IgGs-Abzymes from the Sera of Patients with Systemic Lupus Erythematosus Hydrolyzed miRNAs

Evgeny A Ermakov,1,2 Evelina M Kabirova,1,2 Alexey E Sizikov,3 Valentina N Buneva,1,2 Georgy A Nevinsky1,2 1Institute of Chemical Biology and Fundamental Medicine, Siberian Division of Russian Academy of Sciences, Novosibirsk 630090, Russia; 2Novosibirsk State University, Novosibirsk 630090, Russia;...

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Autores principales:	Ermakov EA, Kabirova EM, Sizikov AE, Buneva VN, Nevinsky GA
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2020
Materias:	systemic lupus erythematosus igg-abzymes mirna hydrolysis autoimmune and inflammatory reactions Pathology RB1-214 Therapeutics. Pharmacology RM1-950
Acceso en línea:	https://doaj.org/article/38845fe254ec4535a7d3c8371d11050a
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spelling	oai:doaj.org-article:38845fe254ec4535a7d3c8371d11050a2021-12-02T12:25:26ZIgGs-Abzymes from the Sera of Patients with Systemic Lupus Erythematosus Hydrolyzed miRNAs1178-7031https://doaj.org/article/38845fe254ec4535a7d3c8371d11050a2020-10-01T00:00:00Zhttps://www.dovepress.com/iggs-abzymes-from-the-sera-of-patients-with-systemic-lupus-erythematos-peer-reviewed-article-JIRhttps://doaj.org/toc/1178-7031Evgeny A Ermakov,1,2 Evelina M Kabirova,1,2 Alexey E Sizikov,3 Valentina N Buneva,1,2 Georgy A Nevinsky1,2 1Institute of Chemical Biology and Fundamental Medicine, Siberian Division of Russian Academy of Sciences, Novosibirsk 630090, Russia; 2Novosibirsk State University, Novosibirsk 630090, Russia; 3Institute of Clinical Immunology, Siberian Branch of the Russian Academy of Sciences, Novosibirsk, RussiaCorrespondence: Georgy A Nevinsky Email nevinsky@niboch.nsc.ruBackground and Objectives: Systemic lupus erythematosus (SLE) is an inflammatory disease. The sera of SLE patients contain antibodies-abzymes hydrolyzing myelin basic protein (MBP), DNA, nucleotides, and oligosaccharides. The blood of SLE patients contains an increased amount of some specific miRNAs. This study aimed to analyze a possible hydrolysis of eight microRNAs found in the blood of SLE patients with high frequency by blood antibodies-abzymes.Patients and Methods: Using affinity chromatography of the serum proteins of SLE patients and healthy donors on protein G-Sepharose and following FPLC gel filtration, electrophoretically homogeneous IgG preparations containing no impurities of canonical RNases were obtained. These preparations were used to analyze their activity in the hydrolysis of eight miRNAs.Results: It was shown that SLE IgGs hydrolyze very efficiently four neuroregulatory miRNAs (miR-219-2-3p, miR-137, miR-219a-5p, and miR-9-5p) and four immunoregulatory miRNAs (miR-326, miR-21-3p, miR-155-5p, and miR-146a-3p). To demonstrate that the miRNAs hydrolysis is an intrinsic property of SLE IgGs, several rigid criteria were checked. Only some IgGs of healthy donors showed very weak, but reliably detectable activity in the hydrolysis miRNAs. The average activity of SLE patients IgGs according to median values is statistically significant 84.8-fold higher than that of healthy donors. The maximum and comparable average activity (RA) was observed in the hydrolysis of three miRAs: miR-9-5p, miR-155-5p, and miR-326. MiR-9-5p plays an important role in the development of lupus nephritis, while miR-326 activates the production of antibodies by B cells. The major and moderate specific sites of the hydrolysis of each miRNA were revealed. The hydrolysis of eight microRNAs was mostly site specific. Several SLE IgGs hydrolyzed some miRNAs demonstrating a combination of site-specific and non-specific splitting.Conclusion: Since inflammatory processes in SLE are associated with the change in miRNAs expression, the decrease in their concentration due to hydrolysis by autoantibodies-abzymes may be important for SLE pathogenesis.Keywords: systemic lupus erythematosus, IgG-abzymes, miRNA hydrolysis, autoimmune and inflammatory reactionsErmakov EAKabirova EMSizikov AEBuneva VNNevinsky GADove Medical Pressarticlesystemic lupus erythematosusigg-abzymesmirna hydrolysisautoimmune and inflammatory reactionsPathologyRB1-214Therapeutics. PharmacologyRM1-950ENJournal of Inflammation Research, Vol Volume 13, Pp 681-699 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	systemic lupus erythematosus igg-abzymes mirna hydrolysis autoimmune and inflammatory reactions Pathology RB1-214 Therapeutics. Pharmacology RM1-950
