Biochemical adaptation in brain Acetylcholinesterase during acclimation to sub-lethal temperatures in the eurythermal fish Tilapia mossambica

Abstract Tilapia mossambica is a eurythermal tropical fish. We studied the effect of temperature on the kinetics of brain Acetylcholinesterase (AChE) during adaptation to sublethal temperatures by acclimating the fish to 37 °C, and controls to 25 °C. Electrophoresis showed the presence of two AChE b...

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Autores principales:	Vijay Aswani, David Trabucco
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2019
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/3893d2af8e7f4b68b58cde98cbe0f584
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spelling	oai:doaj.org-article:3893d2af8e7f4b68b58cde98cbe0f5842021-12-02T13:35:11ZBiochemical adaptation in brain Acetylcholinesterase during acclimation to sub-lethal temperatures in the eurythermal fish Tilapia mossambica10.1038/s41598-019-56066-x2045-2322https://doaj.org/article/3893d2af8e7f4b68b58cde98cbe0f5842019-12-01T00:00:00Zhttps://doi.org/10.1038/s41598-019-56066-xhttps://doaj.org/toc/2045-2322Abstract Tilapia mossambica is a eurythermal tropical fish. We studied the effect of temperature on the kinetics of brain Acetylcholinesterase (AChE) during adaptation to sublethal temperatures by acclimating the fish to 37 °C, and controls to 25 °C. Electrophoresis showed the presence of two AChE bands that did not change in position or intensity with acclimation period or temperature. The apparent Km was 0.23 ± 0.01 mM ATChI and remained relatively constant over the in vitro assay temperature range 10 °C to 40 °C. Biochemical characterization suggested that the enzyme is a ‘eurytolerant protein’ in its stability of kinetic and thermal properties over a wide temperature range. Thermal stability and arrhenius plots suggested that the AChE was made up of two forms that differed in their thermal properties.The two molecular forms of acetylcholinesterase were purified from the brain of T. mossambica. Molecular weight studies revealed that the two forms were size isomers: a monomer of 59 KDa and a tetramer of 244 KDa. They differed in their Kms, thermal stabilities and energies of activation. We suggest that biochemical adaptation to temperature in the brain acetylcholinerase system of the fish Tilapia mossambica is based on the aggregation-dissociation of these size isomers.Vijay AswaniDavid TrabuccoNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 9, Iss 1, Pp 1-11 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Vijay Aswani David Trabucco Biochemical adaptation in brain Acetylcholinesterase during acclimation to sub-lethal temperatures in the eurythermal fish Tilapia mossambica
description	Abstract Tilapia mossambica is a eurythermal tropical fish. We studied the effect of temperature on the kinetics of brain Acetylcholinesterase (AChE) during adaptation to sublethal temperatures by acclimating the fish to 37 °C, and controls to 25 °C. Electrophoresis showed the presence of two AChE bands that did not change in position or intensity with acclimation period or temperature. The apparent Km was 0.23 ± 0.01 mM ATChI and remained relatively constant over the in vitro assay temperature range 10 °C to 40 °C. Biochemical characterization suggested that the enzyme is a ‘eurytolerant protein’ in its stability of kinetic and thermal properties over a wide temperature range. Thermal stability and arrhenius plots suggested that the AChE was made up of two forms that differed in their thermal properties.The two molecular forms of acetylcholinesterase were purified from the brain of T. mossambica. Molecular weight studies revealed that the two forms were size isomers: a monomer of 59 KDa and a tetramer of 244 KDa. They differed in their Kms, thermal stabilities and energies of activation. We suggest that biochemical adaptation to temperature in the brain acetylcholinerase system of the fish Tilapia mossambica is based on the aggregation-dissociation of these size isomers.
format	article
author	Vijay Aswani David Trabucco
author_facet	Vijay Aswani David Trabucco
author_sort	Vijay Aswani
title	Biochemical adaptation in brain Acetylcholinesterase during acclimation to sub-lethal temperatures in the eurythermal fish Tilapia mossambica
title_short	Biochemical adaptation in brain Acetylcholinesterase during acclimation to sub-lethal temperatures in the eurythermal fish Tilapia mossambica
title_full	Biochemical adaptation in brain Acetylcholinesterase during acclimation to sub-lethal temperatures in the eurythermal fish Tilapia mossambica
title_fullStr	Biochemical adaptation in brain Acetylcholinesterase during acclimation to sub-lethal temperatures in the eurythermal fish Tilapia mossambica
title_full_unstemmed	Biochemical adaptation in brain Acetylcholinesterase during acclimation to sub-lethal temperatures in the eurythermal fish Tilapia mossambica
title_sort	biochemical adaptation in brain acetylcholinesterase during acclimation to sub-lethal temperatures in the eurythermal fish tilapia mossambica
publisher	Nature Portfolio
publishDate	2019
url	https://doaj.org/article/3893d2af8e7f4b68b58cde98cbe0f584
work_keys_str_mv	AT vijayaswani biochemicaladaptationinbrainacetylcholinesteraseduringacclimationtosublethaltemperaturesintheeurythermalfishtilapiamossambica AT davidtrabucco biochemicaladaptationinbrainacetylcholinesteraseduringacclimationtosublethaltemperaturesintheeurythermalfishtilapiamossambica
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Biochemical adaptation in brain Acetylcholinesterase during acclimation to sub-lethal temperatures in the eurythermal fish Tilapia mossambica

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