Predominance of blaTEM and tetA genes in antibiotic-resistant Escherichia coli isolates from Laguna Lake, Philippines

Lakes are one of the sinks of antibiotic-resistant bacteria (ARB) and antibiotic resistance genes (ARGs); however, information on ARB and ARGs in lakes in the Philippines is scarce. In this study, Escherichia coli was isolated from the largest freshwater lake in the Philippines, Laguna Lake, to dete...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Daile Meek Salvador-Membreve, Windell L. Rivera
Formato:	article
Lenguaje:	EN
Publicado:	IWA Publishing 2021
Materias:	lake water quality multidrug resistance philippines surface water thermotolerant e. coli Environmental technology. Sanitary engineering TD1-1066
Acceso en línea:	https://doaj.org/article/3862f8c8456b445888e227ea553d17c0
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:3862f8c8456b445888e227ea553d17c0
record_format	dspace
spelling	oai:doaj.org-article:3862f8c8456b445888e227ea553d17c02021-11-05T19:40:32ZPredominance of blaTEM and tetA genes in antibiotic-resistant Escherichia coli isolates from Laguna Lake, Philippines2043-90832408-936210.2166/washdev.2021.067https://doaj.org/article/3862f8c8456b445888e227ea553d17c02021-09-01T00:00:00Zhttp://washdev.iwaponline.com/content/11/5/814https://doaj.org/toc/2043-9083https://doaj.org/toc/2408-9362Lakes are one of the sinks of antibiotic-resistant bacteria (ARB) and antibiotic resistance genes (ARGs); however, information on ARB and ARGs in lakes in the Philippines is scarce. In this study, Escherichia coli was isolated from the largest freshwater lake in the Philippines, Laguna Lake, to detect antibiotic resistance and the presence of ARGs. Broth microdilution assay (BMA) and molecular identification of five environmentally prevalent ARGs (strA, blaCTX-M, blaSHV, blaTEM, and tetA) were performed. The majority (75.70%) of the isolates harbored at least one of the targeted antibiotic genes. Multiplex PCR detected about 49.07% of the isolates had genes for extended-spectrum β-lactamases (ESBL), which were mostly represented by blaTEM (47.66%). The genes strA and tetA were observed in this study with detection frequencies of 29.91 and 45.33%, respectively. About 95.69% of thermotolerant E. coli isolates were non-susceptible to six different antibiotics using BMA. Nearly 37% of the isolates were found to be multidrug-resistant (MDR) with most isolates resistant to ampicillin (81.72%). Furthermore, the occurrence of ESBL genes was significantly correlated with tetA genes (P = 0.013). To date, this study is the first to report on the presence of MDR and thermotolerant E. coli in Laguna Lake, Philippines. HIGHLIGHTS First report of the ecological presence of thermotolerant and multidrug-resistant Escherichia coli (37%) in Laguna Lake.; Most (75.70%) isolates harbored at least one of the targeted antibiotic resistance genes (strA, blaTEM, blaCTX-M, blaSHV, and tetA).; First report of the occurrence of aminoglycoside resistant gene (strA) and tetracycline efflux gene (tetA) in waterborne E. coli isolated in the Philippines.;Daile Meek Salvador-MembreveWindell L. RiveraIWA Publishingarticlelake water qualitymultidrug resistancephilippinessurface waterthermotolerant e. coliEnvironmental technology. Sanitary engineeringTD1-1066ENJournal of Water, Sanitation and Hygiene for Development, Vol 11, Iss 5, Pp 814-823 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	lake water quality multidrug resistance philippines surface water thermotolerant e. coli Environmental technology. Sanitary engineering TD1-1066
spellingShingle	lake water quality multidrug resistance philippines surface water thermotolerant e. coli Environmental technology. Sanitary engineering TD1-1066 Daile Meek Salvador-Membreve Windell L. Rivera Predominance of blaTEM and tetA genes in antibiotic-resistant Escherichia coli isolates from Laguna Lake, Philippines
description	Lakes are one of the sinks of antibiotic-resistant bacteria (ARB) and antibiotic resistance genes (ARGs); however, information on ARB and ARGs in lakes in the Philippines is scarce. In this study, Escherichia coli was isolated from the largest freshwater lake in the Philippines, Laguna Lake, to detect antibiotic resistance and the presence of ARGs. Broth microdilution assay (BMA) and molecular identification of five environmentally prevalent ARGs (strA, blaCTX-M, blaSHV, blaTEM, and tetA) were performed. The majority (75.70%) of the isolates harbored at least one of the targeted antibiotic genes. Multiplex PCR detected about 49.07% of the isolates had genes for extended-spectrum β-lactamases (ESBL), which were mostly represented by blaTEM (47.66%). The genes strA and tetA were observed in this study with detection frequencies of 29.91 and 45.33%, respectively. About 95.69% of thermotolerant E. coli isolates were non-susceptible to six different antibiotics using BMA. Nearly 37% of the isolates were found to be multidrug-resistant (MDR) with most isolates resistant to ampicillin (81.72%). Furthermore, the occurrence of ESBL genes was significantly correlated with tetA genes (P = 0.013). To date, this study is the first to report on the presence of MDR and thermotolerant E. coli in Laguna Lake, Philippines. HIGHLIGHTS First report of the ecological presence of thermotolerant and multidrug-resistant Escherichia coli (37%) in Laguna Lake.; Most (75.70%) isolates harbored at least one of the targeted antibiotic resistance genes (strA, blaTEM, blaCTX-M, blaSHV, and tetA).; First report of the occurrence of aminoglycoside resistant gene (strA) and tetracycline efflux gene (tetA) in waterborne E. coli isolated in the Philippines.;
format	article
author	Daile Meek Salvador-Membreve Windell L. Rivera
author_facet	Daile Meek Salvador-Membreve Windell L. Rivera
author_sort	Daile Meek Salvador-Membreve
title	Predominance of blaTEM and tetA genes in antibiotic-resistant Escherichia coli isolates from Laguna Lake, Philippines
title_short	Predominance of blaTEM and tetA genes in antibiotic-resistant Escherichia coli isolates from Laguna Lake, Philippines
title_full	Predominance of blaTEM and tetA genes in antibiotic-resistant Escherichia coli isolates from Laguna Lake, Philippines
title_fullStr	Predominance of blaTEM and tetA genes in antibiotic-resistant Escherichia coli isolates from Laguna Lake, Philippines
title_full_unstemmed	Predominance of blaTEM and tetA genes in antibiotic-resistant Escherichia coli isolates from Laguna Lake, Philippines
title_sort	predominance of blatem and teta genes in antibiotic-resistant escherichia coli isolates from laguna lake, philippines
publisher	IWA Publishing
publishDate	2021
url	https://doaj.org/article/3862f8c8456b445888e227ea553d17c0
work_keys_str_mv	AT dailemeeksalvadormembreve predominanceofblatemandtetagenesinantibioticresistantescherichiacoliisolatesfromlagunalakephilippines AT windelllrivera predominanceofblatemandtetagenesinantibioticresistantescherichiacoliisolatesfromlagunalakephilippines
_version_	1718444031086690304

Predominance of blaTEM and tetA genes in antibiotic-resistant Escherichia coli isolates from Laguna Lake, Philippines

Ejemplares similares