Diagnosis of Carrion's disease by direct blood PCR in thin blood smear negative samples.

Bartonella bacilliformis is the etiologic agent of Carrion's disease. This disease has two well established phases, the most relevant being the so called Oroya Fever, in which B. bacilliformis infect the erythrocytes resulting in severe anemia and transient immunosuppression, with a high lethal...

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Auteurs principaux:	Juana del Valle Mendoza, Wilmer Silva Caso, Carmen Tinco Valdez, Maria J Pons, Luis J del Valle, Verónica Casabona Oré, Denisse Champin Michelena, Jorge Bazán Mayra, Víctor Zavaleta Gavidea, Martha Vargas, Joaquim Ruiz
Format:	article
Langue:	EN
Publié:	Public Library of Science (PLoS) 2014
Sujets:	Medicine R Science Q
Accès en ligne:	https://doaj.org/article/38bf8b97279544f0a1e05a5e89208e2d
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spelling	oai:doaj.org-article:38bf8b97279544f0a1e05a5e89208e2d2021-11-18T08:27:08ZDiagnosis of Carrion's disease by direct blood PCR in thin blood smear negative samples.1932-620310.1371/journal.pone.0092283https://doaj.org/article/38bf8b97279544f0a1e05a5e89208e2d2014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24651298/?tool=EBIhttps://doaj.org/toc/1932-6203Bartonella bacilliformis is the etiologic agent of Carrion's disease. This disease has two well established phases, the most relevant being the so called Oroya Fever, in which B. bacilliformis infect the erythrocytes resulting in severe anemia and transient immunosuppression, with a high lethality in the absence of adequate antibiotic treatment. The presence of B. bacilliformis was studied in 113 blood samples suspected of Carrion's disease based on clinical criteria, despite the absence of a positive thin blood smear, by two different PCR techniques (using Bartonella-specific and universal 16S rRNA gene primers), and by bacterial culture. The specific 16S rRNA gene primers revealed the presence of 21 B. bacilliformis and 1 Bartonella elizabethae, while universal primers showed both the presence of 3 coinfections in which a concomitant pathogen was detected plus Bartonella, in addition to the presence of infections by other microorganisms such as Agrobacterium or Bacillus firmus. These data support the need to implement molecular tools to diagnose Carrion's disease.Juana del Valle MendozaWilmer Silva CasoCarmen Tinco ValdezMaria J PonsLuis J del ValleVerónica Casabona OréDenisse Champin MichelenaJorge Bazán MayraVíctor Zavaleta GavideaMartha VargasJoaquim RuizPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 3, p e92283 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Juana del Valle Mendoza Wilmer Silva Caso Carmen Tinco Valdez Maria J Pons Luis J del Valle Verónica Casabona Oré Denisse Champin Michelena Jorge Bazán Mayra Víctor Zavaleta Gavidea Martha Vargas Joaquim Ruiz Diagnosis of Carrion's disease by direct blood PCR in thin blood smear negative samples.
description	Bartonella bacilliformis is the etiologic agent of Carrion's disease. This disease has two well established phases, the most relevant being the so called Oroya Fever, in which B. bacilliformis infect the erythrocytes resulting in severe anemia and transient immunosuppression, with a high lethality in the absence of adequate antibiotic treatment. The presence of B. bacilliformis was studied in 113 blood samples suspected of Carrion's disease based on clinical criteria, despite the absence of a positive thin blood smear, by two different PCR techniques (using Bartonella-specific and universal 16S rRNA gene primers), and by bacterial culture. The specific 16S rRNA gene primers revealed the presence of 21 B. bacilliformis and 1 Bartonella elizabethae, while universal primers showed both the presence of 3 coinfections in which a concomitant pathogen was detected plus Bartonella, in addition to the presence of infections by other microorganisms such as Agrobacterium or Bacillus firmus. These data support the need to implement molecular tools to diagnose Carrion's disease.
format	article
author	Juana del Valle Mendoza Wilmer Silva Caso Carmen Tinco Valdez Maria J Pons Luis J del Valle Verónica Casabona Oré Denisse Champin Michelena Jorge Bazán Mayra Víctor Zavaleta Gavidea Martha Vargas Joaquim Ruiz
author_facet	Juana del Valle Mendoza Wilmer Silva Caso Carmen Tinco Valdez Maria J Pons Luis J del Valle Verónica Casabona Oré Denisse Champin Michelena Jorge Bazán Mayra Víctor Zavaleta Gavidea Martha Vargas Joaquim Ruiz
author_sort	Juana del Valle Mendoza
title	Diagnosis of Carrion's disease by direct blood PCR in thin blood smear negative samples.
title_short	Diagnosis of Carrion's disease by direct blood PCR in thin blood smear negative samples.
title_full	Diagnosis of Carrion's disease by direct blood PCR in thin blood smear negative samples.
title_fullStr	Diagnosis of Carrion's disease by direct blood PCR in thin blood smear negative samples.
title_full_unstemmed	Diagnosis of Carrion's disease by direct blood PCR in thin blood smear negative samples.
title_sort	diagnosis of carrion's disease by direct blood pcr in thin blood smear negative samples.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/38bf8b97279544f0a1e05a5e89208e2d
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Diagnosis of Carrion's disease by direct blood PCR in thin blood smear negative samples.

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