In vitro placental model optimization for nanoparticle transport studies

Laura Cartwright1, Marie S&oslash;nnegaard Poulsen2, Hanne M&oslash;rck Nielsen3, Giulio Pojana4, Lisbeth E Knudsen2, Margaret Saunders1, Erik Rytting2,51Bristol Initiative for Research of Child Health (BIRCH), Biophysics Research Unit, St Michael&#39;s Hospital, UH Bristol N...

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Autores principales:	Cartwright L, Poulsen MS, Nielsen HM, Pojana G, Knudsen LE, Saunders M, Rytting E
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2012
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/3895caba51b3417abce4fbed8b1358ae
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spelling	oai:doaj.org-article:3895caba51b3417abce4fbed8b1358ae2021-12-02T07:20:21ZIn vitro placental model optimization for nanoparticle transport studies1176-91141178-2013https://doaj.org/article/3895caba51b3417abce4fbed8b1358ae2012-01-01T00:00:00Zhttp://www.dovepress.com/in-vitro-placental-model-optimization-for-nanoparticle-transport-studi-a9157https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Laura Cartwright1, Marie S&oslash;nnegaard Poulsen2, Hanne M&oslash;rck Nielsen3, Giulio Pojana4, Lisbeth E Knudsen2, Margaret Saunders1, Erik Rytting2,51Bristol Initiative for Research of Child Health (BIRCH), Biophysics Research Unit, St Michael&#39;s Hospital, UH Bristol NHS Foundation Trust, Bristol, UK; 2University of Copenhagen, Faculty of Health Sciences, Department of Public Health, 3University of Copenhagen, Faculty of Pharmaceutical Sciences, Department of Pharmaceutics and Analytical Chemistry, Copenhagen, Denmark; 4Department of Environmental Sciences, Informatics and Statistics, University Ca&#39; Foscari Venice, Venice, Italy; 5Department of Obstetrics and Gynecology, University of Texas Medical Branch, Galveston, Texas, USABackground: Advances in biomedical nanotechnology raise hopes in patient populations but may also raise questions regarding biodistribution and biocompatibility, especially during pregnancy. Special consideration must be given to the placenta as a biological barrier because a pregnant woman&#39;s exposure to nanoparticles could have significant effects on the fetus developing in the womb. Therefore, the purpose of this study is to optimize an in vitro model for characterizing the transport of nanoparticles across human placental trophoblast cells.Methods: The growth of BeWo (clone b30) human placental choriocarcinoma cells for nanoparticle transport studies was characterized in terms of optimized Transwell&reg; insert type and pore size, the investigation of barrier properties by transmission electron microscopy, tight junction staining, transepithelial electrical resistance, and fluorescein sodium transport. Following the determination of nontoxic concentrations of fluorescent polystyrene nanoparticles, the cellular uptake and transport of 50 nm and 100 nm diameter particles was measured using the in vitro BeWo cell model.Results: Particle size measurements, fluorescence readings, and confocal microscopy indicated both cellular uptake of the fluorescent polystyrene nanoparticles and the transcellular transport of these particles from the apical (maternal) to the basolateral (fetal) compartment. Over the course of 24 hours, the apparent permeability across BeWo cells grown on polycarbonate membranes (3.0 &micro;m pore size) was four times higher for the 50 nm particles compared with the 100 nm particles.Conclusion: The BeWo cell line has been optimized and shown to be a valid in vitro model for studying the transplacental transport of nanoparticles. Fluorescent polystyrene nanoparticle transport was size-dependent, as smaller particles reached the basal (fetal) compartment at a higher rate.Keywords: nanoparticles, placenta, BeWo cells, transport, model optimization, nanotoxicologyCartwright LPoulsen MSNielsen HMPojana GKnudsen LESaunders MRytting EDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2012, Iss default, Pp 497-510 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Cartwright L Poulsen MS Nielsen HM Pojana G Knudsen LE Saunders M Rytting E In vitro placental model optimization for nanoparticle transport studies
