Directing the Self-assembly of Tumour Spheroids by Bioprinting Cellular Heterogeneous Models within Alginate/Gelatin Hydrogels

Abstract Human tumour progression is a dynamic process involving diverse biological and biochemical events such as genetic mutation and selection in addition to physical, chemical, and mechanical events occurring between cells and the tumour microenvironment. Using 3D bioprinting we have developed a...

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Autores principales:	Tao Jiang, Jose G. Munguia-Lopez, Salvador Flores-Torres, Joel Grant, Sanahan Vijayakumar, Antonio De Leon-Rodriguez, Joseph M. Kinsella
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/38c59d3912fc4e5f8b064a0d87e742e5
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spelling	oai:doaj.org-article:38c59d3912fc4e5f8b064a0d87e742e52021-12-02T16:08:07ZDirecting the Self-assembly of Tumour Spheroids by Bioprinting Cellular Heterogeneous Models within Alginate/Gelatin Hydrogels10.1038/s41598-017-04691-92045-2322https://doaj.org/article/38c59d3912fc4e5f8b064a0d87e742e52017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-04691-9https://doaj.org/toc/2045-2322Abstract Human tumour progression is a dynamic process involving diverse biological and biochemical events such as genetic mutation and selection in addition to physical, chemical, and mechanical events occurring between cells and the tumour microenvironment. Using 3D bioprinting we have developed a method to embed MDA-MB-231 triple negative breast cancer cells, and IMR-90 fibroblast cells, within a cross-linked alginate/gelatin matrix at specific initial locations relative to each other. After 7 days of co-culture the MDA-MB-231 cells begin to form multicellular tumour spheroids (MCTS) that increase in size and frequency over time. After ~15 days the IMR-90 stromal fibroblast cells migrate through a non-cellularized region of the hydrogel matrix and infiltrate the MDA-MB-231 spheroids creating mixed MDA-MB-231/IMR-90 MCTS. This study provides a proof-of-concept that biomimetic in vitro tissue co-culture models bioprinted with both breast cancer cells and fibroblasts will result in MCTS that can be maintained for durations of several weeks.Tao JiangJose G. Munguia-LopezSalvador Flores-TorresJoel GrantSanahan VijayakumarAntonio De Leon-RodriguezJoseph M. KinsellaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-9 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Tao Jiang Jose G. Munguia-Lopez Salvador Flores-Torres Joel Grant Sanahan Vijayakumar Antonio De Leon-Rodriguez Joseph M. Kinsella Directing the Self-assembly of Tumour Spheroids by Bioprinting Cellular Heterogeneous Models within Alginate/Gelatin Hydrogels
description	Abstract Human tumour progression is a dynamic process involving diverse biological and biochemical events such as genetic mutation and selection in addition to physical, chemical, and mechanical events occurring between cells and the tumour microenvironment. Using 3D bioprinting we have developed a method to embed MDA-MB-231 triple negative breast cancer cells, and IMR-90 fibroblast cells, within a cross-linked alginate/gelatin matrix at specific initial locations relative to each other. After 7 days of co-culture the MDA-MB-231 cells begin to form multicellular tumour spheroids (MCTS) that increase in size and frequency over time. After ~15 days the IMR-90 stromal fibroblast cells migrate through a non-cellularized region of the hydrogel matrix and infiltrate the MDA-MB-231 spheroids creating mixed MDA-MB-231/IMR-90 MCTS. This study provides a proof-of-concept that biomimetic in vitro tissue co-culture models bioprinted with both breast cancer cells and fibroblasts will result in MCTS that can be maintained for durations of several weeks.
format	article
author	Tao Jiang Jose G. Munguia-Lopez Salvador Flores-Torres Joel Grant Sanahan Vijayakumar Antonio De Leon-Rodriguez Joseph M. Kinsella
author_facet	Tao Jiang Jose G. Munguia-Lopez Salvador Flores-Torres Joel Grant Sanahan Vijayakumar Antonio De Leon-Rodriguez Joseph M. Kinsella
author_sort	Tao Jiang
title	Directing the Self-assembly of Tumour Spheroids by Bioprinting Cellular Heterogeneous Models within Alginate/Gelatin Hydrogels
title_short	Directing the Self-assembly of Tumour Spheroids by Bioprinting Cellular Heterogeneous Models within Alginate/Gelatin Hydrogels
title_full	Directing the Self-assembly of Tumour Spheroids by Bioprinting Cellular Heterogeneous Models within Alginate/Gelatin Hydrogels
title_fullStr	Directing the Self-assembly of Tumour Spheroids by Bioprinting Cellular Heterogeneous Models within Alginate/Gelatin Hydrogels
title_full_unstemmed	Directing the Self-assembly of Tumour Spheroids by Bioprinting Cellular Heterogeneous Models within Alginate/Gelatin Hydrogels
title_sort	directing the self-assembly of tumour spheroids by bioprinting cellular heterogeneous models within alginate/gelatin hydrogels
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/38c59d3912fc4e5f8b064a0d87e742e5
work_keys_str_mv	AT taojiang directingtheselfassemblyoftumourspheroidsbybioprintingcellularheterogeneousmodelswithinalginategelatinhydrogels AT josegmunguialopez directingtheselfassemblyoftumourspheroidsbybioprintingcellularheterogeneousmodelswithinalginategelatinhydrogels AT salvadorflorestorres directingtheselfassemblyoftumourspheroidsbybioprintingcellularheterogeneousmodelswithinalginategelatinhydrogels AT joelgrant directingtheselfassemblyoftumourspheroidsbybioprintingcellularheterogeneousmodelswithinalginategelatinhydrogels AT sanahanvijayakumar directingtheselfassemblyoftumourspheroidsbybioprintingcellularheterogeneousmodelswithinalginategelatinhydrogels AT antoniodeleonrodriguez directingtheselfassemblyoftumourspheroidsbybioprintingcellularheterogeneousmodelswithinalginategelatinhydrogels AT josephmkinsella directingtheselfassemblyoftumourspheroidsbybioprintingcellularheterogeneousmodelswithinalginategelatinhydrogels
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Directing the Self-assembly of Tumour Spheroids by Bioprinting Cellular Heterogeneous Models within Alginate/Gelatin Hydrogels

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