Functionalized carbon nanotubes as suitable scaffold materials for proliferation and differentiation of canine mesenchymal stem cells

Kinsuk Das,1 AP Madhusoodan,1 Bhabesh Mili,1 Ajay Kumar,2 AC Saxena,3 Kuldeep Kumar,1 Mihir Sarkar,1 Praveen Singh,4 Sameer Srivastava,5 Sadhan Bag1 1Division of Physiology and Climatology, 2Biochemistry and Food Science Section, 3Division of Surgery, 4Biophysics, Electron Microscopy and Instrument...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Das K, Madhusoodan AP, Mili B, Kumar A, Saxena AC, Kumar K, Sarkar M, Singh P, Shrivastava S, Bag S
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2017
Materias:	Canine MSCs CNT films cellular behaviour cytocompatibility differentiation Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/38aa4c3236f643e5af6909cc75fb944c
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:38aa4c3236f643e5af6909cc75fb944c
record_format	dspace
spelling	oai:doaj.org-article:38aa4c3236f643e5af6909cc75fb944c2021-12-02T02:01:53ZFunctionalized carbon nanotubes as suitable scaffold materials for proliferation and differentiation of canine mesenchymal stem cells1178-2013https://doaj.org/article/38aa4c3236f643e5af6909cc75fb944c2017-04-01T00:00:00Zhttps://www.dovepress.com/functionalized-carbon-nanotubes-as-suitable-scaffold-materials-for-pro-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Kinsuk Das,1 AP Madhusoodan,1 Bhabesh Mili,1 Ajay Kumar,2 AC Saxena,3 Kuldeep Kumar,1 Mihir Sarkar,1 Praveen Singh,4 Sameer Srivastava,5 Sadhan Bag1 1Division of Physiology and Climatology, 2Biochemistry and Food Science Section, 3Division of Surgery, 4Biophysics, Electron Microscopy and Instrumentation Section, 5Division of Veterinary Biotechnology, Indian Council of Agricultural Research – Indian Veterinary Research Institute, Izatnagar, Bareilly, Uttar Pradesh, India Abstract: In the field of regenerative medicine, numerous potential applications of mesenchymal stem cells (MSCs) can be envisaged, due to their ability to differentiate into a range of tissues on the basis of the substrate on which they grow. With the advances in nanotechnology, carbon nanotubes (CNTs) have been widely explored for use as cell culture substrate in tissue engineering applications. In this study, canine bone marrow-derived MSCs were considered as the cellular model for an in vitro study to elucidate the collective cellular processes, using three different varieties of thin films of functionalized carbon nanotubes (COOH-single-walled CNTs [SWCNTs], COOH-multiwalled CNTs [MWCNTs] and polyethylene glycol [PEG]-SWCNTs), which were spray dried onto preheated cover slips. Cells spread out better on the CNT films, resulting in higher cell surface area and occurrence of filopodia, with parallel orientation of stress fiber bundles. Canine MSCs proliferated at a slower rate on all types of CNT substrates compared to the control, but no decline in cell number was noticed during the study period. Expression of apoptosis-associated genes decreased on the CNT substrates as time progressed. On flow cytometry after AnnexinV-fluorescein isothiocyanate/propidium iodide (PI) staining, total number of apoptotic and necrotic cells remained lower in COOH-functionalized films compared to PEG-functionalized ones. Collectively, these results indicate that COOH-MWCNT substrate provided an environment of low cytotoxicity. Canine MSCs were further induced to differentiate along osteogenic, chondrogenic, and neuronal lineages by culturing under specific differentiation conditions. The cytochemical and immunocytochemical staining results, as well as the expression of the bone marker genes, led us to hypothesize that the COOH-MWCNT substrate acted as a better cue, accelerating the osteogenic differentiation process. However, while chondrogenesis was promoted by COOH-SWCNT, neuronal differentiation was promoted by both COOH-SWNCT and COOH-MWCNT. Taken together, these findings suggest that COOH-functionalized CNTs represent a promising scaffold component for future utilization in the selective differentiation of canine MSCs in regenerative medicine. Keywords: canine MSCs, CNT films, cellular behavior, cytocompatibility, differentiationDas KMadhusoodan APMili BKumar ASaxena ACKumar KSarkar MSingh PShrivastava SBag SDove Medical PressarticleCanine MSCsCNT filmscellular behaviourcytocompatibilitydifferentiationMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 12, Pp 3235-3252 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Canine MSCs CNT films cellular behaviour cytocompatibility differentiation Medicine (General) R5-920
spellingShingle	Canine MSCs CNT films cellular behaviour cytocompatibility differentiation Medicine (General) R5-920 Das K Madhusoodan AP Mili B Kumar A Saxena AC Kumar K Sarkar M Singh P Shrivastava S Bag S Functionalized carbon nanotubes as suitable scaffold materials for proliferation and differentiation of canine mesenchymal stem cells
