An imaging approach for determining the mechanism of enhancement of intestinal absorption of an L-theanine supplement.

<h4>Background & objective</h4>Theanine (L-glutamylethylamide) contained in green tea is a functional food component that has been attracting attention due to its relaxation effect. It was shown that the ingredients added to the theanine formulations increased the absorption of thean...

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Autores principales:	Yuki Sato, Kazuki Yamaguchi, Mikako Ogawa, Yoh Takekuma, Mitsuru Sugawara
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/38f43b4a19994eff938f8a8a369fd91e
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spelling	oai:doaj.org-article:38f43b4a19994eff938f8a8a369fd91e2021-12-02T20:10:43ZAn imaging approach for determining the mechanism of enhancement of intestinal absorption of an L-theanine supplement.1932-620310.1371/journal.pone.0253066https://doaj.org/article/38f43b4a19994eff938f8a8a369fd91e2021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0253066https://doaj.org/toc/1932-6203<h4>Background & objective</h4>Theanine (L-glutamylethylamide) contained in green tea is a functional food component that has been attracting attention due to its relaxation effect. It was shown that the ingredients added to the theanine formulations increased the absorption of theanine. If this mechanism can be elucidated, it would be possible to contribute to development of evidence-based formulations. In this study, we investigated the effect of ingredients in the formulations on the absorption of theanine in detail.<h4>Main methods</h4>After oral administration of a mixture of theanine and additional components to Wistar rats the plasma concentration was determined by an HPLC and the pharmacokinetic parameters were calculated. In addition, a new system for evaluating intestinal blood flow was developed since the involvement of intestinal blood flow was considered as a factor that increased absorption of theanine.<h4>Key findings</h4>Plasma concentration of theanine increased significantly in the combined use group with eight ingredients containing piperine as compared with theanine only group. Piperine would increase theanine absorption by increased blood flow, not an inhibition of metabolism. We succeeded to develop a visual and quantitative system to evaluate the effect of these ingredients directly including piperine on the intestinal blood flow using indocyanine green while maintaining physiological conditions.<h4>Significance</h4>Increased intestinal blood flow by these ingredients including piperine enhanced the absorption of theanine. Other mechanisms may also be considered as the mechanism by which theanine absorption is increased in addition to increased blood flow.Yuki SatoKazuki YamaguchiMikako OgawaYoh TakekumaMitsuru SugawaraPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 6, p e0253066 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Yuki Sato Kazuki Yamaguchi Mikako Ogawa Yoh Takekuma Mitsuru Sugawara An imaging approach for determining the mechanism of enhancement of intestinal absorption of an L-theanine supplement.
description	<h4>Background & objective</h4>Theanine (L-glutamylethylamide) contained in green tea is a functional food component that has been attracting attention due to its relaxation effect. It was shown that the ingredients added to the theanine formulations increased the absorption of theanine. If this mechanism can be elucidated, it would be possible to contribute to development of evidence-based formulations. In this study, we investigated the effect of ingredients in the formulations on the absorption of theanine in detail.<h4>Main methods</h4>After oral administration of a mixture of theanine and additional components to Wistar rats the plasma concentration was determined by an HPLC and the pharmacokinetic parameters were calculated. In addition, a new system for evaluating intestinal blood flow was developed since the involvement of intestinal blood flow was considered as a factor that increased absorption of theanine.<h4>Key findings</h4>Plasma concentration of theanine increased significantly in the combined use group with eight ingredients containing piperine as compared with theanine only group. Piperine would increase theanine absorption by increased blood flow, not an inhibition of metabolism. We succeeded to develop a visual and quantitative system to evaluate the effect of these ingredients directly including piperine on the intestinal blood flow using indocyanine green while maintaining physiological conditions.<h4>Significance</h4>Increased intestinal blood flow by these ingredients including piperine enhanced the absorption of theanine. Other mechanisms may also be considered as the mechanism by which theanine absorption is increased in addition to increased blood flow.
format	article
author	Yuki Sato Kazuki Yamaguchi Mikako Ogawa Yoh Takekuma Mitsuru Sugawara
author_facet	Yuki Sato Kazuki Yamaguchi Mikako Ogawa Yoh Takekuma Mitsuru Sugawara
author_sort	Yuki Sato
title	An imaging approach for determining the mechanism of enhancement of intestinal absorption of an L-theanine supplement.
title_short	An imaging approach for determining the mechanism of enhancement of intestinal absorption of an L-theanine supplement.
title_full	An imaging approach for determining the mechanism of enhancement of intestinal absorption of an L-theanine supplement.
title_fullStr	An imaging approach for determining the mechanism of enhancement of intestinal absorption of an L-theanine supplement.
title_full_unstemmed	An imaging approach for determining the mechanism of enhancement of intestinal absorption of an L-theanine supplement.
title_sort	imaging approach for determining the mechanism of enhancement of intestinal absorption of an l-theanine supplement.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/38f43b4a19994eff938f8a8a369fd91e
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An imaging approach for determining the mechanism of enhancement of intestinal absorption of an L-theanine supplement.

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