Expression of inflammatory interleukins and selected miRNAs in non-small cell lung cancer

Abstract Tumours are characterised by an ability to avoid immune destruction and the presence of cancer-associated inflammation. Better understanding of the link between lung cancer and such inflammation is vital for early detection and personalized treatment. Thus, we examined the mRNA expression o...

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Autores principales:	Agata Dutkowska, Bartosz Szmyd, Marcin Kaszkowiak, Daria Domańska-Senderowska, Dorota Pastuszak-Lewandoska, Ewa Brzeziańska-Lasota, Jacek Kordiak, Adam Antczak
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/386aafec3c574a4bb6f72c73d0b0b62c
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spelling	oai:doaj.org-article:386aafec3c574a4bb6f72c73d0b0b62c2021-12-02T13:20:14ZExpression of inflammatory interleukins and selected miRNAs in non-small cell lung cancer10.1038/s41598-021-84408-12045-2322https://doaj.org/article/386aafec3c574a4bb6f72c73d0b0b62c2021-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-84408-1https://doaj.org/toc/2045-2322Abstract Tumours are characterised by an ability to avoid immune destruction and the presence of cancer-associated inflammation. Better understanding of the link between lung cancer and such inflammation is vital for early detection and personalized treatment. Thus, we examined the mRNA expression of interleukins IL-1β, IL-6, IL-17 and miR-9, miR-122 as potential useful biomarkers of NSCLC. Tumour tissues, non-cancerous tissue and blood samples were collected from 39 patients with primary NSCLC undergoing surgical treatment. The selected RNA was isolated from tissue samples and selected miRNAs from peripheral blood exosomes. This RNA was transcribed to cDNA and quantified using RT-qPCR. Significantly higher expression of the selected interleukins was observed in non-cancerous than tumour tissue, and IL-6 was significantly higher in the tumour tissue of patients with a history of ≤ 40 pack-years (PYs) (2.197, IQR: 0.821–4.415) than in those with > 40 PYs (0.461, IQR: 0.372–0.741; p = 0.037). It is clear that inflammatory processes play a role in NSCLC, as indicated by the upregulation of IL-1β and IL-6 in tumour and adjacent tissue, and that smoking has a strong influence on inflammation in tumourigenesis, demonstrated by the upregulation of IL-6 in tumour samples among patients with ≤ 40 PYs compared to > 40 PYs.Agata DutkowskaBartosz SzmydMarcin KaszkowiakDaria Domańska-SenderowskaDorota Pastuszak-LewandoskaEwa Brzeziańska-LasotaJacek KordiakAdam AntczakNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Agata Dutkowska Bartosz Szmyd Marcin Kaszkowiak Daria Domańska-Senderowska Dorota Pastuszak-Lewandoska Ewa Brzeziańska-Lasota Jacek Kordiak Adam Antczak Expression of inflammatory interleukins and selected miRNAs in non-small cell lung cancer
description	Abstract Tumours are characterised by an ability to avoid immune destruction and the presence of cancer-associated inflammation. Better understanding of the link between lung cancer and such inflammation is vital for early detection and personalized treatment. Thus, we examined the mRNA expression of interleukins IL-1β, IL-6, IL-17 and miR-9, miR-122 as potential useful biomarkers of NSCLC. Tumour tissues, non-cancerous tissue and blood samples were collected from 39 patients with primary NSCLC undergoing surgical treatment. The selected RNA was isolated from tissue samples and selected miRNAs from peripheral blood exosomes. This RNA was transcribed to cDNA and quantified using RT-qPCR. Significantly higher expression of the selected interleukins was observed in non-cancerous than tumour tissue, and IL-6 was significantly higher in the tumour tissue of patients with a history of ≤ 40 pack-years (PYs) (2.197, IQR: 0.821–4.415) than in those with > 40 PYs (0.461, IQR: 0.372–0.741; p = 0.037). It is clear that inflammatory processes play a role in NSCLC, as indicated by the upregulation of IL-1β and IL-6 in tumour and adjacent tissue, and that smoking has a strong influence on inflammation in tumourigenesis, demonstrated by the upregulation of IL-6 in tumour samples among patients with ≤ 40 PYs compared to > 40 PYs.
format	article
author	Agata Dutkowska Bartosz Szmyd Marcin Kaszkowiak Daria Domańska-Senderowska Dorota Pastuszak-Lewandoska Ewa Brzeziańska-Lasota Jacek Kordiak Adam Antczak
author_facet	Agata Dutkowska Bartosz Szmyd Marcin Kaszkowiak Daria Domańska-Senderowska Dorota Pastuszak-Lewandoska Ewa Brzeziańska-Lasota Jacek Kordiak Adam Antczak
author_sort	Agata Dutkowska
title	Expression of inflammatory interleukins and selected miRNAs in non-small cell lung cancer
title_short	Expression of inflammatory interleukins and selected miRNAs in non-small cell lung cancer
title_full	Expression of inflammatory interleukins and selected miRNAs in non-small cell lung cancer
title_fullStr	Expression of inflammatory interleukins and selected miRNAs in non-small cell lung cancer
title_full_unstemmed	Expression of inflammatory interleukins and selected miRNAs in non-small cell lung cancer
title_sort	expression of inflammatory interleukins and selected mirnas in non-small cell lung cancer
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/386aafec3c574a4bb6f72c73d0b0b62c
work_keys_str_mv	AT agatadutkowska expressionofinflammatoryinterleukinsandselectedmirnasinnonsmallcelllungcancer AT bartoszszmyd expressionofinflammatoryinterleukinsandselectedmirnasinnonsmallcelllungcancer AT marcinkaszkowiak expressionofinflammatoryinterleukinsandselectedmirnasinnonsmallcelllungcancer AT dariadomanskasenderowska expressionofinflammatoryinterleukinsandselectedmirnasinnonsmallcelllungcancer AT dorotapastuszaklewandoska expressionofinflammatoryinterleukinsandselectedmirnasinnonsmallcelllungcancer AT ewabrzezianskalasota expressionofinflammatoryinterleukinsandselectedmirnasinnonsmallcelllungcancer AT jacekkordiak expressionofinflammatoryinterleukinsandselectedmirnasinnonsmallcelllungcancer AT adamantczak expressionofinflammatoryinterleukinsandselectedmirnasinnonsmallcelllungcancer
_version_	1718393203134038016

Expression of inflammatory interleukins and selected miRNAs in non-small cell lung cancer

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