A novel approach to create an antibacterial surface using titanium dioxide and a combination of dip-pen nanolithography and soft lithography

Abstract Soft lithography and Dip-Pen Nanolithography (DPN) are techniques that have been used to modify the surface of biomaterials. Modified surfaces play a role in reducing bacterial adhesion and biofilm formation. Also, titanium dioxide has been reported as an antibacterial substance due to its...

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Autores principales:	Santiago Arango-Santander, Alejandro Pelaez-Vargas, Sidónio C. Freitas, Claudia García
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Soft Lithography 316L Stainless Steel Plate (SS316L) Micropatterned Surfaces TiO2 Concentration Bacterial Adhesion Medicine R Science Q
Acceso en línea:	https://doaj.org/article/386f76427c6842939d47fd4c11260b53
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spelling	oai:doaj.org-article:386f76427c6842939d47fd4c11260b532021-12-02T11:40:16ZA novel approach to create an antibacterial surface using titanium dioxide and a combination of dip-pen nanolithography and soft lithography10.1038/s41598-018-34198-w2045-2322https://doaj.org/article/386f76427c6842939d47fd4c11260b532018-10-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-34198-whttps://doaj.org/toc/2045-2322Abstract Soft lithography and Dip-Pen Nanolithography (DPN) are techniques that have been used to modify the surface of biomaterials. Modified surfaces play a role in reducing bacterial adhesion and biofilm formation. Also, titanium dioxide has been reported as an antibacterial substance due to its photocatalytic effect. This work aimed at creating patterns on model surfaces using DPN and soft lithography combined with titanium dioxide to create functional antibacterial micropatterned surfaces, which were tested against Streptococcus mutans. DPN was used to create a master pattern onto a model surface and microstamping was performed to duplicate and transfer such patterns to medical-grade stainless steel 316L using a suspension of TiO2. Modified SS316L plates were subjected to UVA black light as photocatalytic activator. Patterns were characterized by atomic force microscopy and biologically evaluated using S. mutans. A significant reduction of up to 60% in bacterial adhesion to TiO2 -coated and -micropatterned surfaces was observed. Moreover, both TiO2 surfaces reduced the viability of adhered bacteria after UV exposure. TiO2 micropatterned demonstrated a synergic effect between physical and chemical modification against S. mutans. This dual effect was enhanced by increasing TiO2 concentration. This novel approach may be a promising alternative to reduce bacterial adhesion to surfaces.Santiago Arango-SantanderAlejandro Pelaez-VargasSidónio C. FreitasClaudia GarcíaNature PortfolioarticleSoft Lithography316L Stainless Steel Plate (SS316L)Micropatterned SurfacesTiO2 ConcentrationBacterial AdhesionMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-10 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Soft Lithography 316L Stainless Steel Plate (SS316L) Micropatterned Surfaces TiO2 Concentration Bacterial Adhesion Medicine R Science Q
spellingShingle	Soft Lithography 316L Stainless Steel Plate (SS316L) Micropatterned Surfaces TiO2 Concentration Bacterial Adhesion Medicine R Science Q Santiago Arango-Santander Alejandro Pelaez-Vargas Sidónio C. Freitas Claudia García A novel approach to create an antibacterial surface using titanium dioxide and a combination of dip-pen nanolithography and soft lithography
description	Abstract Soft lithography and Dip-Pen Nanolithography (DPN) are techniques that have been used to modify the surface of biomaterials. Modified surfaces play a role in reducing bacterial adhesion and biofilm formation. Also, titanium dioxide has been reported as an antibacterial substance due to its photocatalytic effect. This work aimed at creating patterns on model surfaces using DPN and soft lithography combined with titanium dioxide to create functional antibacterial micropatterned surfaces, which were tested against Streptococcus mutans. DPN was used to create a master pattern onto a model surface and microstamping was performed to duplicate and transfer such patterns to medical-grade stainless steel 316L using a suspension of TiO2. Modified SS316L plates were subjected to UVA black light as photocatalytic activator. Patterns were characterized by atomic force microscopy and biologically evaluated using S. mutans. A significant reduction of up to 60% in bacterial adhesion to TiO2 -coated and -micropatterned surfaces was observed. Moreover, both TiO2 surfaces reduced the viability of adhered bacteria after UV exposure. TiO2 micropatterned demonstrated a synergic effect between physical and chemical modification against S. mutans. This dual effect was enhanced by increasing TiO2 concentration. This novel approach may be a promising alternative to reduce bacterial adhesion to surfaces.
format	article
author	Santiago Arango-Santander Alejandro Pelaez-Vargas Sidónio C. Freitas Claudia García
author_facet	Santiago Arango-Santander Alejandro Pelaez-Vargas Sidónio C. Freitas Claudia García
author_sort	Santiago Arango-Santander
title	A novel approach to create an antibacterial surface using titanium dioxide and a combination of dip-pen nanolithography and soft lithography
title_short	A novel approach to create an antibacterial surface using titanium dioxide and a combination of dip-pen nanolithography and soft lithography
title_full	A novel approach to create an antibacterial surface using titanium dioxide and a combination of dip-pen nanolithography and soft lithography
title_fullStr	A novel approach to create an antibacterial surface using titanium dioxide and a combination of dip-pen nanolithography and soft lithography
title_full_unstemmed	A novel approach to create an antibacterial surface using titanium dioxide and a combination of dip-pen nanolithography and soft lithography
title_sort	novel approach to create an antibacterial surface using titanium dioxide and a combination of dip-pen nanolithography and soft lithography
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/386f76427c6842939d47fd4c11260b53
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A novel approach to create an antibacterial surface using titanium dioxide and a combination of dip-pen nanolithography and soft lithography

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