Antimicrobial Effects of <i>Inula viscosa</i> Extract on the In Situ Initial Oral Biofilm

Given the undesirable side effects of commercially used mouth rinses that include chemically synthesized antimicrobial compounds such as chlorhexidine, it is essential to discover novel antimicrobial substances based on plant extracts. The aim of this study was to examine the antimicrobial effect of...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Hannah Kurz, Lamprini Karygianni, Aikaterini Argyropoulou, Elmar Hellwig, Alexios Leandros Skaltsounis, Annette Wittmer, Kirstin Vach, Ali Al-Ahmad
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	<i>Inula viscosa</i> initial adhesion colony-forming units (CFU) live/dead staining fluorescence microscopy Nutrition. Foods and food supply TX341-641
Acceso en línea:	https://doaj.org/article/38d35ea0c2b9496a920a44b9d927e076
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:38d35ea0c2b9496a920a44b9d927e076
record_format	dspace
spelling	oai:doaj.org-article:38d35ea0c2b9496a920a44b9d927e0762021-11-25T18:36:14ZAntimicrobial Effects of <i>Inula viscosa</i> Extract on the In Situ Initial Oral Biofilm10.3390/nu131140292072-6643https://doaj.org/article/38d35ea0c2b9496a920a44b9d927e0762021-11-01T00:00:00Zhttps://www.mdpi.com/2072-6643/13/11/4029https://doaj.org/toc/2072-6643Given the undesirable side effects of commercially used mouth rinses that include chemically synthesized antimicrobial compounds such as chlorhexidine, it is essential to discover novel antimicrobial substances based on plant extracts. The aim of this study was to examine the antimicrobial effect of <i>Inula viscosa</i> extract on the initial microbial adhesion in the oral cavity. Individual test splints were manufactured for the participants, on which disinfected bovine enamel samples were attached. After the initial microbial adhesion, the biofilm-covered oral samples were removed and treated with different concentrations (10, 20, and 30 mg/mL) of an <i>I. viscosa</i> extract for 10 min. Positive and negative controls were also sampled. Regarding the microbiological parameters, the colony-forming units (CFU) and vitality testing (live/dead staining) were examined in combination with fluorescence microscopy. An <i>I. viscosa</i> extract with a concentration of 30 mg/mL killed the bacteria of the initial adhesion at a rate of 99.99% (log<sub>10</sub> CFU value of 1.837 ± 1.54). Compared to the negative control, no killing effects were determined after treatment with <i>I. viscosa</i> extract at concentrations of 10 mg/mL (log<sub>10</sub> CFU value 3.776 ± 0.831; median 3.776) and 20 mg/mL (log<sub>10</sub> CFU value 3.725 ± 0.300; median 3.711). The live/dead staining revealed a significant reduction (<i>p</i> < 0.0001) of vital adherent bacteria after treatment with 10 mg/mL of <i>I. viscosa</i> extract. After treatment with an <i>I. viscosa</i> extract with a concentration of 30 mg/mL, no vital bacteria could be detected. For the first time, significant antimicrobial effects on the initial microbial adhesion in in situ oral biofilms were reported for an <i>I. viscosa</i> extract.Hannah KurzLamprini KarygianniAikaterini ArgyropoulouElmar HellwigAlexios Leandros SkaltsounisAnnette WittmerKirstin VachAli Al-AhmadMDPI AGarticle<i>Inula viscosa</i>initial adhesioncolony-forming units (CFU)live/dead stainingfluorescence microscopyNutrition. Foods and food supplyTX341-641ENNutrients, Vol 13, Iss 4029, p 4029 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	<i>Inula viscosa</i> initial adhesion colony-forming units (CFU) live/dead staining fluorescence microscopy Nutrition. Foods and food supply TX341-641
spellingShingle	<i>Inula viscosa</i> initial adhesion colony-forming units (CFU) live/dead staining fluorescence microscopy Nutrition. Foods and food supply TX341-641 Hannah Kurz Lamprini Karygianni Aikaterini Argyropoulou Elmar Hellwig Alexios Leandros Skaltsounis Annette Wittmer Kirstin Vach Ali Al-Ahmad Antimicrobial Effects of <i>Inula viscosa</i> Extract on the In Situ Initial Oral Biofilm