spellingShingle	systemic lupus erythematosus igg-abzymes mirna hydrolysis autoimmune and inflammatory reactions Pathology RB1-214 Therapeutics. Pharmacology RM1-950 Ermakov EA Kabirova EM Sizikov AE Buneva VN Nevinsky GA IgGs-Abzymes from the Sera of Patients with Systemic Lupus Erythematosus Hydrolyzed miRNAs
description	Evgeny A Ermakov,1,2 Evelina M Kabirova,1,2 Alexey E Sizikov,3 Valentina N Buneva,1,2 Georgy A Nevinsky1,2 1Institute of Chemical Biology and Fundamental Medicine, Siberian Division of Russian Academy of Sciences, Novosibirsk 630090, Russia; 2Novosibirsk State University, Novosibirsk 630090, Russia; 3Institute of Clinical Immunology, Siberian Branch of the Russian Academy of Sciences, Novosibirsk, RussiaCorrespondence: Georgy A Nevinsky Email nevinsky@niboch.nsc.ruBackground and Objectives: Systemic lupus erythematosus (SLE) is an inflammatory disease. The sera of SLE patients contain antibodies-abzymes hydrolyzing myelin basic protein (MBP), DNA, nucleotides, and oligosaccharides. The blood of SLE patients contains an increased amount of some specific miRNAs. This study aimed to analyze a possible hydrolysis of eight microRNAs found in the blood of SLE patients with high frequency by blood antibodies-abzymes.Patients and Methods: Using affinity chromatography of the serum proteins of SLE patients and healthy donors on protein G-Sepharose and following FPLC gel filtration, electrophoretically homogeneous IgG preparations containing no impurities of canonical RNases were obtained. These preparations were used to analyze their activity in the hydrolysis of eight miRNAs.Results: It was shown that SLE IgGs hydrolyze very efficiently four neuroregulatory miRNAs (miR-219-2-3p, miR-137, miR-219a-5p, and miR-9-5p) and four immunoregulatory miRNAs (miR-326, miR-21-3p, miR-155-5p, and miR-146a-3p). To demonstrate that the miRNAs hydrolysis is an intrinsic property of SLE IgGs, several rigid criteria were checked. Only some IgGs of healthy donors showed very weak, but reliably detectable activity in the hydrolysis miRNAs. The average activity of SLE patients IgGs according to median values is statistically significant 84.8-fold higher than that of healthy donors. The maximum and comparable average activity (RA) was observed in the hydrolysis of three miRAs: miR-9-5p, miR-155-5p, and miR-326. MiR-9-5p plays an important role in the development of lupus nephritis, while miR-326 activates the production of antibodies by B cells. The major and moderate specific sites of the hydrolysis of each miRNA were revealed. The hydrolysis of eight microRNAs was mostly site specific. Several SLE IgGs hydrolyzed some miRNAs demonstrating a combination of site-specific and non-specific splitting.Conclusion: Since inflammatory processes in SLE are associated with the change in miRNAs expression, the decrease in their concentration due to hydrolysis by autoantibodies-abzymes may be important for SLE pathogenesis.Keywords: systemic lupus erythematosus, IgG-abzymes, miRNA hydrolysis, autoimmune and inflammatory reactions
format	article
author	Ermakov EA Kabirova EM Sizikov AE Buneva VN Nevinsky GA
author_facet	Ermakov EA Kabirova EM Sizikov AE Buneva VN Nevinsky GA
author_sort	Ermakov EA
title	IgGs-Abzymes from the Sera of Patients with Systemic Lupus Erythematosus Hydrolyzed miRNAs
title_short	IgGs-Abzymes from the Sera of Patients with Systemic Lupus Erythematosus Hydrolyzed miRNAs
title_full	IgGs-Abzymes from the Sera of Patients with Systemic Lupus Erythematosus Hydrolyzed miRNAs
title_fullStr	IgGs-Abzymes from the Sera of Patients with Systemic Lupus Erythematosus Hydrolyzed miRNAs
title_full_unstemmed	IgGs-Abzymes from the Sera of Patients with Systemic Lupus Erythematosus Hydrolyzed miRNAs
title_sort	iggs-abzymes from the sera of patients with systemic lupus erythematosus hydrolyzed mirnas
publisher	Dove Medical Press
publishDate	2020
url	https://doaj.org/article/38845fe254ec4535a7d3c8371d11050a
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IgGs-Abzymes from the Sera of Patients with Systemic Lupus Erythematosus Hydrolyzed miRNAs

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