Interdental Plaque Microbial Community Changes under In Vitro Violet LED Irradiation

Oral microbiome dysbiosis has important links to human health and disease. Although photodynamic therapy influences microbiome diversity, the specific effect of violet light irradiation remains largely unknown. In this study, we analyzed the effect of violet light-emitting diode (LED) irradiation on...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Dan Wang, Takayuki Nambu, Hiroaki Tanimoto, Naohiro Iwata, Kazushi Yoshikawa, Toshinori Okinaga, Kazuyo Yamamoto
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	oral microbiota violet light 16S rRNA gene high-throughput sequencing Therapeutics. Pharmacology RM1-950
Acceso en línea:	https://doaj.org/article/38e0bb091fcd4231b50a70c767fe8dc3
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:38e0bb091fcd4231b50a70c767fe8dc3
record_format	dspace
spelling	oai:doaj.org-article:38e0bb091fcd4231b50a70c767fe8dc32021-11-25T16:23:17ZInterdental Plaque Microbial Community Changes under In Vitro Violet LED Irradiation10.3390/antibiotics101113482079-6382https://doaj.org/article/38e0bb091fcd4231b50a70c767fe8dc32021-11-01T00:00:00Zhttps://www.mdpi.com/2079-6382/10/11/1348https://doaj.org/toc/2079-6382Oral microbiome dysbiosis has important links to human health and disease. Although photodynamic therapy influences microbiome diversity, the specific effect of violet light irradiation remains largely unknown. In this study, we analyzed the effect of violet light-emitting diode (LED) irradiation on interdental plaque microbiota. Interdental plaque was collected from 12 human subjects, exposed to violet LED irradiation, and cultured in a specialized growth medium. Next-generation sequencing of the 16S ribosomal RNA genes revealed that α-diversity decreased, whereas β-diversity exhibited a continuous change with violet LED irradiation doses. In addition, we identified several operational taxonomic units that exhibited significant shifts during violet LED irradiation. Specifically, violet LED irradiation led to a significant reduction in the relative abundance of <i>Fusobacterium</i> species, but a significant increase in several species of oral bacteria, such as <i>Veillonella</i> and <i>Campylobacter</i>. Our study provides an overview of oral plaque microbiota changes under violet LED irradiation, and highlights the potential of this method for adjusting the balance of the oral microbiome without inducing antibiotic resistance.Dan WangTakayuki NambuHiroaki TanimotoNaohiro IwataKazushi YoshikawaToshinori OkinagaKazuyo YamamotoMDPI AGarticleoral microbiotaviolet light16S rRNA genehigh-throughput sequencingTherapeutics. PharmacologyRM1-950ENAntibiotics, Vol 10, Iss 1348, p 1348 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	oral microbiota violet light 16S rRNA gene high-throughput sequencing Therapeutics. Pharmacology RM1-950
spellingShingle	oral microbiota violet light 16S rRNA gene high-throughput sequencing Therapeutics. Pharmacology RM1-950 Dan Wang Takayuki Nambu Hiroaki Tanimoto Naohiro Iwata Kazushi Yoshikawa Toshinori Okinaga Kazuyo Yamamoto Interdental Plaque Microbial Community Changes under In Vitro Violet LED Irradiation
description	Oral microbiome dysbiosis has important links to human health and disease. Although photodynamic therapy influences microbiome diversity, the specific effect of violet light irradiation remains largely unknown. In this study, we analyzed the effect of violet light-emitting diode (LED) irradiation on interdental plaque microbiota. Interdental plaque was collected from 12 human subjects, exposed to violet LED irradiation, and cultured in a specialized growth medium. Next-generation sequencing of the 16S ribosomal RNA genes revealed that α-diversity decreased, whereas β-diversity exhibited a continuous change with violet LED irradiation doses. In addition, we identified several operational taxonomic units that exhibited significant shifts during violet LED irradiation. Specifically, violet LED irradiation led to a significant reduction in the relative abundance of <i>Fusobacterium</i> species, but a significant increase in several species of oral bacteria, such as <i>Veillonella</i> and <i>Campylobacter</i>. Our study provides an overview of oral plaque microbiota changes under violet LED irradiation, and highlights the potential of this method for adjusting the balance of the oral microbiome without inducing antibiotic resistance.
format	article
author	Dan Wang Takayuki Nambu Hiroaki Tanimoto Naohiro Iwata Kazushi Yoshikawa Toshinori Okinaga Kazuyo Yamamoto
author_facet	Dan Wang Takayuki Nambu Hiroaki Tanimoto Naohiro Iwata Kazushi Yoshikawa Toshinori Okinaga Kazuyo Yamamoto
author_sort	Dan Wang
title	Interdental Plaque Microbial Community Changes under In Vitro Violet LED Irradiation
title_short	Interdental Plaque Microbial Community Changes under In Vitro Violet LED Irradiation
title_full	Interdental Plaque Microbial Community Changes under In Vitro Violet LED Irradiation
title_fullStr	Interdental Plaque Microbial Community Changes under In Vitro Violet LED Irradiation
title_full_unstemmed	Interdental Plaque Microbial Community Changes under In Vitro Violet LED Irradiation
title_sort	interdental plaque microbial community changes under in vitro violet led irradiation
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/38e0bb091fcd4231b50a70c767fe8dc3
work_keys_str_mv	AT danwang interdentalplaquemicrobialcommunitychangesunderinvitrovioletledirradiation AT takayukinambu interdentalplaquemicrobialcommunitychangesunderinvitrovioletledirradiation AT hiroakitanimoto interdentalplaquemicrobialcommunitychangesunderinvitrovioletledirradiation AT naohiroiwata interdentalplaquemicrobialcommunitychangesunderinvitrovioletledirradiation AT kazushiyoshikawa interdentalplaquemicrobialcommunitychangesunderinvitrovioletledirradiation AT toshinoriokinaga interdentalplaquemicrobialcommunitychangesunderinvitrovioletledirradiation AT kazuyoyamamoto interdentalplaquemicrobialcommunitychangesunderinvitrovioletledirradiation
_version_	1718413192947826688

Interdental Plaque Microbial Community Changes under In Vitro Violet LED Irradiation

Ejemplares similares