Transcription factor NF-κB is modulated by symbiotic status in a sea anemone model of cnidarian bleaching

Abstract Transcription factor NF-κB plays a central role in immunity from fruit flies to humans, and NF-κB activity is altered in many human diseases. To investigate a role for NF-κB in immunity and disease on a broader evolutionary scale we have characterized NF-κB in a sea anemone (Exaiptasia pall...

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Autores principales:	Katelyn M. Mansfield, Nicole M. Carter, Linda Nguyen, Phillip A. Cleves, Anar Alshanbayeva, Leah M. Williams, Camerron Crowder, Ashley R. Penvose, John R. Finnerty, Virginia M. Weis, Trevor W. Siggers, Thomas D. Gilmore
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/38db6b54c01449c3813782268876d0d4
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spelling	oai:doaj.org-article:38db6b54c01449c3813782268876d0d42021-12-02T15:05:04ZTranscription factor NF-κB is modulated by symbiotic status in a sea anemone model of cnidarian bleaching10.1038/s41598-017-16168-w2045-2322https://doaj.org/article/38db6b54c01449c3813782268876d0d42017-11-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-16168-whttps://doaj.org/toc/2045-2322Abstract Transcription factor NF-κB plays a central role in immunity from fruit flies to humans, and NF-κB activity is altered in many human diseases. To investigate a role for NF-κB in immunity and disease on a broader evolutionary scale we have characterized NF-κB in a sea anemone (Exaiptasia pallida; called Aiptasia herein) model for cnidarian symbiosis and dysbiosis (i.e., “bleaching”). We show that the DNA-binding site specificity of Aiptasia NF-κB is similar to NF-κB proteins from a broad expanse of organisms. Analyses of NF-κB and IκB kinase proteins from Aiptasia suggest that non-canonical NF-κB processing is an evolutionarily ancient pathway, which can be reconstituted in human cells. In Aiptasia, NF-κB protein levels, DNA-binding activity, and tissue expression increase when loss of the algal symbiont Symbiodinium is induced by heat or chemical treatment. Kinetic analysis of NF-κB levels following loss of symbiosis show that NF-κB levels increase only after Symbiodinium is cleared. Moreover, introduction of Symbiodinium into naïve Aiptasia larvae results in a decrease in NF-κB expression. Our results suggest that Symbiodinium suppresses NF-κB in order to enable establishment of symbiosis in Aiptasia. These results are the first to demonstrate a link between changes in the conserved immune regulatory protein NF-κB and cnidarian symbiotic status.Katelyn M. MansfieldNicole M. CarterLinda NguyenPhillip A. ClevesAnar AlshanbayevaLeah M. WilliamsCamerron CrowderAshley R. PenvoseJohn R. FinnertyVirginia M. WeisTrevor W. SiggersThomas D. GilmoreNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-14 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Katelyn M. Mansfield Nicole M. Carter Linda Nguyen Phillip A. Cleves Anar Alshanbayeva Leah M. Williams Camerron Crowder Ashley R. Penvose John R. Finnerty Virginia M. Weis Trevor W. Siggers Thomas D. Gilmore Transcription factor NF-κB is modulated by symbiotic status in a sea anemone model of cnidarian bleaching
description	Abstract Transcription factor NF-κB plays a central role in immunity from fruit flies to humans, and NF-κB activity is altered in many human diseases. To investigate a role for NF-κB in immunity and disease on a broader evolutionary scale we have characterized NF-κB in a sea anemone (Exaiptasia pallida; called Aiptasia herein) model for cnidarian symbiosis and dysbiosis (i.e., “bleaching”). We show that the DNA-binding site specificity of Aiptasia NF-κB is similar to NF-κB proteins from a broad expanse of organisms. Analyses of NF-κB and IκB kinase proteins from Aiptasia suggest that non-canonical NF-κB processing is an evolutionarily ancient pathway, which can be reconstituted in human cells. In Aiptasia, NF-κB protein levels, DNA-binding activity, and tissue expression increase when loss of the algal symbiont Symbiodinium is induced by heat or chemical treatment. Kinetic analysis of NF-κB levels following loss of symbiosis show that NF-κB levels increase only after Symbiodinium is cleared. Moreover, introduction of Symbiodinium into naïve Aiptasia larvae results in a decrease in NF-κB expression. Our results suggest that Symbiodinium suppresses NF-κB in order to enable establishment of symbiosis in Aiptasia. These results are the first to demonstrate a link between changes in the conserved immune regulatory protein NF-κB and cnidarian symbiotic status.
format	article
author	Katelyn M. Mansfield Nicole M. Carter Linda Nguyen Phillip A. Cleves Anar Alshanbayeva Leah M. Williams Camerron Crowder Ashley R. Penvose John R. Finnerty Virginia M. Weis Trevor W. Siggers Thomas D. Gilmore
author_facet	Katelyn M. Mansfield Nicole M. Carter Linda Nguyen Phillip A. Cleves Anar Alshanbayeva Leah M. Williams Camerron Crowder Ashley R. Penvose John R. Finnerty Virginia M. Weis Trevor W. Siggers Thomas D. Gilmore
author_sort	Katelyn M. Mansfield
title	Transcription factor NF-κB is modulated by symbiotic status in a sea anemone model of cnidarian bleaching
title_short	Transcription factor NF-κB is modulated by symbiotic status in a sea anemone model of cnidarian bleaching
title_full	Transcription factor NF-κB is modulated by symbiotic status in a sea anemone model of cnidarian bleaching
title_fullStr	Transcription factor NF-κB is modulated by symbiotic status in a sea anemone model of cnidarian bleaching
title_full_unstemmed	Transcription factor NF-κB is modulated by symbiotic status in a sea anemone model of cnidarian bleaching
title_sort	transcription factor nf-κb is modulated by symbiotic status in a sea anemone model of cnidarian bleaching
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/38db6b54c01449c3813782268876d0d4
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Transcription factor NF-κB is modulated by symbiotic status in a sea anemone model of cnidarian bleaching

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