Recombinant human plasma phospholipid transfer protein (PLTP) to prevent bacterial growth and to treat sepsis
Abstract Although plasma phospholipid transfer protein (PLTP) has been mainly studied in the context of atherosclerosis, it shares homology with proteins involved in innate immunity. Here, we produced active recombinant human PLTP (rhPLTP) in the milk of new lines of transgenic rabbits. We successfu...
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2017
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oai:doaj.org-article:38bc086aee0d4b64bfb9adb2801c05a82021-12-02T15:05:28ZRecombinant human plasma phospholipid transfer protein (PLTP) to prevent bacterial growth and to treat sepsis10.1038/s41598-017-03285-92045-2322https://doaj.org/article/38bc086aee0d4b64bfb9adb2801c05a82017-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-03285-9https://doaj.org/toc/2045-2322Abstract Although plasma phospholipid transfer protein (PLTP) has been mainly studied in the context of atherosclerosis, it shares homology with proteins involved in innate immunity. Here, we produced active recombinant human PLTP (rhPLTP) in the milk of new lines of transgenic rabbits. We successfully used rhPLTP as an exogenous therapeutic protein to treat endotoxemia and sepsis. In mouse models with injections of purified lipopolysaccharides or with polymicrobial infection, we demonstrated that rhPLTP prevented bacterial growth and detoxified LPS. In further support of the antimicrobial effect of PLTP, PLTP-knocked out mice were found to be less able than wild-type mice to fight against sepsis. To our knowledge, the production of rhPLTP to counter infection and to reduce endotoxemia and its harmful consequences is reported here for the first time. This paves the way for a novel strategy to satisfy long-felt, but unmet needs to prevent and treat sepsis.Valérie DeckertStéphanie LemairePierre-Jean RipollJean-Paul Pais de BarrosJérôme LabbéCaroline Chabert-Le BorgneVéronique TurquoisGuillaume MaquartDelphine LaroseNicolas DesrocheFranck MénétrierNaig Le GuernLorène J. LebrunCatherine DesrumauxThomas GautierJacques GroberCharles ThomasDavid MassonLouis-Marie HoudebineLaurent LagrostNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-16 (2017) |
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Medicine R Science Q Valérie Deckert Stéphanie Lemaire Pierre-Jean Ripoll Jean-Paul Pais de Barros Jérôme Labbé Caroline Chabert-Le Borgne Véronique Turquois Guillaume Maquart Delphine Larose Nicolas Desroche Franck Ménétrier Naig Le Guern Lorène J. Lebrun Catherine Desrumaux Thomas Gautier Jacques Grober Charles Thomas David Masson Louis-Marie Houdebine Laurent Lagrost Recombinant human plasma phospholipid transfer protein (PLTP) to prevent bacterial growth and to treat sepsis |
| description |
Abstract Although plasma phospholipid transfer protein (PLTP) has been mainly studied in the context of atherosclerosis, it shares homology with proteins involved in innate immunity. Here, we produced active recombinant human PLTP (rhPLTP) in the milk of new lines of transgenic rabbits. We successfully used rhPLTP as an exogenous therapeutic protein to treat endotoxemia and sepsis. In mouse models with injections of purified lipopolysaccharides or with polymicrobial infection, we demonstrated that rhPLTP prevented bacterial growth and detoxified LPS. In further support of the antimicrobial effect of PLTP, PLTP-knocked out mice were found to be less able than wild-type mice to fight against sepsis. To our knowledge, the production of rhPLTP to counter infection and to reduce endotoxemia and its harmful consequences is reported here for the first time. This paves the way for a novel strategy to satisfy long-felt, but unmet needs to prevent and treat sepsis. |
| format |
article |
| author |
Valérie Deckert Stéphanie Lemaire Pierre-Jean Ripoll Jean-Paul Pais de Barros Jérôme Labbé Caroline Chabert-Le Borgne Véronique Turquois Guillaume Maquart Delphine Larose Nicolas Desroche Franck Ménétrier Naig Le Guern Lorène J. Lebrun Catherine Desrumaux Thomas Gautier Jacques Grober Charles Thomas David Masson Louis-Marie Houdebine Laurent Lagrost |
| author_facet |
Valérie Deckert Stéphanie Lemaire Pierre-Jean Ripoll Jean-Paul Pais de Barros Jérôme Labbé Caroline Chabert-Le Borgne Véronique Turquois Guillaume Maquart Delphine Larose Nicolas Desroche Franck Ménétrier Naig Le Guern Lorène J. Lebrun Catherine Desrumaux Thomas Gautier Jacques Grober Charles Thomas David Masson Louis-Marie Houdebine Laurent Lagrost |
| author_sort |
Valérie Deckert |
| title |
Recombinant human plasma phospholipid transfer protein (PLTP) to prevent bacterial growth and to treat sepsis |
| title_short |
Recombinant human plasma phospholipid transfer protein (PLTP) to prevent bacterial growth and to treat sepsis |
| title_full |
Recombinant human plasma phospholipid transfer protein (PLTP) to prevent bacterial growth and to treat sepsis |
| title_fullStr |
Recombinant human plasma phospholipid transfer protein (PLTP) to prevent bacterial growth and to treat sepsis |
| title_full_unstemmed |
Recombinant human plasma phospholipid transfer protein (PLTP) to prevent bacterial growth and to treat sepsis |
| title_sort |
recombinant human plasma phospholipid transfer protein (pltp) to prevent bacterial growth and to treat sepsis |
| publisher |
Nature Portfolio |
| publishDate |
2017 |
| url |
https://doaj.org/article/38bc086aee0d4b64bfb9adb2801c05a8 |
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