MutMap+: genetic mapping and mutant identification without crossing in rice.
Advances in genome sequencing technologies have enabled researchers and breeders to rapidly associate phenotypic variation to genome sequence differences. We recently took advantage of next-generation sequencing technology to develop MutMap, a method that allows rapid identification of causal nucleo...
Guardado en:
| Autores principales: | , , , , , , , , , , , , , , , , , , , |
|---|---|
| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
Public Library of Science (PLoS)
2013
|
| Materias: | |
| Acceso en línea: | https://doaj.org/article/3903090dc35e487abca9df36cdf02101 |
| Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
| id |
oai:doaj.org-article:3903090dc35e487abca9df36cdf02101 |
|---|---|
| record_format |
dspace |
| spelling |
oai:doaj.org-article:3903090dc35e487abca9df36cdf021012021-11-18T07:37:56ZMutMap+: genetic mapping and mutant identification without crossing in rice.1932-620310.1371/journal.pone.0068529https://doaj.org/article/3903090dc35e487abca9df36cdf021012013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23874658/?tool=EBIhttps://doaj.org/toc/1932-6203Advances in genome sequencing technologies have enabled researchers and breeders to rapidly associate phenotypic variation to genome sequence differences. We recently took advantage of next-generation sequencing technology to develop MutMap, a method that allows rapid identification of causal nucleotide changes of rice mutants by whole genome resequencing of pooled DNA of mutant F2 progeny derived from crosses made between candidate mutants and the parental line. Here we describe MutMap+, a versatile extension of MutMap, that identifies causal mutations by comparing SNP frequencies of bulked DNA of mutant and wild-type progeny of M3 generation derived from selfing of an M2 heterozygous individual. Notably, MutMap+ does not necessitate artificial crossing between mutants and the wild-type parental line. This method is therefore suitable for identifying mutations that cause early development lethality, sterility, or generally hamper crossing. Furthermore, MutMap+ is potentially useful for gene isolation in crops that are recalcitrant to artificial crosses.Rym FekihHiroki TakagiMuluneh TamiruAkira AbeSatoshi NatsumeHiroki YaegashiShailendra SharmaShiveta SharmaHiroyuki KanzakiHideo MatsumuraHiromasa SaitohChikako MitsuokaHiroe UtsushiAiko UemuraEiko KanzakiShunichi KosugiKentaro YoshidaLiliana CanoSophien KamounRyohei TerauchiPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 7, p e68529 (2013) |
| institution |
DOAJ |
| collection |
DOAJ |
| language |
EN |
| topic |
Medicine R Science Q |
| spellingShingle |
Medicine R Science Q Rym Fekih Hiroki Takagi Muluneh Tamiru Akira Abe Satoshi Natsume Hiroki Yaegashi Shailendra Sharma Shiveta Sharma Hiroyuki Kanzaki Hideo Matsumura Hiromasa Saitoh Chikako Mitsuoka Hiroe Utsushi Aiko Uemura Eiko Kanzaki Shunichi Kosugi Kentaro Yoshida Liliana Cano Sophien Kamoun Ryohei Terauchi MutMap+: genetic mapping and mutant identification without crossing in rice. |
| description |