description	Laura Cartwright1, Marie S&oslash;nnegaard Poulsen2, Hanne M&oslash;rck Nielsen3, Giulio Pojana4, Lisbeth E Knudsen2, Margaret Saunders1, Erik Rytting2,51Bristol Initiative for Research of Child Health (BIRCH), Biophysics Research Unit, St Michael&#39;s Hospital, UH Bristol NHS Foundation Trust, Bristol, UK; 2University of Copenhagen, Faculty of Health Sciences, Department of Public Health, 3University of Copenhagen, Faculty of Pharmaceutical Sciences, Department of Pharmaceutics and Analytical Chemistry, Copenhagen, Denmark; 4Department of Environmental Sciences, Informatics and Statistics, University Ca&#39; Foscari Venice, Venice, Italy; 5Department of Obstetrics and Gynecology, University of Texas Medical Branch, Galveston, Texas, USABackground: Advances in biomedical nanotechnology raise hopes in patient populations but may also raise questions regarding biodistribution and biocompatibility, especially during pregnancy. Special consideration must be given to the placenta as a biological barrier because a pregnant woman&#39;s exposure to nanoparticles could have significant effects on the fetus developing in the womb. Therefore, the purpose of this study is to optimize an in vitro model for characterizing the transport of nanoparticles across human placental trophoblast cells.Methods: The growth of BeWo (clone b30) human placental choriocarcinoma cells for nanoparticle transport studies was characterized in terms of optimized Transwell&reg; insert type and pore size, the investigation of barrier properties by transmission electron microscopy, tight junction staining, transepithelial electrical resistance, and fluorescein sodium transport. Following the determination of nontoxic concentrations of fluorescent polystyrene nanoparticles, the cellular uptake and transport of 50 nm and 100 nm diameter particles was measured using the in vitro BeWo cell model.Results: Particle size measurements, fluorescence readings, and confocal microscopy indicated both cellular uptake of the fluorescent polystyrene nanoparticles and the transcellular transport of these particles from the apical (maternal) to the basolateral (fetal) compartment. Over the course of 24 hours, the apparent permeability across BeWo cells grown on polycarbonate membranes (3.0 &micro;m pore size) was four times higher for the 50 nm particles compared with the 100 nm particles.Conclusion: The BeWo cell line has been optimized and shown to be a valid in vitro model for studying the transplacental transport of nanoparticles. Fluorescent polystyrene nanoparticle transport was size-dependent, as smaller particles reached the basal (fetal) compartment at a higher rate.Keywords: nanoparticles, placenta, BeWo cells, transport, model optimization, nanotoxicology
format	article
author	Cartwright L Poulsen MS Nielsen HM Pojana G Knudsen LE Saunders M Rytting E
author_facet	Cartwright L Poulsen MS Nielsen HM Pojana G Knudsen LE Saunders M Rytting E
author_sort	Cartwright L
title	In vitro placental model optimization for nanoparticle transport studies
title_short	In vitro placental model optimization for nanoparticle transport studies
title_full	In vitro placental model optimization for nanoparticle transport studies
title_fullStr	In vitro placental model optimization for nanoparticle transport studies
title_full_unstemmed	In vitro placental model optimization for nanoparticle transport studies
title_sort	in vitro placental model optimization for nanoparticle transport studies
publisher	Dove Medical Press
publishDate	2012
url	https://doaj.org/article/3895caba51b3417abce4fbed8b1358ae
work_keys_str_mv	AT cartwrightl invitroplacentalmodeloptimizationfornanoparticletransportstudies AT poulsenms invitroplacentalmodeloptimizationfornanoparticletransportstudies AT nielsenhm invitroplacentalmodeloptimizationfornanoparticletransportstudies AT pojanag invitroplacentalmodeloptimizationfornanoparticletransportstudies AT knudsenle invitroplacentalmodeloptimizationfornanoparticletransportstudies AT saundersm invitroplacentalmodeloptimizationfornanoparticletransportstudies AT ryttinge invitroplacentalmodeloptimizationfornanoparticletransportstudies
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In vitro placental model optimization for nanoparticle transport studies

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