria from Tempe Lake, South Sulawesi, Indonesia as bioremediation agents

Abstract. Yani A, Amin M, Rohman F, Suarsini E, Putra WE. 2020. Profiling indigenous lead-reducing bacteria from Tempe Lake, South Sulawesi, Indonesia as bioremediation agents. Biodiversitas 21: 4778-4786. The pollution of heavy metals by anthropogenic activities in freshwater, especially in lakes,...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	AHMAD YANI, MOHAMAD AMIN, Fatchur Rohman, ENDANG SUARSINI, WIRA EKA PUTRA
Formato:	article
Lenguaje:	EN
Publicado:	MBI & UNS Solo 2020
Materias:	bioremediation, comamonas testosteroni, indigenous bacteria, lake tempe, lead-reduction Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/385fb98f93864584839fb6696af0f019
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:385fb98f93864584839fb6696af0f019
record_format	dspace
spelling	oai:doaj.org-article:385fb98f93864584839fb6696af0f0192021-11-22T00:51:08ZProfiling indigenous lead-reducing bacteria from Tempe Lake, South Sulawesi, Indonesia as bioremediation agents1412-033X2085-472210.13057/biodiv/d211043https://doaj.org/article/385fb98f93864584839fb6696af0f0192020-09-01T00:00:00Zhttps://smujo.id/biodiv/article/view/6373https://doaj.org/toc/1412-033Xhttps://doaj.org/toc/2085-4722Abstract. Yani A, Amin M, Rohman F, Suarsini E, Putra WE. 2020. Profiling indigenous lead-reducing bacteria from Tempe Lake, South Sulawesi, Indonesia as bioremediation agents. Biodiversitas 21: 4778-4786. The pollution of heavy metals by anthropogenic activities in freshwater, especially in lakes, reduces the quality and endangers the existence of aquatic flora and fauna. This happens in Lake Tempe, which is located, in South Sulawesi, Indonesia. The study aimed to evaluate the content of heavy metals, isolation, and identify a lead reduction of indigenous bacteria as candidates for bioremediation agents. It began by examining the level of cadmium (Cd), copper (Cu), and lead (Pb) in seven sampling sites of Tempe Lake. Furthermore, propagation and isolation, morphological observation, laboratory-scale reduction tests, and species identification of potential bacteria reduction Pb were performed by using 16s rDNA. The reduction test to Pb was measured using Atomic Absorption Spectrometry (AAS), and the research data were analyzed using One-Way ANOVA with the Duncan advanced method. The results showed that the Cd, Cu, and Pb content of the seven sampling points exceeded the water quality standard Following the Indonesian Government Regulation (IGR) and WHO standards. Pb has the highest concentration among the other heavy metals in the water sample reaching 0.40 mg/L. Four bacterial isolates could grow on Pb-enriched media (isolates A, B, C, and D). The Bacillus form was found in two different species bacteria (isolate A and D), while the cocci shape was found in two other species (isolate B and C). Those all Gram-negative bacteria were observed to be a reduction to Pb exposure within a bacterial medium. Interestingly, these indigenous bacteria could decrease Pb concentration ranging from 7.45 to 3.48 ppm for seven days. Furthermore, the sequencing data showed that the indigenous bacteria (isolate D) had a 99.90% similarity to the Comamonas testosteroni strain S-2 constructed using Neighbor-joining phylogenetic analysis. Therefore, the indigenous bacteria C. testosteroni strain S-2 from Tempe lake may be proposed as a bioremediation agent to reduce Pb the water contaminants.AHMAD YANIMOHAMAD AMINFatchur RohmanENDANG SUARSINIWIRA EKA PUTRAMBI & UNS Soloarticlebioremediation, comamonas testosteroni, indigenous bacteria, lake tempe, lead-reductionBiology (General)QH301-705.5ENBiodiversitas, Vol 21, Iss 10 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	bioremediation, comamonas testosteroni, indigenous bacteria, lake tempe, lead-reduction Biology (General) QH301-705.5
spellingShingle	bioremediation, comamonas testosteroni, indigenous bacteria, lake tempe, lead-reduction Biology (General) QH301-705.5 AHMAD YANI MOHAMAD AMIN Fatchur Rohman ENDANG SUARSINI WIRA EKA PUTRA Profiling indigenous lead-reducing bacteria from Tempe Lake, South Sulawesi, Indonesia as bioremediation agents
