Molecular Characteristics of Rifampin-Sensitive and -Resistant Isolates and Characteristics of rpoB Gene Mutations in Methicillin-Resistant Staphylococcus aureus

Yinjuan Guo,1 Bingjie Wang,1 Lulin Rao,1 Xinyi Wang,1 Huilin Zhao,1 Meilan Li,2 Fangyou Yu1 1Department of Clinical Laboratory, Shanghai Pulmonary Hospital, School of Medicine, Tongji University, Shanghai, 200433, People’s Republic of China; 2Respiratory Intensive Care Unit, Shanghai Pulmonary Hospi...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Guo Y, Wang B, Rao L, Wang X, Zhao H, Li M, Yu F
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2021
Materias:	mrsa rifampin rpob mutations mlst sccmec spa. Infectious and parasitic diseases RC109-216
Acceso en línea:	https://doaj.org/article/388afe49e0a14095b67b6c44911f5993
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:388afe49e0a14095b67b6c44911f5993
record_format	dspace
spelling	oai:doaj.org-article:388afe49e0a14095b67b6c44911f59932021-11-04T19:00:26ZMolecular Characteristics of Rifampin-Sensitive and -Resistant Isolates and Characteristics of rpoB Gene Mutations in Methicillin-Resistant Staphylococcus aureus1178-6973https://doaj.org/article/388afe49e0a14095b67b6c44911f59932021-11-01T00:00:00Zhttps://www.dovepress.com/molecular-characteristics-of-rifampin-sensitive-and--resistant-isolate-peer-reviewed-fulltext-article-IDRhttps://doaj.org/toc/1178-6973Yinjuan Guo,1 Bingjie Wang,1 Lulin Rao,1 Xinyi Wang,1 Huilin Zhao,1 Meilan Li,2 Fangyou Yu1 1Department of Clinical Laboratory, Shanghai Pulmonary Hospital, School of Medicine, Tongji University, Shanghai, 200433, People’s Republic of China; 2Respiratory Intensive Care Unit, Shanghai Pulmonary Hospital, School of Medicine, Tongji University, Shanghai, 200433, People’s Republic of ChinaCorrespondence: Meilan LiRespiratory Intensive Care Unit, Shanghai Pulmonary Hospital, School of Medicine, Tongji University, 3rd Floor, Building 2, No. 507 Zhengmin Road, Yangpu District, Shanghai, People’s Republic of ChinaEmail lml73@163.comFangyou YuDepartment of Clinical Laboratory, Shanghai Pulmonary Hospital, School of Medicine, Tongji University, 3rd Floor, Building 2, No. 507 Zhengmin Road, Yangpu District, Shanghai, People’s Republic of ChinaTel +86 13575440803Email wzjxyfy@163.comIntroduction: Methicillin-resistant Staphylococcus aureus (MRSA) infections have become a leading cause of severe infections in both healthcare and community settings. Mutations in the rpoB gene cause resistance to rifampin (RIFR), a critical antibiotic for the treatment of multidrug-resistant Staphylococcus aureus. The aim of this study was to detect the molecular characteristics of RIFR MRSA and analyze the rpoB gene mutations involved in RIF resistance.Methods: A total of 49 RIFR MRSA and 38 RIFS MRSA isolates collected from seven cities in China were analyzed by multilocus sequence typing, staphylococcus chromosomal cassette mec (SCCmec) typing, spa typing, and rpoB gene mutations.Results: ST239-III-t030 (35/49, 71.4%), the major clone in RIFR MRSA isolates; ST45-IV-t116 (16/38, 42.1%), the major clone in RIFS MRSA isolates with rpoB mutations. RIFR MRSA isolates were resistant to erythromycin, ciprofloxacin, tetracycline, gentamicin, and clindamycin. By contrast, RIFS MRSA isolates with rpoB mutation were more susceptible to ciprofloxacin, tetracycline, and gentamicin. Forty-three (87.8%) isolates present the mutational change H481N and L466S, conferring 128– 512 μg/mL RIF resistance. The four isolates with RIF MIC ≥ 1024 μg/mL had additional amino acid substitution: H481N, L466S, A473T (n=2); H481Y (n=2), associated with a high-level RIF resistance. Of 38 RIFS MRSA isolates, two mutations were observed, including H481N (n=37) and A477D (n=1).Conclusion: In conclusion, the predominant RIFR MRSA clones in China were ST239-III-t030. Molecular characteristics, antibiotic-resistant profiles, and rpoB mutations between RIFR MRSA and RIFS MRSA were diverse. Antibiotics for treating patients with MRSA infections can be selected based on molecular characteristics.Keywords: MRSA, rifampin, rpoB mutations, MLST, SCCmec, spaGuo YWang BRao LWang XZhao HLi MYu FDove Medical Pressarticlemrsarifampinrpob mutationsmlstsccmecspa.Infectious and parasitic diseasesRC109-216ENInfection and Drug Resistance, Vol Volume 14, Pp 4591-4600 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	mrsa rifampin rpob mutations mlst sccmec spa. Infectious and parasitic diseases RC109-216
spellingShingle	mrsa rifampin rpob mutations mlst sccmec spa. Infectious and parasitic diseases RC109-216 Guo Y Wang B Rao L Wang X Zhao H Li M Yu F Molecular Characteristics of Rifampin-Sensitive and -Resistant Isolates and Characteristics of rpoB Gene Mutations in Methicillin-Resistant Staphylococcus aureus