description	Kinsuk Das,1 AP Madhusoodan,1 Bhabesh Mili,1 Ajay Kumar,2 AC Saxena,3 Kuldeep Kumar,1 Mihir Sarkar,1 Praveen Singh,4 Sameer Srivastava,5 Sadhan Bag1 1Division of Physiology and Climatology, 2Biochemistry and Food Science Section, 3Division of Surgery, 4Biophysics, Electron Microscopy and Instrumentation Section, 5Division of Veterinary Biotechnology, Indian Council of Agricultural Research – Indian Veterinary Research Institute, Izatnagar, Bareilly, Uttar Pradesh, India Abstract: In the field of regenerative medicine, numerous potential applications of mesenchymal stem cells (MSCs) can be envisaged, due to their ability to differentiate into a range of tissues on the basis of the substrate on which they grow. With the advances in nanotechnology, carbon nanotubes (CNTs) have been widely explored for use as cell culture substrate in tissue engineering applications. In this study, canine bone marrow-derived MSCs were considered as the cellular model for an in vitro study to elucidate the collective cellular processes, using three different varieties of thin films of functionalized carbon nanotubes (COOH-single-walled CNTs [SWCNTs], COOH-multiwalled CNTs [MWCNTs] and polyethylene glycol [PEG]-SWCNTs), which were spray dried onto preheated cover slips. Cells spread out better on the CNT films, resulting in higher cell surface area and occurrence of filopodia, with parallel orientation of stress fiber bundles. Canine MSCs proliferated at a slower rate on all types of CNT substrates compared to the control, but no decline in cell number was noticed during the study period. Expression of apoptosis-associated genes decreased on the CNT substrates as time progressed. On flow cytometry after AnnexinV-fluorescein isothiocyanate/propidium iodide (PI) staining, total number of apoptotic and necrotic cells remained lower in COOH-functionalized films compared to PEG-functionalized ones. Collectively, these results indicate that COOH-MWCNT substrate provided an environment of low cytotoxicity. Canine MSCs were further induced to differentiate along osteogenic, chondrogenic, and neuronal lineages by culturing under specific differentiation conditions. The cytochemical and immunocytochemical staining results, as well as the expression of the bone marker genes, led us to hypothesize that the COOH-MWCNT substrate acted as a better cue, accelerating the osteogenic differentiation process. However, while chondrogenesis was promoted by COOH-SWCNT, neuronal differentiation was promoted by both COOH-SWNCT and COOH-MWCNT. Taken together, these findings suggest that COOH-functionalized CNTs represent a promising scaffold component for future utilization in the selective differentiation of canine MSCs in regenerative medicine. Keywords: canine MSCs, CNT films, cellular behavior, cytocompatibility, differentiation
format	article
author	Das K Madhusoodan AP Mili B Kumar A Saxena AC Kumar K Sarkar M Singh P Shrivastava S Bag S
author_facet	Das K Madhusoodan AP Mili B Kumar A Saxena AC Kumar K Sarkar M Singh P Shrivastava S Bag S
author_sort	Das K
title	Functionalized carbon nanotubes as suitable scaffold materials for proliferation and differentiation of canine mesenchymal stem cells
title_short	Functionalized carbon nanotubes as suitable scaffold materials for proliferation and differentiation of canine mesenchymal stem cells
title_full	Functionalized carbon nanotubes as suitable scaffold materials for proliferation and differentiation of canine mesenchymal stem cells
title_fullStr	Functionalized carbon nanotubes as suitable scaffold materials for proliferation and differentiation of canine mesenchymal stem cells
title_full_unstemmed	Functionalized carbon nanotubes as suitable scaffold materials for proliferation and differentiation of canine mesenchymal stem cells
title_sort	functionalized carbon nanotubes as suitable scaffold materials for proliferation and differentiation of canine mesenchymal stem cells
publisher	Dove Medical Press
publishDate	2017
url	https://doaj.org/article/38aa4c3236f643e5af6909cc75fb944c
work_keys_str_mv	AT dask functionalizedcarbonnanotubesassuitablescaffoldmaterialsforproliferationanddifferentiationofcaninemesenchymalstemcells AT madhusoodanap functionalizedcarbonnanotubesassuitablescaffoldmaterialsforproliferationanddifferentiationofcaninemesenchymalstemcells AT milib functionalizedcarbonnanotubesassuitablescaffoldmaterialsforproliferationanddifferentiationofcaninemesenchymalstemcells AT kumara functionalizedcarbonnanotubesassuitablescaffoldmaterialsforproliferationanddifferentiationofcaninemesenchymalstemcells AT saxenaac functionalizedcarbonnanotubesassuitablescaffoldmaterialsforproliferationanddifferentiationofcaninemesenchymalstemcells AT kumark functionalizedcarbonnanotubesassuitablescaffoldmaterialsforproliferationanddifferentiationofcaninemesenchymalstemcells AT sarkarm functionalizedcarbonnanotubesassuitablescaffoldmaterialsforproliferationanddifferentiationofcaninemesenchymalstemcells AT singhp functionalizedcarbonnanotubesassuitablescaffoldmaterialsforproliferationanddifferentiationofcaninemesenchymalstemcells AT shrivastavas functionalizedcarbonnanotubesassuitablescaffoldmaterialsforproliferationanddifferentiationofcaninemesenchymalstemcells AT bags functionalizedcarbonnanotubesassuitablescaffoldmaterialsforproliferationanddifferentiationofcaninemesenchymalstemcells
_version_	1718402768344973312

Functionalized carbon nanotubes as suitable scaffold materials for proliferation and differentiation of canine mesenchymal stem cells

Ejemplares similares