In vitro Anti-inflammatory Effects of Satureja Kkhuzestanica Essential Oil Compared to Carvacrol

Introduction: Satureja khozestanica grows mainly in the southwest part of Iran as a native plant. This edible herb contains various compounds including the S. Khuzestanica essential oil (SKEO) and monoterpene known as Carvacrol. Previous studies have shown the anti-inflammatory effects of S. Khuzest...

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Autores principales:	Masumeh Jalalvand, Gholamreza Shahsavari, Ali Sheikhian, Ali Ganji, Ghasem Mosayebi
Formato:	article
Lenguaje:	EN
Publicado:	Zabol University of Medical sciences 2020
Materias:	carvacrol satureja khuzestanica cyclooxygenase-2 gene Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/389d5160acfd48178554216cde786e3f
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spelling	oai:doaj.org-article:389d5160acfd48178554216cde786e3f2021-11-17T09:54:25ZIn vitro Anti-inflammatory Effects of Satureja Kkhuzestanica Essential Oil Compared to Carvacrol2476-664X10.34172/ijbsm.2020.12https://doaj.org/article/389d5160acfd48178554216cde786e3f2020-06-01T00:00:00Zhttp://ijbsm.zbmu.ac.ir/PDF/ijbsm-14403https://doaj.org/toc/2476-664XIntroduction: Satureja khozestanica grows mainly in the southwest part of Iran as a native plant. This edible herb contains various compounds including the S. Khuzestanica essential oil (SKEO) and monoterpene known as Carvacrol. Previous studies have shown the anti-inflammatory effects of S. Khuzestanica without mentioning the exact mechanism of its function. Given that prostaglandin synthesis, which is one of the main mediators of inflammation, is regulated by the cyclooxygenase-2 (COX2) gene, the present study investigated the effects of SKEO and Carvacrol on the expression of the COX2 gene in the stimulated-J774A.1 macrophage cell line. Methods: To this end, fresh aerial parts of the plant were processed to prepare SKEO. Then, different doses of SKEO and Carvacrol (i.e., 0.004%, 0.008%, and 0.016% v/v) were used to treat with the lipopolysaccharides (LPS)-stimulated cell line for eight hours. After RNA extraction, the real-time polymerase chain reaction technique was applied for gene expression analysis. Results: In the LPS-stimulated J774A.1 macrophage cell line, COX2 gene expression reduced significantly in a dose-dependent manner (0.004%, 0.008%, and 0.016%, P = 0.024, P = 0.021, and P = 0.013 v/v of SKEO, respectively) by SKEO, and the effect of Carvacrol was less powerful (0.008% and 0.016%, P = 0.027 and P = 0.038 v/v, respectively) compared to SKEO. Finally, the comparison between SKEO and Carvacrol showed higher significant inhibitory effects of SKEO on COX2 gene expression in comparison with Carvacrol in 0.004% v/v concentration (P = 0.037). Conclusion: In general, SKEO significantly reduced COX2 gene expression, thus it can be suggested that its anti-inflammatory effect may result from the inhibition of the synthesis of this pro-inflammatory gene.Masumeh JalalvandGholamreza ShahsavariAli SheikhianAli GanjiGhasem MosayebiZabol University of Medical sciencesarticlecarvacrol satureja khuzestanica cyclooxygenase-2 gene Medicine (General)R5-920ENInternational Journal of Basic Science in Medicine, Vol 5, Iss 2, Pp 61-65 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	carvacrol satureja khuzestanica cyclooxygenase-2 gene Medicine (General) R5-920
spellingShingle	carvacrol satureja khuzestanica cyclooxygenase-2 gene Medicine (General) R5-920 Masumeh Jalalvand Gholamreza Shahsavari Ali Sheikhian Ali Ganji Ghasem Mosayebi In vitro Anti-inflammatory Effects of Satureja Kkhuzestanica Essential Oil Compared to Carvacrol