Advances in genome sequencing technologies have enabled researchers and breeders to rapidly associate phenotypic variation to genome sequence differences. We recently took advantage of next-generation sequencing technology to develop MutMap, a method that allows rapid identification of causal nucleotide changes of rice mutants by whole genome resequencing of pooled DNA of mutant F2 progeny derived from crosses made between candidate mutants and the parental line. Here we describe MutMap+, a versatile extension of MutMap, that identifies causal mutations by comparing SNP frequencies of bulked DNA of mutant and wild-type progeny of M3 generation derived from selfing of an M2 heterozygous individual. Notably, MutMap+ does not necessitate artificial crossing between mutants and the wild-type parental line. This method is therefore suitable for identifying mutations that cause early development lethality, sterility, or generally hamper crossing. Furthermore, MutMap+ is potentially useful for gene isolation in crops that are recalcitrant to artificial crosses. |
| format |
article |
| author |
Rym Fekih Hiroki Takagi Muluneh Tamiru Akira Abe Satoshi Natsume Hiroki Yaegashi Shailendra Sharma Shiveta Sharma Hiroyuki Kanzaki Hideo Matsumura Hiromasa Saitoh Chikako Mitsuoka Hiroe Utsushi Aiko Uemura Eiko Kanzaki Shunichi Kosugi Kentaro Yoshida Liliana Cano Sophien Kamoun Ryohei Terauchi |
| author_facet |
Rym Fekih Hiroki Takagi Muluneh Tamiru Akira Abe Satoshi Natsume Hiroki Yaegashi Shailendra Sharma Shiveta Sharma Hiroyuki Kanzaki Hideo Matsumura Hiromasa Saitoh Chikako Mitsuoka Hiroe Utsushi Aiko Uemura Eiko Kanzaki Shunichi Kosugi Kentaro Yoshida Liliana Cano Sophien Kamoun Ryohei Terauchi |
| author_sort |
Rym Fekih |
| title |
MutMap+: genetic mapping and mutant identification without crossing in rice. |
| title_short |
MutMap+: genetic mapping and mutant identification without crossing in rice. |
| title_full |
MutMap+: genetic mapping and mutant identification without crossing in rice. |
| title_fullStr |
MutMap+: genetic mapping and mutant identification without crossing in rice. |
| title_full_unstemmed |
MutMap+: genetic mapping and mutant identification without crossing in rice. |
| title_sort |
mutmap+: genetic mapping and mutant identification without crossing in rice. |
| publisher |
Public Library of Science (PLoS) |
| publishDate |
2013 |
| url |
https://doaj.org/article/3903090dc35e487abca9df36cdf02101 |
| work_keys_str_mv |
AT rymfekih mutmapgeneticmappingandmutantidentificationwithoutcrossinginrice AT hirokitakagi mutmapgeneticmappingandmutantidentificationwithoutcrossinginrice AT mulunehtamiru mutmapgeneticmappingandmutantidentificationwithoutcrossinginrice AT akiraabe mutmapgeneticmappingandmutantidentificationwithoutcrossinginrice AT satoshinatsume mutmapgeneticmappingandmutantidentificationwithoutcrossinginrice AT hirokiyaegashi mutmapgeneticmappingandmutantidentificationwithoutcrossinginrice AT shailendrasharma mutmapgeneticmappingandmutantidentificationwithoutcrossinginrice AT shivetasharma mutmapgeneticmappingandmutantidentificationwithoutcrossinginrice AT hiroyukikanzaki mutmapgeneticmappingandmutantidentificationwithoutcrossinginrice AT hideomatsumura mutmapgeneticmappingandmutantidentificationwithoutcrossinginrice AT hiromasasaitoh mutmapgeneticmappingandmutantidentificationwithoutcrossinginrice AT chikakomitsuoka mutmapgeneticmappingandmutantidentificationwithoutcrossinginrice AT hiroeutsushi mutmapgeneticmappingandmutantidentificationwithoutcrossinginrice AT aikouemura mutmapgeneticmappingandmutantidentificationwithoutcrossinginrice AT eikokanzaki mutmapgeneticmappingandmutantidentificationwithoutcrossinginrice AT shunichikosugi mutmapgeneticmappingandmutantidentificationwithoutcrossinginrice AT kentaroyoshida mutmapgeneticmappingandmutantidentificationwithoutcrossinginrice AT lilianacano mutmapgeneticmappingandmutantidentificationwithoutcrossinginrice AT sophienkamoun mutmapgeneticmappingandmutantidentificationwithoutcrossinginrice AT ryoheiterauchi mutmapgeneticmappingandmutantidentificationwithoutcrossinginrice |
| _version_ |
1718423152579575808 |