Effect of unacylated ghrelin on peripheral nerve regeneration

Ghrelin is a circulating peptide hormone released by enteroendocrine cells of the gastrointestinal tract as two forms, acylated and unacylated. Acylated ghrelin (AG) binds to the growth hormone secretagogue receptor 1a (GHSR1a), thus stimulating food intake, growth hormone release, and gastrointest...

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Autores principales:	Giulia Ronchi, Pierluigi Tos, Elia Angelino, Luisa Muratori, Simone Reano, Nicoletta Filigheddu, Andrea Graziani, Stefano Geuna, Stefania Raimondo
Formato:	article
Lenguaje:	EN
Publicado:	PAGEPress Publications 2021
Materias:	Ghrelin peripheral nerve regeneration functional recovery morphometry Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/38f9f3c4b50e4b2c8ed66a6f0ef0b4dd
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spelling	oai:doaj.org-article:38f9f3c4b50e4b2c8ed66a6f0ef0b4dd2021-11-05T07:36:07ZEffect of unacylated ghrelin on peripheral nerve regeneration10.4081/ejh.2021.32871121-760X2038-8306https://doaj.org/article/38f9f3c4b50e4b2c8ed66a6f0ef0b4dd2021-11-01T00:00:00Zhttps://www.ejh.it/index.php/ejh/article/view/3287https://doaj.org/toc/1121-760Xhttps://doaj.org/toc/2038-8306 Ghrelin is a circulating peptide hormone released by enteroendocrine cells of the gastrointestinal tract as two forms, acylated and unacylated. Acylated ghrelin (AG) binds to the growth hormone secretagogue receptor 1a (GHSR1a), thus stimulating food intake, growth hormone release, and gastrointestinal motility. Conversely, unacylated GHR (UnAG), through binding to a yet unidentified receptor, protects the skeletal muscle from atrophy, stimulates muscle regeneration, and protects cardiomyocytes from ischemic damage. Recently, interest about ghrelin has raised also among neuroscientists because of its effect on the nervous system, especially the stimulation of neurogenesis in spinal cord, brain stem, and hippocampus. However, few information is still available about its effectiveness on peripheral nerve regeneration. To partially fill this gap, the aim of this study was to assess the effect of UnAG on peripheral nerve regeneration after median nerve crush injury and after nerve transection immediately repaired by means of an end-to-end suture. To this end, we exploited FVB1 Myh6/Ghrl transgenic mice in which overexpression of the ghrelin gene (Ghrl) results in selective up-regulation of circulating UnAG levels, but not of AG. Regeneration was assessed by both functional evaluation (grasping test) and morphometrical analysis of regenerated myelinated axons. Results obtained lead to conclude that UnAG could have a role in development of peripheral nerves and during more severe lesions. Giulia RonchiPierluigi TosElia AngelinoLuisa MuratoriSimone ReanoNicoletta FilighedduAndrea GrazianiStefano GeunaStefania RaimondoPAGEPress PublicationsarticleGhrelinperipheral nerveregenerationfunctional recoverymorphometryBiology (General)QH301-705.5ENEuropean Journal of Histochemistry , Vol 65, Iss s1 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Ghrelin peripheral nerve regeneration functional recovery morphometry Biology (General) QH301-705.5
spellingShingle	Ghrelin peripheral nerve regeneration functional recovery morphometry Biology (General) QH301-705.5 Giulia Ronchi Pierluigi Tos Elia Angelino Luisa Muratori Simone Reano Nicoletta Filigheddu Andrea Graziani Stefano Geuna Stefania Raimondo Effect of unacylated ghrelin on peripheral nerve regeneration
description	Ghrelin is a circulating peptide hormone released by enteroendocrine cells of the gastrointestinal tract as two forms, acylated and unacylated. Acylated ghrelin (AG) binds to the growth hormone secretagogue receptor 1a (GHSR1a), thus stimulating food intake, growth hormone release, and gastrointestinal motility. Conversely, unacylated GHR (UnAG), through binding to a yet unidentified receptor, protects the skeletal muscle from atrophy, stimulates muscle regeneration, and protects cardiomyocytes from ischemic damage. Recently, interest about ghrelin has raised also among neuroscientists because of its effect on the nervous system, especially the stimulation of neurogenesis in spinal cord, brain stem, and hippocampus. However, few information is still available about its effectiveness on peripheral nerve regeneration. To partially fill this gap, the aim of this study was to assess the effect of UnAG on peripheral nerve regeneration after median nerve crush injury and after nerve transection immediately repaired by means of an end-to-end suture. To this end, we exploited FVB1 Myh6/Ghrl transgenic mice in which overexpression of the ghrelin gene (Ghrl) results in selective up-regulation of circulating UnAG levels, but not of AG. Regeneration was assessed by both functional evaluation (grasping test) and morphometrical analysis of regenerated myelinated axons. Results obtained lead to conclude that UnAG could have a role in development of peripheral nerves and during more severe lesions.
format	article
author	Giulia Ronchi Pierluigi Tos Elia Angelino Luisa Muratori Simone Reano Nicoletta Filigheddu Andrea Graziani Stefano Geuna Stefania Raimondo
author_facet	Giulia Ronchi Pierluigi Tos Elia Angelino Luisa Muratori Simone Reano Nicoletta Filigheddu Andrea Graziani Stefano Geuna Stefania Raimondo
author_sort	Giulia Ronchi
title	Effect of unacylated ghrelin on peripheral nerve regeneration
title_short	Effect of unacylated ghrelin on peripheral nerve regeneration
title_full	Effect of unacylated ghrelin on peripheral nerve regeneration
title_fullStr	Effect of unacylated ghrelin on peripheral nerve regeneration
title_full_unstemmed	Effect of unacylated ghrelin on peripheral nerve regeneration
title_sort	effect of unacylated ghrelin on peripheral nerve regeneration
publisher	PAGEPress Publications
publishDate	2021
url	https://doaj.org/article/38f9f3c4b50e4b2c8ed66a6f0ef0b4dd
work_keys_str_mv	AT giuliaronchi effectofunacylatedghrelinonperipheralnerveregeneration AT pierluigitos effectofunacylatedghrelinonperipheralnerveregeneration AT eliaangelino effectofunacylatedghrelinonperipheralnerveregeneration AT luisamuratori effectofunacylatedghrelinonperipheralnerveregeneration AT simonereano effectofunacylatedghrelinonperipheralnerveregeneration AT nicolettafiligheddu effectofunacylatedghrelinonperipheralnerveregeneration AT andreagraziani effectofunacylatedghrelinonperipheralnerveregeneration AT stefanogeuna effectofunacylatedghrelinonperipheralnerveregeneration AT stefaniaraimondo effectofunacylatedghrelinonperipheralnerveregeneration
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Effect of unacylated ghrelin on peripheral nerve regeneration

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