description	Given the undesirable side effects of commercially used mouth rinses that include chemically synthesized antimicrobial compounds such as chlorhexidine, it is essential to discover novel antimicrobial substances based on plant extracts. The aim of this study was to examine the antimicrobial effect of <i>Inula viscosa</i> extract on the initial microbial adhesion in the oral cavity. Individual test splints were manufactured for the participants, on which disinfected bovine enamel samples were attached. After the initial microbial adhesion, the biofilm-covered oral samples were removed and treated with different concentrations (10, 20, and 30 mg/mL) of an <i>I. viscosa</i> extract for 10 min. Positive and negative controls were also sampled. Regarding the microbiological parameters, the colony-forming units (CFU) and vitality testing (live/dead staining) were examined in combination with fluorescence microscopy. An <i>I. viscosa</i> extract with a concentration of 30 mg/mL killed the bacteria of the initial adhesion at a rate of 99.99% (log<sub>10</sub> CFU value of 1.837 ± 1.54). Compared to the negative control, no killing effects were determined after treatment with <i>I. viscosa</i> extract at concentrations of 10 mg/mL (log<sub>10</sub> CFU value 3.776 ± 0.831; median 3.776) and 20 mg/mL (log<sub>10</sub> CFU value 3.725 ± 0.300; median 3.711). The live/dead staining revealed a significant reduction (<i>p</i> < 0.0001) of vital adherent bacteria after treatment with 10 mg/mL of <i>I. viscosa</i> extract. After treatment with an <i>I. viscosa</i> extract with a concentration of 30 mg/mL, no vital bacteria could be detected. For the first time, significant antimicrobial effects on the initial microbial adhesion in in situ oral biofilms were reported for an <i>I. viscosa</i> extract.
format	article
author	Hannah Kurz Lamprini Karygianni Aikaterini Argyropoulou Elmar Hellwig Alexios Leandros Skaltsounis Annette Wittmer Kirstin Vach Ali Al-Ahmad
author_facet	Hannah Kurz Lamprini Karygianni Aikaterini Argyropoulou Elmar Hellwig Alexios Leandros Skaltsounis Annette Wittmer Kirstin Vach Ali Al-Ahmad
author_sort	Hannah Kurz
title	Antimicrobial Effects of <i>Inula viscosa</i> Extract on the In Situ Initial Oral Biofilm
title_short	Antimicrobial Effects of <i>Inula viscosa</i> Extract on the In Situ Initial Oral Biofilm
title_full	Antimicrobial Effects of <i>Inula viscosa</i> Extract on the In Situ Initial Oral Biofilm
title_fullStr	Antimicrobial Effects of <i>Inula viscosa</i> Extract on the In Situ Initial Oral Biofilm
title_full_unstemmed	Antimicrobial Effects of <i>Inula viscosa</i> Extract on the In Situ Initial Oral Biofilm
title_sort	antimicrobial effects of <i>inula viscosa</i> extract on the in situ initial oral biofilm
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/38d35ea0c2b9496a920a44b9d927e076
work_keys_str_mv	AT hannahkurz antimicrobialeffectsofiinulaviscosaiextractontheinsituinitialoralbiofilm AT lamprinikarygianni antimicrobialeffectsofiinulaviscosaiextractontheinsituinitialoralbiofilm AT aikateriniargyropoulou antimicrobialeffectsofiinulaviscosaiextractontheinsituinitialoralbiofilm AT elmarhellwig antimicrobialeffectsofiinulaviscosaiextractontheinsituinitialoralbiofilm AT alexiosleandrosskaltsounis antimicrobialeffectsofiinulaviscosaiextractontheinsituinitialoralbiofilm AT annettewittmer antimicrobialeffectsofiinulaviscosaiextractontheinsituinitialoralbiofilm AT kirstinvach antimicrobialeffectsofiinulaviscosaiextractontheinsituinitialoralbiofilm AT alialahmad antimicrobialeffectsofiinulaviscosaiextractontheinsituinitialoralbiofilm
_version_	1718410886115229696

Antimicrobial Effects of <i>Inula viscosa</i> Extract on the In Situ Initial Oral Biofilm

Ejemplares similares