A non-death role of the yeast metacaspase: Yca1p alters cell cycle dynamics.

Caspase proteases are a conserved protein family predominantly known for engaging and executing apoptotic cell death. Nevertheless, in higher eukaryotes, caspases also influence a variety of cell behaviors including differentiation, proliferation and growth control. S. cerevisiae expresses a primord...

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Autores principales:	Robin E C Lee, Lawrence G Puente, Mads Kaern, Lynn A Megeney
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2008
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/38a64e8a8597407e95bc1d33b08231a9
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spelling	oai:doaj.org-article:38a64e8a8597407e95bc1d33b08231a92021-11-25T06:11:10ZA non-death role of the yeast metacaspase: Yca1p alters cell cycle dynamics.1932-620310.1371/journal.pone.0002956https://doaj.org/article/38a64e8a8597407e95bc1d33b08231a92008-08-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/18698411/?tool=EBIhttps://doaj.org/toc/1932-6203Caspase proteases are a conserved protein family predominantly known for engaging and executing apoptotic cell death. Nevertheless, in higher eukaryotes, caspases also influence a variety of cell behaviors including differentiation, proliferation and growth control. S. cerevisiae expresses a primordial caspase, yca1, and exhibits apoptosis-like death under certain stresses; however, the benefit of a dedicated death program to single cell organisms is controversial. In the absence of a clear rationale to justify the evolutionary retention of a death only pathway, we hypothesize that yca1 also influences non-apoptotic events. We report that genetic ablation and/or catalytic inactivation of Yca1p leads to a longer G1/S transition accompanied by slower growth in fermentation conditions. Downregulation of Yca1p proteolytic activity also results in failure to arrest during nocodazole treatment, indicating that Yca1p participates in the G2/M mitotic checkpoint. 20s proteasome activity and ROS staining of the Delta yca1 strain is indistinguishable from its isogenic control suggesting that putative regulation of the oxidative stress response by Yca1p does not instigate the cell cycle phenotype. Our results demonstrate multiple non-death roles for yca1 in the cell cycle.Robin E C LeeLawrence G PuenteMads KaernLynn A MegeneyPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 3, Iss 8, p e2956 (2008)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Robin E C Lee Lawrence G Puente Mads Kaern Lynn A Megeney A non-death role of the yeast metacaspase: Yca1p alters cell cycle dynamics.
description	Caspase proteases are a conserved protein family predominantly known for engaging and executing apoptotic cell death. Nevertheless, in higher eukaryotes, caspases also influence a variety of cell behaviors including differentiation, proliferation and growth control. S. cerevisiae expresses a primordial caspase, yca1, and exhibits apoptosis-like death under certain stresses; however, the benefit of a dedicated death program to single cell organisms is controversial. In the absence of a clear rationale to justify the evolutionary retention of a death only pathway, we hypothesize that yca1 also influences non-apoptotic events. We report that genetic ablation and/or catalytic inactivation of Yca1p leads to a longer G1/S transition accompanied by slower growth in fermentation conditions. Downregulation of Yca1p proteolytic activity also results in failure to arrest during nocodazole treatment, indicating that Yca1p participates in the G2/M mitotic checkpoint. 20s proteasome activity and ROS staining of the Delta yca1 strain is indistinguishable from its isogenic control suggesting that putative regulation of the oxidative stress response by Yca1p does not instigate the cell cycle phenotype. Our results demonstrate multiple non-death roles for yca1 in the cell cycle.
format	article
author	Robin E C Lee Lawrence G Puente Mads Kaern Lynn A Megeney
author_facet	Robin E C Lee Lawrence G Puente Mads Kaern Lynn A Megeney
author_sort	Robin E C Lee
title	A non-death role of the yeast metacaspase: Yca1p alters cell cycle dynamics.
title_short	A non-death role of the yeast metacaspase: Yca1p alters cell cycle dynamics.
title_full	A non-death role of the yeast metacaspase: Yca1p alters cell cycle dynamics.
title_fullStr	A non-death role of the yeast metacaspase: Yca1p alters cell cycle dynamics.
title_full_unstemmed	A non-death role of the yeast metacaspase: Yca1p alters cell cycle dynamics.
title_sort	non-death role of the yeast metacaspase: yca1p alters cell cycle dynamics.
publisher	Public Library of Science (PLoS)
publishDate	2008
url	https://doaj.org/article/38a64e8a8597407e95bc1d33b08231a9
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A non-death role of the yeast metacaspase: Yca1p alters cell cycle dynamics.

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