Identification of a genetic variant underlying familial cases of recurrent benign paroxysmal positional vertigo.

Benign paroxysmal positional vertigo (BPPV) is the most common cause of vertigo in humans, yet the molecular etiology is currently unknown. Evidence suggests that genetic factors may play an important role in some cases of idiopathic BPPV, particularly in familial cases, but the responsible genetic...

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Autores principales: Yinfang Xu, Yan Zhang, Ivan A Lopez, Jacey Hilbers, Anthony J Griswold, Akira Ishiyama, Susan Blanton, Xue Zhong Liu, Yunxia Wang Lundberg
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spelling oai:doaj.org-article:38c2bbb8d12249e78cb682cf7cdeecd52021-11-25T06:19:17ZIdentification of a genetic variant underlying familial cases of recurrent benign paroxysmal positional vertigo.1932-620310.1371/journal.pone.0251386https://doaj.org/article/38c2bbb8d12249e78cb682cf7cdeecd52021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0251386https://doaj.org/toc/1932-6203Benign paroxysmal positional vertigo (BPPV) is the most common cause of vertigo in humans, yet the molecular etiology is currently unknown. Evidence suggests that genetic factors may play an important role in some cases of idiopathic BPPV, particularly in familial cases, but the responsible genetic variants have not been identified. In this study, we performed whole exome sequencing [including untranslated regions (UTRs)] of 12 families and Sanger sequencing of additional 30 families with recurrent BPPV in Caucasians from the United States (US) Midwest region, to identify the genetic variants responsible for heightened susceptibility to BPPV. Fifty non-BPPV families were included as controls. In silico and experimental analyses of candidate variants show that an insertion variant rs113784532 (frameshift causing truncation) in the neural cadherin gene PCDHGA10 (protocadherin-gamma A10) is an exceedingly strong candidate (p = 1.80x10-4 vs. sample controls; p = 5.85x10-19 vs. ExAC data; p = 4.9x10-3 vs. NHLBI exome data). The mutant protein forms large aggregates in BPPV samples even at young ages, and affected subjects carrying this variant have an earlier onset of the condition than those without [average 44.0±14.0 (n = 16) versus 54.4±16.1 (n = 36) years old, p = 0.054]. In both human and mouse inner ear tissues, PCDHGA10 is expressed in ganglia, hair cells and vestibular transitional epithelia. Fluorescent RNA in situ hybridization using mouse inner ear tissues shows that expression increases with age. In summary, our data show that a variant in the PCDHGA10 gene may be involved in causing or aggravating some familial cases of recurrent idiopathic BPPV.Yinfang XuYan ZhangIvan A LopezJacey HilbersAnthony J GriswoldAkira IshiyamaSusan BlantonXue Zhong LiuYunxia Wang LundbergPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 5, p e0251386 (2021)
institution DOAJ
collection DOAJ
language EN
topic Medicine
R
Science
Q
spellingShingle Medicine
R
Science
Q
Yinfang Xu
Yan Zhang
Ivan A Lopez
Jacey Hilbers
Anthony J Griswold
Akira Ishiyama
Susan Blanton
Xue Zhong Liu
Yunxia Wang Lundberg
Identification of a genetic variant underlying familial cases of recurrent benign paroxysmal positional vertigo.
description Benign paroxysmal positional vertigo (BPPV) is the most common cause of vertigo in humans, yet the molecular etiology is currently unknown. Evidence suggests that genetic factors may play an important role in some cases of idiopathic BPPV, particularly in familial cases, but the responsible genetic variants have not been identified. In this study, we performed whole exome sequencing [including untranslated regions (UTRs)] of 12 families and Sanger sequencing of additional 30 families with recurrent BPPV in Caucasians from the United States (US) Midwest region, to identify the genetic variants responsible for heightened susceptibility to BPPV. Fifty non-BPPV families were included as controls. In silico and experimental analyses of candidate variants show that an insertion variant rs113784532 (frameshift causing truncation) in the neural cadherin gene PCDHGA10 (protocadherin-gamma A10) is an exceedingly strong candidate (p = 1.80x10-4 vs. sample controls; p = 5.85x10-19 vs. ExAC data; p = 4.9x10-3 vs. NHLBI exome data). The mutant protein forms large aggregates in BPPV samples even at young ages, and affected subjects carrying this variant have an earlier onset of the condition than those without [average 44.0±14.0 (n = 16) versus 54.4±16.1 (n = 36) years old, p = 0.054]. In both human and mouse inner ear tissues, PCDHGA10 is expressed in ganglia, hair cells and vestibular transitional epithelia. Fluorescent RNA in situ hybridization using mouse inner ear tissues shows that expression increases with age. In summary, our data show that a variant in the PCDHGA10 gene may be involved in causing or aggravating some familial cases of recurrent idiopathic BPPV.
format article
author Yinfang Xu
Yan Zhang
Ivan A Lopez
Jacey Hilbers
Anthony J Griswold
Akira Ishiyama
Susan Blanton
Xue Zhong Liu
Yunxia Wang Lundberg
author_facet Yinfang Xu
Yan Zhang
Ivan A Lopez
Jacey Hilbers
Anthony J Griswold
Akira Ishiyama
Susan Blanton
Xue Zhong Liu
Yunxia Wang Lundberg
author_sort Yinfang Xu
title Identification of a genetic variant underlying familial cases of recurrent benign paroxysmal positional vertigo.
title_short Identification of a genetic variant underlying familial cases of recurrent benign paroxysmal positional vertigo.
title_full Identification of a genetic variant underlying familial cases of recurrent benign paroxysmal positional vertigo.
title_fullStr Identification of a genetic variant underlying familial cases of recurrent benign paroxysmal positional vertigo.
title_full_unstemmed Identification of a genetic variant underlying familial cases of recurrent benign paroxysmal positional vertigo.
title_sort identification of a genetic variant underlying familial cases of recurrent benign paroxysmal positional vertigo.
publisher Public Library of Science (PLoS)
publishDate 2021
url https://doaj.org/article/38c2bbb8d12249e78cb682cf7cdeecd5
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