description	Introduction: Satureja khozestanica grows mainly in the southwest part of Iran as a native plant. This edible herb contains various compounds including the S. Khuzestanica essential oil (SKEO) and monoterpene known as Carvacrol. Previous studies have shown the anti-inflammatory effects of S. Khuzestanica without mentioning the exact mechanism of its function. Given that prostaglandin synthesis, which is one of the main mediators of inflammation, is regulated by the cyclooxygenase-2 (COX2) gene, the present study investigated the effects of SKEO and Carvacrol on the expression of the COX2 gene in the stimulated-J774A.1 macrophage cell line. Methods: To this end, fresh aerial parts of the plant were processed to prepare SKEO. Then, different doses of SKEO and Carvacrol (i.e., 0.004%, 0.008%, and 0.016% v/v) were used to treat with the lipopolysaccharides (LPS)-stimulated cell line for eight hours. After RNA extraction, the real-time polymerase chain reaction technique was applied for gene expression analysis. Results: In the LPS-stimulated J774A.1 macrophage cell line, COX2 gene expression reduced significantly in a dose-dependent manner (0.004%, 0.008%, and 0.016%, P = 0.024, P = 0.021, and P = 0.013 v/v of SKEO, respectively) by SKEO, and the effect of Carvacrol was less powerful (0.008% and 0.016%, P = 0.027 and P = 0.038 v/v, respectively) compared to SKEO. Finally, the comparison between SKEO and Carvacrol showed higher significant inhibitory effects of SKEO on COX2 gene expression in comparison with Carvacrol in 0.004% v/v concentration (P = 0.037). Conclusion: In general, SKEO significantly reduced COX2 gene expression, thus it can be suggested that its anti-inflammatory effect may result from the inhibition of the synthesis of this pro-inflammatory gene.
format	article
author	Masumeh Jalalvand Gholamreza Shahsavari Ali Sheikhian Ali Ganji Ghasem Mosayebi
author_facet	Masumeh Jalalvand Gholamreza Shahsavari Ali Sheikhian Ali Ganji Ghasem Mosayebi
author_sort	Masumeh Jalalvand
title	In vitro Anti-inflammatory Effects of Satureja Kkhuzestanica Essential Oil Compared to Carvacrol
title_short	In vitro Anti-inflammatory Effects of Satureja Kkhuzestanica Essential Oil Compared to Carvacrol
title_full	In vitro Anti-inflammatory Effects of Satureja Kkhuzestanica Essential Oil Compared to Carvacrol
title_fullStr	In vitro Anti-inflammatory Effects of Satureja Kkhuzestanica Essential Oil Compared to Carvacrol
title_full_unstemmed	In vitro Anti-inflammatory Effects of Satureja Kkhuzestanica Essential Oil Compared to Carvacrol
title_sort	in vitro anti-inflammatory effects of satureja kkhuzestanica essential oil compared to carvacrol
publisher	Zabol University of Medical sciences
publishDate	2020
url	https://doaj.org/article/389d5160acfd48178554216cde786e3f
work_keys_str_mv	AT masumehjalalvand invitroantiinflammatoryeffectsofsaturejakkhuzestanicaessentialoilcomparedtocarvacrol AT gholamrezashahsavari invitroantiinflammatoryeffectsofsaturejakkhuzestanicaessentialoilcomparedtocarvacrol AT alisheikhian invitroantiinflammatoryeffectsofsaturejakkhuzestanicaessentialoilcomparedtocarvacrol AT aliganji invitroantiinflammatoryeffectsofsaturejakkhuzestanicaessentialoilcomparedtocarvacrol AT ghasemmosayebi invitroantiinflammatoryeffectsofsaturejakkhuzestanicaessentialoilcomparedtocarvacrol
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In vitro Anti-inflammatory Effects of Satureja Kkhuzestanica Essential Oil Compared to Carvacrol

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