The neuroprotective effects of intramuscular insulin-like growth factor-I treatment in brain ischemic rats.

Brain ischemia leads to muscle inactivity-induced atrophy and may exacerbate motor function deficits. Intramuscular insulin-like growth factor I (IGF-I) injection has been shown to alleviate the brain ischemia-induced muscle atrophy and thus improve the motor function. Motor function is normally gau...

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Autores principales:	Heng-Chih Chang, Yea-Ru Yang, Paulus S Wang, Chia-Hua Kuo, Ray-Yau Wang
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/388666c2e65240e4a425d4f0cb493a4c
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spelling	oai:doaj.org-article:388666c2e65240e4a425d4f0cb493a4c2021-11-18T07:44:38ZThe neuroprotective effects of intramuscular insulin-like growth factor-I treatment in brain ischemic rats.1932-620310.1371/journal.pone.0064015https://doaj.org/article/388666c2e65240e4a425d4f0cb493a4c2013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23717526/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Brain ischemia leads to muscle inactivity-induced atrophy and may exacerbate motor function deficits. Intramuscular insulin-like growth factor I (IGF-I) injection has been shown to alleviate the brain ischemia-induced muscle atrophy and thus improve the motor function. Motor function is normally gauged by the integrity and coordination of the central nervous system and peripheral muscles. Whether brain ischemic regions are adaptively changed by the intramuscular IGF-I injection is not well understood. In this study, the effect of intramuscular IGF-I injection was examined on the central nervous system of brain ischemic rats. Rats were divided into 4 groups: sham control, brain ischemia control, brain ischemia with IGF-I treatment, and brain ischemia with IGF-I plus IGF-I receptor inhibitor treatment. Brain ischemia was induced by right middle cerebral artery occlusion. IGF-I and an IGF-1 receptor inhibitor were injected into the affected calf and anterior tibialis muscles of the treated rats for 4 times. There was an interval of 2 days between each injection. Motor function was examined and measured at the 24 hours and 7 days following a brain ischemia. The affected hind-limb muscles, sciatic nerve, lumbar spinal cord, and motor cortex were collected for examination after euthanizing the rats. IGF-I expression in the central nervous system and affected muscles were significantly decreased after brain ischemia. Intramuscular IGF-I injection increased the IGF-I expression in the affected muscles, sciatic nerve, lumbar spinal cord, and motor cortex. It also increased the p-Akt expression in the affected motor cortex. Furthermore, intramuscular IGF-I injection decreased the neuronal apoptosis and improved the motor function. However, co-administration of the IGF-I receptor inhibitor eliminated these effects. Intramuscular IGF-I injection after brain ischemia attenuated or reversed the decrease of IGF-I in both central and peripheral tissues, and these effects could contribute to neuroprotection and improve motor function.Heng-Chih ChangYea-Ru YangPaulus S WangChia-Hua KuoRay-Yau WangPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 5, p e64015 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Heng-Chih Chang Yea-Ru Yang Paulus S Wang Chia-Hua Kuo Ray-Yau Wang The neuroprotective effects of intramuscular insulin-like growth factor-I treatment in brain ischemic rats.
description	Brain ischemia leads to muscle inactivity-induced atrophy and may exacerbate motor function deficits. Intramuscular insulin-like growth factor I (IGF-I) injection has been shown to alleviate the brain ischemia-induced muscle atrophy and thus improve the motor function. Motor function is normally gauged by the integrity and coordination of the central nervous system and peripheral muscles. Whether brain ischemic regions are adaptively changed by the intramuscular IGF-I injection is not well understood. In this study, the effect of intramuscular IGF-I injection was examined on the central nervous system of brain ischemic rats. Rats were divided into 4 groups: sham control, brain ischemia control, brain ischemia with IGF-I treatment, and brain ischemia with IGF-I plus IGF-I receptor inhibitor treatment. Brain ischemia was induced by right middle cerebral artery occlusion. IGF-I and an IGF-1 receptor inhibitor were injected into the affected calf and anterior tibialis muscles of the treated rats for 4 times. There was an interval of 2 days between each injection. Motor function was examined and measured at the 24 hours and 7 days following a brain ischemia. The affected hind-limb muscles, sciatic nerve, lumbar spinal cord, and motor cortex were collected for examination after euthanizing the rats. IGF-I expression in the central nervous system and affected muscles were significantly decreased after brain ischemia. Intramuscular IGF-I injection increased the IGF-I expression in the affected muscles, sciatic nerve, lumbar spinal cord, and motor cortex. It also increased the p-Akt expression in the affected motor cortex. Furthermore, intramuscular IGF-I injection decreased the neuronal apoptosis and improved the motor function. However, co-administration of the IGF-I receptor inhibitor eliminated these effects. Intramuscular IGF-I injection after brain ischemia attenuated or reversed the decrease of IGF-I in both central and peripheral tissues, and these effects could contribute to neuroprotection and improve motor function.
format	article
author	Heng-Chih Chang Yea-Ru Yang Paulus S Wang Chia-Hua Kuo Ray-Yau Wang
author_facet	Heng-Chih Chang Yea-Ru Yang Paulus S Wang Chia-Hua Kuo Ray-Yau Wang
author_sort	Heng-Chih Chang
title	The neuroprotective effects of intramuscular insulin-like growth factor-I treatment in brain ischemic rats.
title_short	The neuroprotective effects of intramuscular insulin-like growth factor-I treatment in brain ischemic rats.
title_full	The neuroprotective effects of intramuscular insulin-like growth factor-I treatment in brain ischemic rats.
title_fullStr	The neuroprotective effects of intramuscular insulin-like growth factor-I treatment in brain ischemic rats.
title_full_unstemmed	The neuroprotective effects of intramuscular insulin-like growth factor-I treatment in brain ischemic rats.
title_sort	neuroprotective effects of intramuscular insulin-like growth factor-i treatment in brain ischemic rats.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/388666c2e65240e4a425d4f0cb493a4c
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The neuroprotective effects of intramuscular insulin-like growth factor-I treatment in brain ischemic rats.

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