Probing the cytoadherence of malaria infected red blood cells under flow.

Malaria is one of the most widespread and deadly human parasitic diseases caused by the Plasmodium (P.) species with the P. falciparum being the most deadly. The parasites are capable of invading red blood cells (RBCs) during infection. At the late stage of parasites' development, the parasites...

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Autores principales:	Xiaofeng Xu, Artem K Efremov, Ang Li, Lipeng Lai, Ming Dao, Chwee Teck Lim, Jianshu Cao
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/388bb6e05221456293ac881b8f48c085
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spelling	oai:doaj.org-article:388bb6e05221456293ac881b8f48c0852021-11-18T07:44:04ZProbing the cytoadherence of malaria infected red blood cells under flow.1932-620310.1371/journal.pone.0064763https://doaj.org/article/388bb6e05221456293ac881b8f48c0852013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23724092/?tool=EBIhttps://doaj.org/toc/1932-6203Malaria is one of the most widespread and deadly human parasitic diseases caused by the Plasmodium (P.) species with the P. falciparum being the most deadly. The parasites are capable of invading red blood cells (RBCs) during infection. At the late stage of parasites' development, the parasites export proteins to the infected RBCs (iRBC) membrane and bind to receptors of surface proteins on the endothelial cells that line microvasculature walls. Resulting adhesion of iRBCs to microvasculature is one of the main sources of most complications during malaria infection. Therefore, it is important to develop a versatile and simple experimental method to quantitatively investigate iRBCs cytoadhesion and binding kinetics. Here, we developed an advanced flow based adhesion assay to demonstrate that iRBC's adhesion to endothelial CD36 receptor protein coated channels is a bistable process possessing a hysteresis loop. This finding confirms a recently developed model of cell adhesion which we used to fit our experimental data. We measured the contact area of iRBC under shear flow at different stages of infection using Total Internal Reflection Fluorescence (TIRF), and also adhesion receptor and ligand binding kinetics using Atomic Force Microscopy (AFM). With these parameters, we reproduced in our model the experimentally observed changes in adhesion properties of iRBCs accompanying parasite maturation and investigated the main mechanisms responsible for these changes, which are the contact area during the shear flow as well as the rupture area size.Xiaofeng XuArtem K EfremovAng LiLipeng LaiMing DaoChwee Teck LimJianshu CaoPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 5, p e64763 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Xiaofeng Xu Artem K Efremov Ang Li Lipeng Lai Ming Dao Chwee Teck Lim Jianshu Cao Probing the cytoadherence of malaria infected red blood cells under flow.
description	Malaria is one of the most widespread and deadly human parasitic diseases caused by the Plasmodium (P.) species with the P. falciparum being the most deadly. The parasites are capable of invading red blood cells (RBCs) during infection. At the late stage of parasites' development, the parasites export proteins to the infected RBCs (iRBC) membrane and bind to receptors of surface proteins on the endothelial cells that line microvasculature walls. Resulting adhesion of iRBCs to microvasculature is one of the main sources of most complications during malaria infection. Therefore, it is important to develop a versatile and simple experimental method to quantitatively investigate iRBCs cytoadhesion and binding kinetics. Here, we developed an advanced flow based adhesion assay to demonstrate that iRBC's adhesion to endothelial CD36 receptor protein coated channels is a bistable process possessing a hysteresis loop. This finding confirms a recently developed model of cell adhesion which we used to fit our experimental data. We measured the contact area of iRBC under shear flow at different stages of infection using Total Internal Reflection Fluorescence (TIRF), and also adhesion receptor and ligand binding kinetics using Atomic Force Microscopy (AFM). With these parameters, we reproduced in our model the experimentally observed changes in adhesion properties of iRBCs accompanying parasite maturation and investigated the main mechanisms responsible for these changes, which are the contact area during the shear flow as well as the rupture area size.
format	article
author	Xiaofeng Xu Artem K Efremov Ang Li Lipeng Lai Ming Dao Chwee Teck Lim Jianshu Cao
author_facet	Xiaofeng Xu Artem K Efremov Ang Li Lipeng Lai Ming Dao Chwee Teck Lim Jianshu Cao
author_sort	Xiaofeng Xu
title	Probing the cytoadherence of malaria infected red blood cells under flow.
title_short	Probing the cytoadherence of malaria infected red blood cells under flow.
title_full	Probing the cytoadherence of malaria infected red blood cells under flow.
title_fullStr	Probing the cytoadherence of malaria infected red blood cells under flow.
title_full_unstemmed	Probing the cytoadherence of malaria infected red blood cells under flow.
title_sort	probing the cytoadherence of malaria infected red blood cells under flow.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/388bb6e05221456293ac881b8f48c085
work_keys_str_mv	AT xiaofengxu probingthecytoadherenceofmalariainfectedredbloodcellsunderflow AT artemkefremov probingthecytoadherenceofmalariainfectedredbloodcellsunderflow AT angli probingthecytoadherenceofmalariainfectedredbloodcellsunderflow AT lipenglai probingthecytoadherenceofmalariainfectedredbloodcellsunderflow AT mingdao probingthecytoadherenceofmalariainfectedredbloodcellsunderflow AT chweetecklim probingthecytoadherenceofmalariainfectedredbloodcellsunderflow AT jianshucao probingthecytoadherenceofmalariainfectedredbloodcellsunderflow
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Probing the cytoadherence of malaria infected red blood cells under flow.

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