FREQUENCY OF LOSS OF SUCCINATE DEHYDROGENASE-B IN GASTROINTESTINAL STROMAL TUMORS: SHAUKAT KHANUM HOSPITAL EXPERIENCE

Objective: To assess the frequency of loss of expression of succinate dehydrogenase-B (SDH-B) in gastrointestinal stromal tumors (GISTS) of our population through immunohistochemistry. Study Design: Descriptive study. Place and Duration of Study: Department of Pathology, Shaukat Khanum Hospital &a...

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Autores principales:	Dr Syed Saad Gardezi, Asif Loya, Noreen Akhter, Sajid Mushtaq, Usman Hassan, Zain Mehdi, Omer Waqas
Formato:	article
Lenguaje:	EN
Publicado:	Army Medical College Rawalpindi 2018
Materias:	sdh-b immunohistochemistry Medicine R Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/38923565ad5e4388a035221ad29ff601
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spelling	oai:doaj.org-article:38923565ad5e4388a035221ad29ff6012021-12-03T07:13:46ZFREQUENCY OF LOSS OF SUCCINATE DEHYDROGENASE-B IN GASTROINTESTINAL STROMAL TUMORS: SHAUKAT KHANUM HOSPITAL EXPERIENCE0030-96482411-8842https://doaj.org/article/38923565ad5e4388a035221ad29ff6012018-08-01T00:00:00Zhttps://www.pafmj.org/index.php/PAFMJ/article/view/4/1https://doaj.org/toc/0030-9648https://doaj.org/toc/2411-8842Objective: To assess the frequency of loss of expression of succinate dehydrogenase-B (SDH-B) in gastrointestinal stromal tumors (GISTS) of our population through immunohistochemistry. Study Design: Descriptive study. Place and Duration of Study: Department of Pathology, Shaukat Khanum Hospital & Research Centre, Lahore for a duration of 6 months, Apr 2015 to Sep 2015. Patients and Methods: A total of 63 diagnosed cases of GISTS suitable by inclusion criteria were included in the study. The blocks of already diagnosed cases was reviewed by 2 pathologists and after confirmation SDH-B immunohistochemical stain was applied with normal tissue as its control. Lack of staining in tumor was interpreted as “Loss of expression”. Results: Mean age of our population was n=44.83 ± 17.76 years. The most common tumor site was small intestine n=23 (63.5%) cases, followed by stomach, n=15 cases. CD117 was positive in all the tumors. Only n=3 tumors were found to be SDH-B deficient. All of these tumors involved the stomach and had a median age of 20 years. All of these tumors had an epithelioid morphology. Conclusion: Immunohistochemistry was found reliable and cost-effective method for detecting mutation in SDH gene. Based on findings of this study it is recommended that all gastric GISTs below the age of 30 years with epithelioid morphology should be tested for SDH deficiency.Dr Syed Saad GardeziAsif LoyaNoreen AkhterSajid MushtaqUsman HassanZain MehdiOmer WaqasArmy Medical College Rawalpindiarticlesdh-bimmunohistochemistryMedicineRMedicine (General)R5-920ENPakistan Armed Forces Medical Journal, Vol 68, Iss 4, Pp 687-691 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	sdh-b immunohistochemistry Medicine R Medicine (General) R5-920
spellingShingle	sdh-b immunohistochemistry Medicine R Medicine (General) R5-920 Dr Syed Saad Gardezi Asif Loya Noreen Akhter Sajid Mushtaq Usman Hassan Zain Mehdi Omer Waqas FREQUENCY OF LOSS OF SUCCINATE DEHYDROGENASE-B IN GASTROINTESTINAL STROMAL TUMORS: SHAUKAT KHANUM HOSPITAL EXPERIENCE
description	Objective: To assess the frequency of loss of expression of succinate dehydrogenase-B (SDH-B) in gastrointestinal stromal tumors (GISTS) of our population through immunohistochemistry. Study Design: Descriptive study. Place and Duration of Study: Department of Pathology, Shaukat Khanum Hospital & Research Centre, Lahore for a duration of 6 months, Apr 2015 to Sep 2015. Patients and Methods: A total of 63 diagnosed cases of GISTS suitable by inclusion criteria were included in the study. The blocks of already diagnosed cases was reviewed by 2 pathologists and after confirmation SDH-B immunohistochemical stain was applied with normal tissue as its control. Lack of staining in tumor was interpreted as “Loss of expression”. Results: Mean age of our population was n=44.83 ± 17.76 years. The most common tumor site was small intestine n=23 (63.5%) cases, followed by stomach, n=15 cases. CD117 was positive in all the tumors. Only n=3 tumors were found to be SDH-B deficient. All of these tumors involved the stomach and had a median age of 20 years. All of these tumors had an epithelioid morphology. Conclusion: Immunohistochemistry was found reliable and cost-effective method for detecting mutation in SDH gene. Based on findings of this study it is recommended that all gastric GISTs below the age of 30 years with epithelioid morphology should be tested for SDH deficiency.
format	article
author	Dr Syed Saad Gardezi Asif Loya Noreen Akhter Sajid Mushtaq Usman Hassan Zain Mehdi Omer Waqas
author_facet	Dr Syed Saad Gardezi Asif Loya Noreen Akhter Sajid Mushtaq Usman Hassan Zain Mehdi Omer Waqas
author_sort	Dr Syed Saad Gardezi
title	FREQUENCY OF LOSS OF SUCCINATE DEHYDROGENASE-B IN GASTROINTESTINAL STROMAL TUMORS: SHAUKAT KHANUM HOSPITAL EXPERIENCE
title_short	FREQUENCY OF LOSS OF SUCCINATE DEHYDROGENASE-B IN GASTROINTESTINAL STROMAL TUMORS: SHAUKAT KHANUM HOSPITAL EXPERIENCE
title_full	FREQUENCY OF LOSS OF SUCCINATE DEHYDROGENASE-B IN GASTROINTESTINAL STROMAL TUMORS: SHAUKAT KHANUM HOSPITAL EXPERIENCE
title_fullStr	FREQUENCY OF LOSS OF SUCCINATE DEHYDROGENASE-B IN GASTROINTESTINAL STROMAL TUMORS: SHAUKAT KHANUM HOSPITAL EXPERIENCE
title_full_unstemmed	FREQUENCY OF LOSS OF SUCCINATE DEHYDROGENASE-B IN GASTROINTESTINAL STROMAL TUMORS: SHAUKAT KHANUM HOSPITAL EXPERIENCE
title_sort	frequency of loss of succinate dehydrogenase-b in gastrointestinal stromal tumors: shaukat khanum hospital experience
publisher	Army Medical College Rawalpindi
publishDate	2018
url	https://doaj.org/article/38923565ad5e4388a035221ad29ff601
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FREQUENCY OF LOSS OF SUCCINATE DEHYDROGENASE-B IN GASTROINTESTINAL STROMAL TUMORS: SHAUKAT KHANUM HOSPITAL EXPERIENCE

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