Coupling optogenetic stimulation with NanoLuc-based luminescence (BRET) Ca++ sensing
The coupling of optogenetics with fluorescent Ca2+ sensors is confounded by sensitivity of optogenetic probes to light used to excite the sensors. Here the authors develop a Ca2+ sensor based on bioluminescence resonance energy transfer (BRET) that monitors Ca2+fluxes in darkness without excitation.
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| Main Authors: | , , , , , , |
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| Format: | article |
| Language: | EN |
| Published: |
Nature Portfolio
2016
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| Subjects: | |
| Online Access: | https://doaj.org/article/38ae3b777e7041d28342246b480b0c17 |
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