Coupling optogenetic stimulation with NanoLuc-based luminescence (BRET) Ca++ sensing

The coupling of optogenetics with fluorescent Ca2+ sensors is confounded by sensitivity of optogenetic probes to light used to excite the sensors. Here the authors develop a Ca2+ sensor based on bioluminescence resonance energy transfer (BRET) that monitors Ca2+fluxes in darkness without excitation.

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Autores principales:	Jie Yang, Derrick Cumberbatch, Samuel Centanni, Shu-qun Shi, Danny Winder, Donna Webb, Carl Hirschie Johnson
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2016
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/38ae3b777e7041d28342246b480b0c17
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spelling	oai:doaj.org-article:38ae3b777e7041d28342246b480b0c172021-12-02T15:35:26ZCoupling optogenetic stimulation with NanoLuc-based luminescence (BRET) Ca++ sensing10.1038/ncomms132682041-1723https://doaj.org/article/38ae3b777e7041d28342246b480b0c172016-10-01T00:00:00Zhttps://doi.org/10.1038/ncomms13268https://doaj.org/toc/2041-1723The coupling of optogenetics with fluorescent Ca2+ sensors is confounded by sensitivity of optogenetic probes to light used to excite the sensors. Here the authors develop a Ca2+ sensor based on bioluminescence resonance energy transfer (BRET) that monitors Ca2+fluxes in darkness without excitation.Jie YangDerrick CumberbatchSamuel CentanniShu-qun ShiDanny WinderDonna WebbCarl Hirschie JohnsonNature PortfolioarticleScienceQENNature Communications, Vol 7, Iss 1, Pp 1-10 (2016)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Jie Yang Derrick Cumberbatch Samuel Centanni Shu-qun Shi Danny Winder Donna Webb Carl Hirschie Johnson Coupling optogenetic stimulation with NanoLuc-based luminescence (BRET) Ca++ sensing
description	The coupling of optogenetics with fluorescent Ca2+ sensors is confounded by sensitivity of optogenetic probes to light used to excite the sensors. Here the authors develop a Ca2+ sensor based on bioluminescence resonance energy transfer (BRET) that monitors Ca2+fluxes in darkness without excitation.
format	article
author	Jie Yang Derrick Cumberbatch Samuel Centanni Shu-qun Shi Danny Winder Donna Webb Carl Hirschie Johnson
author_facet	Jie Yang Derrick Cumberbatch Samuel Centanni Shu-qun Shi Danny Winder Donna Webb Carl Hirschie Johnson
author_sort	Jie Yang
title	Coupling optogenetic stimulation with NanoLuc-based luminescence (BRET) Ca++ sensing
title_short	Coupling optogenetic stimulation with NanoLuc-based luminescence (BRET) Ca++ sensing
title_full	Coupling optogenetic stimulation with NanoLuc-based luminescence (BRET) Ca++ sensing
title_fullStr	Coupling optogenetic stimulation with NanoLuc-based luminescence (BRET) Ca++ sensing
title_full_unstemmed	Coupling optogenetic stimulation with NanoLuc-based luminescence (BRET) Ca++ sensing
title_sort	coupling optogenetic stimulation with nanoluc-based luminescence (bret) ca++ sensing
publisher	Nature Portfolio
publishDate	2016
url	https://doaj.org/article/38ae3b777e7041d28342246b480b0c17
work_keys_str_mv	AT jieyang couplingoptogeneticstimulationwithnanolucbasedluminescencebretcasensing AT derrickcumberbatch couplingoptogeneticstimulationwithnanolucbasedluminescencebretcasensing AT samuelcentanni couplingoptogeneticstimulationwithnanolucbasedluminescencebretcasensing AT shuqunshi couplingoptogeneticstimulationwithnanolucbasedluminescencebretcasensing AT dannywinder couplingoptogeneticstimulationwithnanolucbasedluminescencebretcasensing AT donnawebb couplingoptogeneticstimulationwithnanolucbasedluminescencebretcasensing AT carlhirschiejohnson couplingoptogeneticstimulationwithnanolucbasedluminescencebretcasensing
_version_	1718386526211014656

Coupling optogenetic stimulation with NanoLuc-based luminescence (BRET) Ca++ sensing

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