Rapid on-the-spot detection of Edwardsiella piscicida using recombinase polymerase amplification with lateral flow

Edwardsiella piscicida is one of the important pathogens in aquaculture, which takes huge losses to the economic benefits of turbot industry. Thus, a simple, rapid and accurate diagnostic assay of E. piscicida becomes an urgent need. In the present study, a detection method of E. piscicida based on...

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Autores principales:	Jiatiao Jiang, Yuhang Fan, Sijie Zhang, Qiyao Wang, Yuanxing Zhang, Qin Liu, Shuai Shao
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2022
Materias:	Edwardsiella piscicida Recombinase polymerase amplification Lateral flow On-the-spot detection Aquaculture. Fisheries. Angling SH1-691
Acceso en línea:	https://doaj.org/article/38f08f0c061c4071bb0289deffff1fd6
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spelling	oai:doaj.org-article:38f08f0c061c4071bb0289deffff1fd62021-11-16T04:10:50ZRapid on-the-spot detection of Edwardsiella piscicida using recombinase polymerase amplification with lateral flow2352-513410.1016/j.aqrep.2021.100945https://doaj.org/article/38f08f0c061c4071bb0289deffff1fd62022-02-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S2352513421003628https://doaj.org/toc/2352-5134Edwardsiella piscicida is one of the important pathogens in aquaculture, which takes huge losses to the economic benefits of turbot industry. Thus, a simple, rapid and accurate diagnostic assay of E. piscicida becomes an urgent need. In the present study, a detection method of E. piscicida based on recombinase polymerase amplification combined with lateral flow strip (RPA-LF) was established. The RPA-LF assay targeted E. piscicida evpP gene and could detect as low as 10 fg DNA of E. piscicida, which was approximately 1000 times more sensitive than conventional PCR assay. Meanwhile, the RPA-LF assay showed no cross-reactivity with DNA from other major bacterial pathogens in aquaculture. Besides, to improve the convenience of RPA-LF assay, a rapid on-the-spot sample preparation using nucleic acid extraction by cellulose dipsticks was established. Results indicated that bacteria could be detected as low as 1 copy and completed within 30 min. Moreover, this modified RPA-LF assay could be used to monitor E. piscicida infection in turbot industry.Jiatiao JiangYuhang FanSijie ZhangQiyao WangYuanxing ZhangQin LiuShuai ShaoElsevierarticleEdwardsiella piscicidaRecombinase polymerase amplificationLateral flowOn-the-spot detectionAquaculture. Fisheries. AnglingSH1-691ENAquaculture Reports, Vol 22, Iss , Pp 100945- (2022)
institution	DOAJ
collection	DOAJ
language	EN
topic	Edwardsiella piscicida Recombinase polymerase amplification Lateral flow On-the-spot detection Aquaculture. Fisheries. Angling SH1-691
spellingShingle	Edwardsiella piscicida Recombinase polymerase amplification Lateral flow On-the-spot detection Aquaculture. Fisheries. Angling SH1-691 Jiatiao Jiang Yuhang Fan Sijie Zhang Qiyao Wang Yuanxing Zhang Qin Liu Shuai Shao Rapid on-the-spot detection of Edwardsiella piscicida using recombinase polymerase amplification with lateral flow
description	Edwardsiella piscicida is one of the important pathogens in aquaculture, which takes huge losses to the economic benefits of turbot industry. Thus, a simple, rapid and accurate diagnostic assay of E. piscicida becomes an urgent need. In the present study, a detection method of E. piscicida based on recombinase polymerase amplification combined with lateral flow strip (RPA-LF) was established. The RPA-LF assay targeted E. piscicida evpP gene and could detect as low as 10 fg DNA of E. piscicida, which was approximately 1000 times more sensitive than conventional PCR assay. Meanwhile, the RPA-LF assay showed no cross-reactivity with DNA from other major bacterial pathogens in aquaculture. Besides, to improve the convenience of RPA-LF assay, a rapid on-the-spot sample preparation using nucleic acid extraction by cellulose dipsticks was established. Results indicated that bacteria could be detected as low as 1 copy and completed within 30 min. Moreover, this modified RPA-LF assay could be used to monitor E. piscicida infection in turbot industry.
format	article
author	Jiatiao Jiang Yuhang Fan Sijie Zhang Qiyao Wang Yuanxing Zhang Qin Liu Shuai Shao
author_facet	Jiatiao Jiang Yuhang Fan Sijie Zhang Qiyao Wang Yuanxing Zhang Qin Liu Shuai Shao
author_sort	Jiatiao Jiang
title	Rapid on-the-spot detection of Edwardsiella piscicida using recombinase polymerase amplification with lateral flow
title_short	Rapid on-the-spot detection of Edwardsiella piscicida using recombinase polymerase amplification with lateral flow
title_full	Rapid on-the-spot detection of Edwardsiella piscicida using recombinase polymerase amplification with lateral flow
title_fullStr	Rapid on-the-spot detection of Edwardsiella piscicida using recombinase polymerase amplification with lateral flow
title_full_unstemmed	Rapid on-the-spot detection of Edwardsiella piscicida using recombinase polymerase amplification with lateral flow
title_sort	rapid on-the-spot detection of edwardsiella piscicida using recombinase polymerase amplification with lateral flow
publisher	Elsevier
publishDate	2022
url	https://doaj.org/article/38f08f0c061c4071bb0289deffff1fd6
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Rapid on-the-spot detection of Edwardsiella piscicida using recombinase polymerase amplification with lateral flow

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