description	Abstract. Yani A, Amin M, Rohman F, Suarsini E, Putra WE. 2020. Profiling indigenous lead-reducing bacteria from Tempe Lake, South Sulawesi, Indonesia as bioremediation agents. Biodiversitas 21: 4778-4786. The pollution of heavy metals by anthropogenic activities in freshwater, especially in lakes, reduces the quality and endangers the existence of aquatic flora and fauna. This happens in Lake Tempe, which is located, in South Sulawesi, Indonesia. The study aimed to evaluate the content of heavy metals, isolation, and identify a lead reduction of indigenous bacteria as candidates for bioremediation agents. It began by examining the level of cadmium (Cd), copper (Cu), and lead (Pb) in seven sampling sites of Tempe Lake. Furthermore, propagation and isolation, morphological observation, laboratory-scale reduction tests, and species identification of potential bacteria reduction Pb were performed by using 16s rDNA. The reduction test to Pb was measured using Atomic Absorption Spectrometry (AAS), and the research data were analyzed using One-Way ANOVA with the Duncan advanced method. The results showed that the Cd, Cu, and Pb content of the seven sampling points exceeded the water quality standard Following the Indonesian Government Regulation (IGR) and WHO standards. Pb has the highest concentration among the other heavy metals in the water sample reaching 0.40 mg/L. Four bacterial isolates could grow on Pb-enriched media (isolates A, B, C, and D). The Bacillus form was found in two different species bacteria (isolate A and D), while the cocci shape was found in two other species (isolate B and C). Those all Gram-negative bacteria were observed to be a reduction to Pb exposure within a bacterial medium. Interestingly, these indigenous bacteria could decrease Pb concentration ranging from 7.45 to 3.48 ppm for seven days. Furthermore, the sequencing data showed that the indigenous bacteria (isolate D) had a 99.90% similarity to the Comamonas testosteroni strain S-2 constructed using Neighbor-joining phylogenetic analysis. Therefore, the indigenous bacteria C. testosteroni strain S-2 from Tempe lake may be proposed as a bioremediation agent to reduce Pb the water contaminants.
format	article
author	AHMAD YANI MOHAMAD AMIN Fatchur Rohman ENDANG SUARSINI WIRA EKA PUTRA
author_facet	AHMAD YANI MOHAMAD AMIN Fatchur Rohman ENDANG SUARSINI WIRA EKA PUTRA
author_sort	AHMAD YANI
title	Profiling indigenous lead-reducing bacteria from Tempe Lake, South Sulawesi, Indonesia as bioremediation agents
title_short	Profiling indigenous lead-reducing bacteria from Tempe Lake, South Sulawesi, Indonesia as bioremediation agents
title_full	Profiling indigenous lead-reducing bacteria from Tempe Lake, South Sulawesi, Indonesia as bioremediation agents
title_fullStr	Profiling indigenous lead-reducing bacteria from Tempe Lake, South Sulawesi, Indonesia as bioremediation agents
title_full_unstemmed	Profiling indigenous lead-reducing bacteria from Tempe Lake, South Sulawesi, Indonesia as bioremediation agents
title_sort	profiling indigenous lead-reducing bacteria from tempe lake, south sulawesi, indonesia as bioremediation agents
publisher	MBI & UNS Solo
publishDate	2020
url	https://doaj.org/article/385fb98f93864584839fb6696af0f019
work_keys_str_mv	AT ahmadyani profilingindigenousleadreducingbacteriafromtempelakesouthsulawesiindonesiaasbioremediationagents AT mohamadamin profilingindigenousleadreducingbacteriafromtempelakesouthsulawesiindonesiaasbioremediationagents AT fatchurrohman profilingindigenousleadreducingbacteriafromtempelakesouthsulawesiindonesiaasbioremediationagents AT endangsuarsini profilingindigenousleadreducingbacteriafromtempelakesouthsulawesiindonesiaasbioremediationagents AT wiraekaputra profilingindigenousleadreducingbacteriafromtempelakesouthsulawesiindonesiaasbioremediationagents
_version_	1718418451443220480

Profiling indigenous lead-reducing bacteria from Tempe Lake, South Sulawesi, Indonesia as bioremediation agents

Ejemplares similares