Photodynamic antimicrobial chemotherapy in aquaculture: photoinactivation studies of Vibrio fischeri.

<h4>Background</h4>Photodynamic antimicrobial chemotherapy (PACT) combines light, a light-absorbing molecule that initiates a photochemical or photophysical reaction, and oxygen. The combined action of these three components originates reactive oxygen species that lead to microorganisms&...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Eliana Alves, Maria A F Faustino, João P C Tomé, Maria G P M S Neves, Augusto C Tomé, José A S Cavaleiro, Ângela Cunha, Newton C M Gomes, Adelaide Almeida
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2011
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/38d75252bf5c445293eff9e103d3375e
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:38d75252bf5c445293eff9e103d3375e
record_format	dspace
spelling	oai:doaj.org-article:38d75252bf5c445293eff9e103d3375e2021-11-18T06:51:47ZPhotodynamic antimicrobial chemotherapy in aquaculture: photoinactivation studies of Vibrio fischeri.1932-620310.1371/journal.pone.0020970https://doaj.org/article/38d75252bf5c445293eff9e103d3375e2011-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21698119/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>Photodynamic antimicrobial chemotherapy (PACT) combines light, a light-absorbing molecule that initiates a photochemical or photophysical reaction, and oxygen. The combined action of these three components originates reactive oxygen species that lead to microorganisms' destruction. The aim was to evaluate the efficiency of PACT on Vibrio fischeri: 1) with buffer solution, varying temperature, pH, salinity and oxygen concentration values; 2) with aquaculture water, to reproduce photoinactivation (PI) conditions in situ.<h4>Methodology/principal findings</h4>To monitor the PI kinetics, the bioluminescence of V. fischeri was measured during the experiments. A tricationic meso-substituted porphyrin (Tri-Py(+)-Me-PF) was used as photosensitizer (5 µM in the studies with buffer solution and 10-50 µM in the studies with aquaculture water); artificial white light (4 mW cm(-2)) and solar irradiation (40 mW cm(-2)) were used as light sources; and the bacterial concentration used for all experiments was ≈10(7) CFU mL(-1) (corresponding to a bioluminescence level of 10(5) relative light units--RLU). The variations in pH (6.5-8.5), temperature (10-25°C), salinity (20-40 g L(-1)) and oxygen concentration did not significantly affect the PI of V. fischeri, once in all tested conditions the bioluminescent signal decreased to the detection limit of the method (≈7 log reduction). The assays using aquaculture water showed that the efficiency of the process is affected by the suspended matter. Total PI of V. fischeri in aquaculture water was achieved under solar light in the presence of 20 µM of Tri-Py(+)-Me-PF.<h4>Conclusions/significance</h4>If PACT is to be used in environmental applications, the matrix containing target microbial communities should be previously characterized in order to establish an efficient protocol having into account the photosensitizer concentration, the light source and the total light dose delivered. The possibility of using solar light in PACT to treat aquaculture water makes this technology cost-effective and attractive.Eliana AlvesMaria A F FaustinoJoão P C ToméMaria G P M S NevesAugusto C ToméJosé A S CavaleiroÂngela CunhaNewton C M GomesAdelaide AlmeidaPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 6, Iss 6, p e20970 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Eliana Alves Maria A F Faustino João P C Tomé Maria G P M S Neves Augusto C Tomé José A S Cavaleiro Ângela Cunha Newton C M Gomes Adelaide Almeida Photodynamic antimicrobial chemotherapy in aquaculture: photoinactivation studies of Vibrio fischeri.
