Transmembrane topology and oligomeric nature of an astrocytic membrane protein, MLC1

MLC1 is a membrane protein mainly expressed in astrocytes, and genetic mutations lead to the development of a leukodystrophy, megalencephalic leukoencephalopathy with subcortical cysts disease. Currently, the biochemical properties of the MLC1 protein are largely unknown. In this study, we aimed to...

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Autores principales:	Junmo Hwang, Kunwoong Park, Ga-Young Lee, Bo Young Yoon, Hyunmin Kim, Sung Hoon Roh, Byoung-Cheol Lee, Kipom Kim, Hyun-Ho Lim
Formato:	article
Lenguaje:	EN
Publicado:	The Royal Society 2021
Materias:	MLC1 membrane protein transmembrane topology subunit stoichiometry fluorescence quenching Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/38f4b72b65bd4330b9ced569bc801818
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spelling	oai:doaj.org-article:38f4b72b65bd4330b9ced569bc8018182021-12-01T08:06:04ZTransmembrane topology and oligomeric nature of an astrocytic membrane protein, MLC110.1098/rsob.2101032046-2441https://doaj.org/article/38f4b72b65bd4330b9ced569bc8018182021-12-01T00:00:00Zhttps://royalsocietypublishing.org/doi/10.1098/rsob.210103https://doaj.org/toc/2046-2441MLC1 is a membrane protein mainly expressed in astrocytes, and genetic mutations lead to the development of a leukodystrophy, megalencephalic leukoencephalopathy with subcortical cysts disease. Currently, the biochemical properties of the MLC1 protein are largely unknown. In this study, we aimed to characterize the transmembrane (TM) topology and oligomeric nature of the MLC1 protein. Systematic immunofluorescence staining data revealed that the MLC1 protein has eight TM domains and that both the N- and C-terminus face the cytoplasm. We found that MLC1 can be purified as an oligomer and could form a trimeric complex in both detergent micelles and reconstituted proteoliposomes. Additionally, a single-molecule photobleaching experiment showed that MLC1 protein complexes could consist of three MLC1 monomers in the reconstituted proteoliposomes. These results can provide a basis for both the high-resolution structural determination and functional characterization of the MLC1 protein.Junmo HwangKunwoong ParkGa-Young LeeBo Young YoonHyunmin KimSung Hoon RohByoung-Cheol LeeKipom KimHyun-Ho LimThe Royal SocietyarticleMLC1membrane proteintransmembrane topologysubunit stoichiometryfluorescence quenchingBiology (General)QH301-705.5ENOpen Biology, Vol 11, Iss 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	MLC1 membrane protein transmembrane topology subunit stoichiometry fluorescence quenching Biology (General) QH301-705.5
spellingShingle	MLC1 membrane protein transmembrane topology subunit stoichiometry fluorescence quenching Biology (General) QH301-705.5 Junmo Hwang Kunwoong Park Ga-Young Lee Bo Young Yoon Hyunmin Kim Sung Hoon Roh Byoung-Cheol Lee Kipom Kim Hyun-Ho Lim Transmembrane topology and oligomeric nature of an astrocytic membrane protein, MLC1
description	MLC1 is a membrane protein mainly expressed in astrocytes, and genetic mutations lead to the development of a leukodystrophy, megalencephalic leukoencephalopathy with subcortical cysts disease. Currently, the biochemical properties of the MLC1 protein are largely unknown. In this study, we aimed to characterize the transmembrane (TM) topology and oligomeric nature of the MLC1 protein. Systematic immunofluorescence staining data revealed that the MLC1 protein has eight TM domains and that both the N- and C-terminus face the cytoplasm. We found that MLC1 can be purified as an oligomer and could form a trimeric complex in both detergent micelles and reconstituted proteoliposomes. Additionally, a single-molecule photobleaching experiment showed that MLC1 protein complexes could consist of three MLC1 monomers in the reconstituted proteoliposomes. These results can provide a basis for both the high-resolution structural determination and functional characterization of the MLC1 protein.
format	article
author	Junmo Hwang Kunwoong Park Ga-Young Lee Bo Young Yoon Hyunmin Kim Sung Hoon Roh Byoung-Cheol Lee Kipom Kim Hyun-Ho Lim
author_facet	Junmo Hwang Kunwoong Park Ga-Young Lee Bo Young Yoon Hyunmin Kim Sung Hoon Roh Byoung-Cheol Lee Kipom Kim Hyun-Ho Lim
author_sort	Junmo Hwang
title	Transmembrane topology and oligomeric nature of an astrocytic membrane protein, MLC1
title_short	Transmembrane topology and oligomeric nature of an astrocytic membrane protein, MLC1
title_full	Transmembrane topology and oligomeric nature of an astrocytic membrane protein, MLC1
title_fullStr	Transmembrane topology and oligomeric nature of an astrocytic membrane protein, MLC1
title_full_unstemmed	Transmembrane topology and oligomeric nature of an astrocytic membrane protein, MLC1
title_sort	transmembrane topology and oligomeric nature of an astrocytic membrane protein, mlc1
publisher	The Royal Society
publishDate	2021
url	https://doaj.org/article/38f4b72b65bd4330b9ced569bc801818
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Transmembrane topology and oligomeric nature of an astrocytic membrane protein, MLC1

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