Crucial ignored parameters on nanotoxicology: the importance of toxicity assay modifications and "cell vision".

Until now, the results of nanotoxicology research have shown that the interactions between nanoparticles (NPs) and cells are remarkably complex. In order to get a deep understanding of the NP-cell interactions, scientists have focused on the physicochemical effects. However, there are still consider...

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Autores principales:	Sophie Laurent, Carmen Burtea, Coralie Thirifays, Urs O Häfeli, Morteza Mahmoudi
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/386d863c97194657a96c9f7a044a4bbf
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spelling	oai:doaj.org-article:386d863c97194657a96c9f7a044a4bbf2021-11-18T07:30:37ZCrucial ignored parameters on nanotoxicology: the importance of toxicity assay modifications and "cell vision".1932-620310.1371/journal.pone.0029997https://doaj.org/article/386d863c97194657a96c9f7a044a4bbf2012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22253854/?tool=EBIhttps://doaj.org/toc/1932-6203Until now, the results of nanotoxicology research have shown that the interactions between nanoparticles (NPs) and cells are remarkably complex. In order to get a deep understanding of the NP-cell interactions, scientists have focused on the physicochemical effects. However, there are still considerable debates about the regulation of nanomaterials and the reported results are usually in contradictions. Here, we are going to introduce the potential key reasons for these conflicts. In this case, modification of conventional in vitro toxicity assays, is one of the crucial ignored matter in nanotoxicological sciences. More specifically, the conventional methods neglect important factors such as the sedimentation of NPs and absorption of proteins and other essential biomolecules onto the surface of NPs. Another ignored matter in nanotoxicological sciences is the effect of cell "vision" (i.e., cell type). In order to show the effects of these ignored subjects, we probed the effect of superparamagnetic iron oxide NPs (SPIONs), with various surface chemistries, on various cell lines. We found thatthe modification of conventional toxicity assays and the consideration of the "cell vision" concept are crucial matters to obtain reliable, and reproducible nanotoxicology data. These new concepts offer a suitable way to obtain a deep understanding on the cell-NP interactions. In addition, by consideration of these ignored factors, the conflict of future toxicological reports would be significantly decreased.Sophie LaurentCarmen BurteaCoralie ThirifaysUrs O HäfeliMorteza MahmoudiPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 1, p e29997 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Sophie Laurent Carmen Burtea Coralie Thirifays Urs O Häfeli Morteza Mahmoudi Crucial ignored parameters on nanotoxicology: the importance of toxicity assay modifications and "cell vision".
description	Until now, the results of nanotoxicology research have shown that the interactions between nanoparticles (NPs) and cells are remarkably complex. In order to get a deep understanding of the NP-cell interactions, scientists have focused on the physicochemical effects. However, there are still considerable debates about the regulation of nanomaterials and the reported results are usually in contradictions. Here, we are going to introduce the potential key reasons for these conflicts. In this case, modification of conventional in vitro toxicity assays, is one of the crucial ignored matter in nanotoxicological sciences. More specifically, the conventional methods neglect important factors such as the sedimentation of NPs and absorption of proteins and other essential biomolecules onto the surface of NPs. Another ignored matter in nanotoxicological sciences is the effect of cell "vision" (i.e., cell type). In order to show the effects of these ignored subjects, we probed the effect of superparamagnetic iron oxide NPs (SPIONs), with various surface chemistries, on various cell lines. We found thatthe modification of conventional toxicity assays and the consideration of the "cell vision" concept are crucial matters to obtain reliable, and reproducible nanotoxicology data. These new concepts offer a suitable way to obtain a deep understanding on the cell-NP interactions. In addition, by consideration of these ignored factors, the conflict of future toxicological reports would be significantly decreased.
format	article
author	Sophie Laurent Carmen Burtea Coralie Thirifays Urs O Häfeli Morteza Mahmoudi
author_facet	Sophie Laurent Carmen Burtea Coralie Thirifays Urs O Häfeli Morteza Mahmoudi
author_sort	Sophie Laurent
title	Crucial ignored parameters on nanotoxicology: the importance of toxicity assay modifications and "cell vision".
title_short	Crucial ignored parameters on nanotoxicology: the importance of toxicity assay modifications and "cell vision".
title_full	Crucial ignored parameters on nanotoxicology: the importance of toxicity assay modifications and "cell vision".
title_fullStr	Crucial ignored parameters on nanotoxicology: the importance of toxicity assay modifications and "cell vision".
title_full_unstemmed	Crucial ignored parameters on nanotoxicology: the importance of toxicity assay modifications and "cell vision".
title_sort	crucial ignored parameters on nanotoxicology: the importance of toxicity assay modifications and "cell vision".
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/386d863c97194657a96c9f7a044a4bbf
work_keys_str_mv	AT sophielaurent crucialignoredparametersonnanotoxicologytheimportanceoftoxicityassaymodificationsandcellvision AT carmenburtea crucialignoredparametersonnanotoxicologytheimportanceoftoxicityassaymodificationsandcellvision AT coraliethirifays crucialignoredparametersonnanotoxicologytheimportanceoftoxicityassaymodificationsandcellvision AT ursohafeli crucialignoredparametersonnanotoxicologytheimportanceoftoxicityassaymodificationsandcellvision AT mortezamahmoudi crucialignoredparametersonnanotoxicologytheimportanceoftoxicityassaymodificationsandcellvision
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Crucial ignored parameters on nanotoxicology: the importance of toxicity assay modifications and "cell vision".

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