description	<h4>Background</h4>Photodynamic antimicrobial chemotherapy (PACT) combines light, a light-absorbing molecule that initiates a photochemical or photophysical reaction, and oxygen. The combined action of these three components originates reactive oxygen species that lead to microorganisms' destruction. The aim was to evaluate the efficiency of PACT on Vibrio fischeri: 1) with buffer solution, varying temperature, pH, salinity and oxygen concentration values; 2) with aquaculture water, to reproduce photoinactivation (PI) conditions in situ.<h4>Methodology/principal findings</h4>To monitor the PI kinetics, the bioluminescence of V. fischeri was measured during the experiments. A tricationic meso-substituted porphyrin (Tri-Py(+)-Me-PF) was used as photosensitizer (5 µM in the studies with buffer solution and 10-50 µM in the studies with aquaculture water); artificial white light (4 mW cm(-2)) and solar irradiation (40 mW cm(-2)) were used as light sources; and the bacterial concentration used for all experiments was ≈10(7) CFU mL(-1) (corresponding to a bioluminescence level of 10(5) relative light units--RLU). The variations in pH (6.5-8.5), temperature (10-25°C), salinity (20-40 g L(-1)) and oxygen concentration did not significantly affect the PI of V. fischeri, once in all tested conditions the bioluminescent signal decreased to the detection limit of the method (≈7 log reduction). The assays using aquaculture water showed that the efficiency of the process is affected by the suspended matter. Total PI of V. fischeri in aquaculture water was achieved under solar light in the presence of 20 µM of Tri-Py(+)-Me-PF.<h4>Conclusions/significance</h4>If PACT is to be used in environmental applications, the matrix containing target microbial communities should be previously characterized in order to establish an efficient protocol having into account the photosensitizer concentration, the light source and the total light dose delivered. The possibility of using solar light in PACT to treat aquaculture water makes this technology cost-effective and attractive.
format	article
author	Eliana Alves Maria A F Faustino João P C Tomé Maria G P M S Neves Augusto C Tomé José A S Cavaleiro Ângela Cunha Newton C M Gomes Adelaide Almeida
author_facet	Eliana Alves Maria A F Faustino João P C Tomé Maria G P M S Neves Augusto C Tomé José A S Cavaleiro Ângela Cunha Newton C M Gomes Adelaide Almeida
author_sort	Eliana Alves
title	Photodynamic antimicrobial chemotherapy in aquaculture: photoinactivation studies of Vibrio fischeri.
title_short	Photodynamic antimicrobial chemotherapy in aquaculture: photoinactivation studies of Vibrio fischeri.
title_full	Photodynamic antimicrobial chemotherapy in aquaculture: photoinactivation studies of Vibrio fischeri.
title_fullStr	Photodynamic antimicrobial chemotherapy in aquaculture: photoinactivation studies of Vibrio fischeri.
title_full_unstemmed	Photodynamic antimicrobial chemotherapy in aquaculture: photoinactivation studies of Vibrio fischeri.
title_sort	photodynamic antimicrobial chemotherapy in aquaculture: photoinactivation studies of vibrio fischeri.
publisher	Public Library of Science (PLoS)
publishDate	2011
url	https://doaj.org/article/38d75252bf5c445293eff9e103d3375e
work_keys_str_mv	AT elianaalves photodynamicantimicrobialchemotherapyinaquaculturephotoinactivationstudiesofvibriofischeri AT mariaaffaustino photodynamicantimicrobialchemotherapyinaquaculturephotoinactivationstudiesofvibriofischeri AT joaopctome photodynamicantimicrobialchemotherapyinaquaculturephotoinactivationstudiesofvibriofischeri AT mariagpmsneves photodynamicantimicrobialchemotherapyinaquaculturephotoinactivationstudiesofvibriofischeri AT augustoctome photodynamicantimicrobialchemotherapyinaquaculturephotoinactivationstudiesofvibriofischeri AT joseascavaleiro photodynamicantimicrobialchemotherapyinaquaculturephotoinactivationstudiesofvibriofischeri AT angelacunha photodynamicantimicrobialchemotherapyinaquaculturephotoinactivationstudiesofvibriofischeri AT newtoncmgomes photodynamicantimicrobialchemotherapyinaquaculturephotoinactivationstudiesofvibriofischeri AT adelaidealmeida photodynamicantimicrobialchemotherapyinaquaculturephotoinactivationstudiesofvibriofischeri
_version_	1718424284747005952

Photodynamic antimicrobial chemotherapy in aquaculture: photoinactivation studies of Vibrio fischeri.

Ejemplares similares