Uridine Ameliorates Dextran Sulfate Sodium (DSS)-Induced Colitis in Mice

Abstract Uridine, one of the four components that comprise RNA, has attracted attention as a novel therapeutic modulator of inflammation. However, very little is known about its effect on intestinal inflammation. The aim of the present study was to investigate the potential protective effect of intr...

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Autores principales:	Manish Kumar Jeengar, Dinesh Thummuri, Mattias Magnusson, V. G. M. Naidu, Srinivas Uppugunduri
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/3862d3286e584bec9bb0e353467355cf
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spelling	oai:doaj.org-article:3862d3286e584bec9bb0e353467355cf2021-12-02T16:07:01ZUridine Ameliorates Dextran Sulfate Sodium (DSS)-Induced Colitis in Mice10.1038/s41598-017-04041-92045-2322https://doaj.org/article/3862d3286e584bec9bb0e353467355cf2017-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-04041-9https://doaj.org/toc/2045-2322Abstract Uridine, one of the four components that comprise RNA, has attracted attention as a novel therapeutic modulator of inflammation. However, very little is known about its effect on intestinal inflammation. The aim of the present study was to investigate the potential protective effect of intracolonic administered uridine against DSS induced colitis in male C57BL/6 mice. Intracolonic instillation of 3 doses of uridine 1 mg/Kg (lower dose), 5 mg/Kg (medium dose), and 10 mg/Kg (higher dose) in saline was performed daily. Uridine at medium and high dose significantly reduced the severity of colitis (DAI score) and alleviated the macroscopic and microscopic signs of the disease. The levels of proinflammatory cytokines IL-6, IL-1β and TNF in serum as well as mRNA expression in colon were significantly reduced in the uridine treated groups. Moreover, colon tissue myloperoxidase activities, protein expression of IL-6, TNF- α, COX-2, P-NFkB and P-Ikk-βα in the colon tissues were significantly reduced in medium and high dose groups. These findings demonstrated that local administration of uridine alleviated experimental colitis in male C57BL/6 mice accompanied by the inhibition of neutrophil infiltration and NF-κB signaling. Thus, Uridine may be a promising candidate for future use in the treatment of inflammatory bowel disease.Manish Kumar JeengarDinesh ThummuriMattias MagnussonV. G. M. NaiduSrinivas UppugunduriNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-10 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Manish Kumar Jeengar Dinesh Thummuri Mattias Magnusson V. G. M. Naidu Srinivas Uppugunduri Uridine Ameliorates Dextran Sulfate Sodium (DSS)-Induced Colitis in Mice
description	Abstract Uridine, one of the four components that comprise RNA, has attracted attention as a novel therapeutic modulator of inflammation. However, very little is known about its effect on intestinal inflammation. The aim of the present study was to investigate the potential protective effect of intracolonic administered uridine against DSS induced colitis in male C57BL/6 mice. Intracolonic instillation of 3 doses of uridine 1 mg/Kg (lower dose), 5 mg/Kg (medium dose), and 10 mg/Kg (higher dose) in saline was performed daily. Uridine at medium and high dose significantly reduced the severity of colitis (DAI score) and alleviated the macroscopic and microscopic signs of the disease. The levels of proinflammatory cytokines IL-6, IL-1β and TNF in serum as well as mRNA expression in colon were significantly reduced in the uridine treated groups. Moreover, colon tissue myloperoxidase activities, protein expression of IL-6, TNF- α, COX-2, P-NFkB and P-Ikk-βα in the colon tissues were significantly reduced in medium and high dose groups. These findings demonstrated that local administration of uridine alleviated experimental colitis in male C57BL/6 mice accompanied by the inhibition of neutrophil infiltration and NF-κB signaling. Thus, Uridine may be a promising candidate for future use in the treatment of inflammatory bowel disease.
format	article
author	Manish Kumar Jeengar Dinesh Thummuri Mattias Magnusson V. G. M. Naidu Srinivas Uppugunduri
author_facet	Manish Kumar Jeengar Dinesh Thummuri Mattias Magnusson V. G. M. Naidu Srinivas Uppugunduri
author_sort	Manish Kumar Jeengar
title	Uridine Ameliorates Dextran Sulfate Sodium (DSS)-Induced Colitis in Mice
title_short	Uridine Ameliorates Dextran Sulfate Sodium (DSS)-Induced Colitis in Mice
title_full	Uridine Ameliorates Dextran Sulfate Sodium (DSS)-Induced Colitis in Mice
title_fullStr	Uridine Ameliorates Dextran Sulfate Sodium (DSS)-Induced Colitis in Mice
title_full_unstemmed	Uridine Ameliorates Dextran Sulfate Sodium (DSS)-Induced Colitis in Mice
title_sort	uridine ameliorates dextran sulfate sodium (dss)-induced colitis in mice
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/3862d3286e584bec9bb0e353467355cf
work_keys_str_mv	AT manishkumarjeengar uridineamelioratesdextransulfatesodiumdssinducedcolitisinmice AT dineshthummuri uridineamelioratesdextransulfatesodiumdssinducedcolitisinmice AT mattiasmagnusson uridineamelioratesdextransulfatesodiumdssinducedcolitisinmice AT vgmnaidu uridineamelioratesdextransulfatesodiumdssinducedcolitisinmice AT srinivasuppugunduri uridineamelioratesdextransulfatesodiumdssinducedcolitisinmice
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Uridine Ameliorates Dextran Sulfate Sodium (DSS)-Induced Colitis in Mice

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