Effects of high-frequency nanosecond pulses on prostate cancer cells

Abstract Electroporation with pulsed electric fields show a potential to be applied as an experimental focal therapy of tumors. Sub-microsecond regime of electric pulses displays unique electrophysical features operative in cells and membranes. Recently, MHz compression of nanosecond pulses electric...

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Autores principales:	Aleksander Kiełbik, Wojciech Szlasa, Vitalij Novickij, Anna Szewczyk, Magdalena Maciejewska, Jolanta Saczko, Julita Kulbacka
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/3872a8066eab4b84beacd7b0fd67424a
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spelling	oai:doaj.org-article:3872a8066eab4b84beacd7b0fd67424a2021-12-02T14:53:34ZEffects of high-frequency nanosecond pulses on prostate cancer cells10.1038/s41598-021-95180-72045-2322https://doaj.org/article/3872a8066eab4b84beacd7b0fd67424a2021-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-95180-7https://doaj.org/toc/2045-2322Abstract Electroporation with pulsed electric fields show a potential to be applied as an experimental focal therapy of tumors. Sub-microsecond regime of electric pulses displays unique electrophysical features operative in cells and membranes. Recently, MHz compression of nanosecond pulses electric fields (nsPEFs) bursts proved to enhance the effectiveness of the therapy. High morbidity of prostate cancer (PCa) and risk of overtreatment associated with this malignancy call for new minimal-invasive treatment alternative. Herein we present the in vitro study for developing applications based on this new technology. In this study, we used flow cytometric analysis, cell viability assay, caspase activity analysis, wound healing assay, confocal microscopy study, and immunofluorescence to investigate the biological effect of high-frequency nsPEFs on PCa cells. Our results show that high-frequency nsPEFs induces the permeabilization and cell death of PCa cells. The cytotoxicity is significantly enhanced in MHz compression of pulses and with the presence of extracellular Ca2+. High-frequency nsPEFs trigger changes in PCa cells’ cytoskeleton and their mobility. The presented data show a therapeutic potential of high-frequency nsPEFs in a PCa setting. The sub-microsecond regime of pulses can potentially be applied in nanosecond electroporation protocols for PCa treatment.Aleksander KiełbikWojciech SzlasaVitalij NovickijAnna SzewczykMagdalena MaciejewskaJolanta SaczkoJulita KulbackaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Aleksander Kiełbik Wojciech Szlasa Vitalij Novickij Anna Szewczyk Magdalena Maciejewska Jolanta Saczko Julita Kulbacka Effects of high-frequency nanosecond pulses on prostate cancer cells
description	Abstract Electroporation with pulsed electric fields show a potential to be applied as an experimental focal therapy of tumors. Sub-microsecond regime of electric pulses displays unique electrophysical features operative in cells and membranes. Recently, MHz compression of nanosecond pulses electric fields (nsPEFs) bursts proved to enhance the effectiveness of the therapy. High morbidity of prostate cancer (PCa) and risk of overtreatment associated with this malignancy call for new minimal-invasive treatment alternative. Herein we present the in vitro study for developing applications based on this new technology. In this study, we used flow cytometric analysis, cell viability assay, caspase activity analysis, wound healing assay, confocal microscopy study, and immunofluorescence to investigate the biological effect of high-frequency nsPEFs on PCa cells. Our results show that high-frequency nsPEFs induces the permeabilization and cell death of PCa cells. The cytotoxicity is significantly enhanced in MHz compression of pulses and with the presence of extracellular Ca2+. High-frequency nsPEFs trigger changes in PCa cells’ cytoskeleton and their mobility. The presented data show a therapeutic potential of high-frequency nsPEFs in a PCa setting. The sub-microsecond regime of pulses can potentially be applied in nanosecond electroporation protocols for PCa treatment.
format	article
author	Aleksander Kiełbik Wojciech Szlasa Vitalij Novickij Anna Szewczyk Magdalena Maciejewska Jolanta Saczko Julita Kulbacka
author_facet	Aleksander Kiełbik Wojciech Szlasa Vitalij Novickij Anna Szewczyk Magdalena Maciejewska Jolanta Saczko Julita Kulbacka
author_sort	Aleksander Kiełbik
title	Effects of high-frequency nanosecond pulses on prostate cancer cells
title_short	Effects of high-frequency nanosecond pulses on prostate cancer cells
title_full	Effects of high-frequency nanosecond pulses on prostate cancer cells
title_fullStr	Effects of high-frequency nanosecond pulses on prostate cancer cells
title_full_unstemmed	Effects of high-frequency nanosecond pulses on prostate cancer cells
title_sort	effects of high-frequency nanosecond pulses on prostate cancer cells
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/3872a8066eab4b84beacd7b0fd67424a
work_keys_str_mv	AT aleksanderkiełbik effectsofhighfrequencynanosecondpulsesonprostatecancercells AT wojciechszlasa effectsofhighfrequencynanosecondpulsesonprostatecancercells AT vitalijnovickij effectsofhighfrequencynanosecondpulsesonprostatecancercells AT annaszewczyk effectsofhighfrequencynanosecondpulsesonprostatecancercells AT magdalenamaciejewska effectsofhighfrequencynanosecondpulsesonprostatecancercells AT jolantasaczko effectsofhighfrequencynanosecondpulsesonprostatecancercells AT julitakulbacka effectsofhighfrequencynanosecondpulsesonprostatecancercells
_version_	1718389440268730368

Effects of high-frequency nanosecond pulses on prostate cancer cells

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