description	Yinjuan Guo,1 Bingjie Wang,1 Lulin Rao,1 Xinyi Wang,1 Huilin Zhao,1 Meilan Li,2 Fangyou Yu1 1Department of Clinical Laboratory, Shanghai Pulmonary Hospital, School of Medicine, Tongji University, Shanghai, 200433, People’s Republic of China; 2Respiratory Intensive Care Unit, Shanghai Pulmonary Hospital, School of Medicine, Tongji University, Shanghai, 200433, People’s Republic of ChinaCorrespondence: Meilan LiRespiratory Intensive Care Unit, Shanghai Pulmonary Hospital, School of Medicine, Tongji University, 3rd Floor, Building 2, No. 507 Zhengmin Road, Yangpu District, Shanghai, People’s Republic of ChinaEmail lml73@163.comFangyou YuDepartment of Clinical Laboratory, Shanghai Pulmonary Hospital, School of Medicine, Tongji University, 3rd Floor, Building 2, No. 507 Zhengmin Road, Yangpu District, Shanghai, People’s Republic of ChinaTel +86 13575440803Email wzjxyfy@163.comIntroduction: Methicillin-resistant Staphylococcus aureus (MRSA) infections have become a leading cause of severe infections in both healthcare and community settings. Mutations in the rpoB gene cause resistance to rifampin (RIFR), a critical antibiotic for the treatment of multidrug-resistant Staphylococcus aureus. The aim of this study was to detect the molecular characteristics of RIFR MRSA and analyze the rpoB gene mutations involved in RIF resistance.Methods: A total of 49 RIFR MRSA and 38 RIFS MRSA isolates collected from seven cities in China were analyzed by multilocus sequence typing, staphylococcus chromosomal cassette mec (SCCmec) typing, spa typing, and rpoB gene mutations.Results: ST239-III-t030 (35/49, 71.4%), the major clone in RIFR MRSA isolates; ST45-IV-t116 (16/38, 42.1%), the major clone in RIFS MRSA isolates with rpoB mutations. RIFR MRSA isolates were resistant to erythromycin, ciprofloxacin, tetracycline, gentamicin, and clindamycin. By contrast, RIFS MRSA isolates with rpoB mutation were more susceptible to ciprofloxacin, tetracycline, and gentamicin. Forty-three (87.8%) isolates present the mutational change H481N and L466S, conferring 128– 512 μg/mL RIF resistance. The four isolates with RIF MIC ≥ 1024 μg/mL had additional amino acid substitution: H481N, L466S, A473T (n=2); H481Y (n=2), associated with a high-level RIF resistance. Of 38 RIFS MRSA isolates, two mutations were observed, including H481N (n=37) and A477D (n=1).Conclusion: In conclusion, the predominant RIFR MRSA clones in China were ST239-III-t030. Molecular characteristics, antibiotic-resistant profiles, and rpoB mutations between RIFR MRSA and RIFS MRSA were diverse. Antibiotics for treating patients with MRSA infections can be selected based on molecular characteristics.Keywords: MRSA, rifampin, rpoB mutations, MLST, SCCmec, spa
format	article
author	Guo Y Wang B Rao L Wang X Zhao H Li M Yu F
author_facet	Guo Y Wang B Rao L Wang X Zhao H Li M Yu F
author_sort	Guo Y
title	Molecular Characteristics of Rifampin-Sensitive and -Resistant Isolates and Characteristics of rpoB Gene Mutations in Methicillin-Resistant Staphylococcus aureus
title_short	Molecular Characteristics of Rifampin-Sensitive and -Resistant Isolates and Characteristics of rpoB Gene Mutations in Methicillin-Resistant Staphylococcus aureus
title_full	Molecular Characteristics of Rifampin-Sensitive and -Resistant Isolates and Characteristics of rpoB Gene Mutations in Methicillin-Resistant Staphylococcus aureus
title_fullStr	Molecular Characteristics of Rifampin-Sensitive and -Resistant Isolates and Characteristics of rpoB Gene Mutations in Methicillin-Resistant Staphylococcus aureus
title_full_unstemmed	Molecular Characteristics of Rifampin-Sensitive and -Resistant Isolates and Characteristics of rpoB Gene Mutations in Methicillin-Resistant Staphylococcus aureus
title_sort	molecular characteristics of rifampin-sensitive and -resistant isolates and characteristics of rpob gene mutations in methicillin-resistant staphylococcus aureus
publisher	Dove Medical Press
publishDate	2021
url	https://doaj.org/article/388afe49e0a14095b67b6c44911f5993
work_keys_str_mv	AT guoy molecularcharacteristicsofrifampinsensitiveandresistantisolatesandcharacteristicsofrpobgenemutationsinmethicillinresistantstaphylococcusaureus AT wangb molecularcharacteristicsofrifampinsensitiveandresistantisolatesandcharacteristicsofrpobgenemutationsinmethicillinresistantstaphylococcusaureus AT raol molecularcharacteristicsofrifampinsensitiveandresistantisolatesandcharacteristicsofrpobgenemutationsinmethicillinresistantstaphylococcusaureus AT wangx molecularcharacteristicsofrifampinsensitiveandresistantisolatesandcharacteristicsofrpobgenemutationsinmethicillinresistantstaphylococcusaureus AT zhaoh molecularcharacteristicsofrifampinsensitiveandresistantisolatesandcharacteristicsofrpobgenemutationsinmethicillinresistantstaphylococcusaureus AT lim molecularcharacteristicsofrifampinsensitiveandresistantisolatesandcharacteristicsofrpobgenemutationsinmethicillinresistantstaphylococcusaureus AT yuf molecularcharacteristicsofrifampinsensitiveandresistantisolatesandcharacteristicsofrpobgenemutationsinmethicillinresistantstaphylococcusaureus
_version_	1718444641682980864

Molecular Characteristics of Rifampin-Sensitive and -Resistant Isolates and Characteristics of rpoB Gene Mutations in Methicillin-Resistant Staphylococcus aureus

Ejemplares similares