Effects Of PPARγ2 Pro12Ala Variant On Adipocyte Phenotype Dependent Of DHA

Renhui Wan,* Zhengping Ding,* Sheng Xia, Longyi Zheng, Jin Lu Department of Endocrinology, Changhai Hospital, Second Military Medical University, Shanghai 200433, People’s Republic of China*These authors contributed equally to this workCorrespondence: Jin Lu; Longyi ZhengDepartment of Endo...

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Autores principales:	Wan R, Ding Z, Xia S, Zheng L, Lu J
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2019
Materias:	peroxisome proliferator-activated receptor γ2 pro12ala variant docosahexaenoic acid adipocyte differentiation adiponectin Specialties of internal medicine RC581-951
Acceso en línea:	https://doaj.org/article/38e6097ab7ac42f199ee98216f87e97f
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spelling	oai:doaj.org-article:38e6097ab7ac42f199ee98216f87e97f2021-12-02T07:48:52ZEffects Of PPARγ2 Pro12Ala Variant On Adipocyte Phenotype Dependent Of DHA1178-7007https://doaj.org/article/38e6097ab7ac42f199ee98216f87e97f2019-11-01T00:00:00Zhttps://www.dovepress.com/effects-of-ppargamma2-pro12ala-variant-on-adipocyte-phenotype-dependen-peer-reviewed-article-DMSOhttps://doaj.org/toc/1178-7007Renhui Wan,* Zhengping Ding,* Sheng Xia, Longyi Zheng, Jin Lu Department of Endocrinology, Changhai Hospital, Second Military Medical University, Shanghai 200433, People’s Republic of China*These authors contributed equally to this workCorrespondence: Jin Lu; Longyi ZhengDepartment of Endocrinology, Changhai Hospital, Second Military Medical University, No.168, Changhai Road, Shanghai 200433, People’s Republic of ChinaEmail lujin-sh@139.com; longyizheng111@163.comBackground: Peroxisome proliferator-activated receptor γ2 (PPARγ2) plays a critical role in the regulation of adipocyte differentiation and adipocytokine production. The Pro12Ala variant is the most common mutation in the PPARγ2 gene. Its effect appears to be sensitive to dietary factors, such as docosahexaenoic acid (DHA) level. The purpose of this study was to investigate the interaction effect between PPARγ2 Pro12Ala variant and DHA on the phenotypes of adipocytes.Methods: We generated stable 3T3-L1 cell lines expressing wild-type PPARγ2 or PPARγ2 Pro12Ala variant. These two cell lines were cultured with different concentrations of DHA (0, 50, 200 umol/L). Then Oil red O staining was used to observe cell differentiation and the degree of lipid accumulation, TUNNEL assay was used to detect cell apoptosis, and ELISA assays were used to detect the changes of TNF-α, resistin and adiponectin levels in cell culture supernatant.Results: PPARγ2 Pro12Ala variant reduced lipid droplet accumulation in 3T3-L1 preadipocytes treated with or without 50 μmol/L DHA, but not with 200 μmol/L DHA, compared to that of wild-type PPARγ2. PPARγ2 reduced resistin production and increased adiponectin production in 3T3-L1 adipocytes, whereas PPARγ2 Pro12Ala variant diminished these effects. However, the absence of DHA blocked PPARγ2 Ala12 variant-induced effects on adiponectin production. There was no significant difference in TNF-α secretion between wild-type PPARγ2 and PPARγ2 Pro12Ala cells whether with or without DHA.Conclusion: These results indicated that the effects of PPARγ2 Pro12Ala variant were dependent on DHA concentration.Keywords: peroxisome proliferator-activated receptor γ2, Pro12Ala variant, docosahexaenoic acid, adipocyte, differentiation, adiponectinWan RDing ZXia SZheng LLu JDove Medical Pressarticleperoxisome proliferator-activated receptor γ2pro12ala variantdocosahexaenoic acidadipocytedifferentiationadiponectinSpecialties of internal medicineRC581-951ENDiabetes, Metabolic Syndrome and Obesity: Targets and Therapy, Vol Volume 12, Pp 2273-2279 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	peroxisome proliferator-activated receptor γ2 pro12ala variant docosahexaenoic acid adipocyte differentiation adiponectin Specialties of internal medicine RC581-951
spellingShingle	peroxisome proliferator-activated receptor γ2 pro12ala variant docosahexaenoic acid adipocyte differentiation adiponectin Specialties of internal medicine RC581-951 Wan R Ding Z Xia S Zheng L Lu J Effects Of PPARγ2 Pro12Ala Variant On Adipocyte Phenotype Dependent Of DHA
description	Renhui Wan,* Zhengping Ding,* Sheng Xia, Longyi Zheng, Jin Lu Department of Endocrinology, Changhai Hospital, Second Military Medical University, Shanghai 200433, People’s Republic of China*These authors contributed equally to this workCorrespondence: Jin Lu; Longyi ZhengDepartment of Endocrinology, Changhai Hospital, Second Military Medical University, No.168, Changhai Road, Shanghai 200433, People’s Republic of ChinaEmail lujin-sh@139.com; longyizheng111@163.comBackground: Peroxisome proliferator-activated receptor γ2 (PPARγ2) plays a critical role in the regulation of adipocyte differentiation and adipocytokine production. The Pro12Ala variant is the most common mutation in the PPARγ2 gene. Its effect appears to be sensitive to dietary factors, such as docosahexaenoic acid (DHA) level. The purpose of this study was to investigate the interaction effect between PPARγ2 Pro12Ala variant and DHA on the phenotypes of adipocytes.Methods: We generated stable 3T3-L1 cell lines expressing wild-type PPARγ2 or PPARγ2 Pro12Ala variant. These two cell lines were cultured with different concentrations of DHA (0, 50, 200 umol/L). Then Oil red O staining was used to observe cell differentiation and the degree of lipid accumulation, TUNNEL assay was used to detect cell apoptosis, and ELISA assays were used to detect the changes of TNF-α, resistin and adiponectin levels in cell culture supernatant.Results: PPARγ2 Pro12Ala variant reduced lipid droplet accumulation in 3T3-L1 preadipocytes treated with or without 50 μmol/L DHA, but not with 200 μmol/L DHA, compared to that of wild-type PPARγ2. PPARγ2 reduced resistin production and increased adiponectin production in 3T3-L1 adipocytes, whereas PPARγ2 Pro12Ala variant diminished these effects. However, the absence of DHA blocked PPARγ2 Ala12 variant-induced effects on adiponectin production. There was no significant difference in TNF-α secretion between wild-type PPARγ2 and PPARγ2 Pro12Ala cells whether with or without DHA.Conclusion: These results indicated that the effects of PPARγ2 Pro12Ala variant were dependent on DHA concentration.Keywords: peroxisome proliferator-activated receptor γ2, Pro12Ala variant, docosahexaenoic acid, adipocyte, differentiation, adiponectin
format	article
author	Wan R Ding Z Xia S Zheng L Lu J
author_facet	Wan R Ding Z Xia S Zheng L Lu J
author_sort	Wan R
title	Effects Of PPARγ2 Pro12Ala Variant On Adipocyte Phenotype Dependent Of DHA
title_short	Effects Of PPARγ2 Pro12Ala Variant On Adipocyte Phenotype Dependent Of DHA
title_full	Effects Of PPARγ2 Pro12Ala Variant On Adipocyte Phenotype Dependent Of DHA
title_fullStr	Effects Of PPARγ2 Pro12Ala Variant On Adipocyte Phenotype Dependent Of DHA
title_full_unstemmed	Effects Of PPARγ2 Pro12Ala Variant On Adipocyte Phenotype Dependent Of DHA
title_sort	effects of pparγ2 pro12ala variant on adipocyte phenotype dependent of dha
publisher	Dove Medical Press
publishDate	2019
url	https://doaj.org/article/38e6097ab7ac42f199ee98216f87e97f
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_version_	1718399149419790336

Effects Of PPARγ2 Pro12Ala Variant On Adipocyte Phenotype Dependent Of DHA

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