Improved split fluorescent proteins for endogenous protein labeling

Split fluorescent proteins (FPs) have been widely used to visualise proteins in cells. Here the authors develop a screen for engineering new split FPs, and report a yellow-green split-mNeonGreen2 with reduced background, a red split-sfCherry2 for multicolour labeling, and its photoactivatable varian...

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Autores principales:	Siyu Feng, Sayaka Sekine, Veronica Pessino, Han Li, Manuel D. Leonetti, Bo Huang
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/39038b14682f4493a2f63d570fa4a7c6
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spelling	oai:doaj.org-article:39038b14682f4493a2f63d570fa4a7c62021-12-02T17:06:24ZImproved split fluorescent proteins for endogenous protein labeling10.1038/s41467-017-00494-82041-1723https://doaj.org/article/39038b14682f4493a2f63d570fa4a7c62017-08-01T00:00:00Zhttps://doi.org/10.1038/s41467-017-00494-8https://doaj.org/toc/2041-1723Split fluorescent proteins (FPs) have been widely used to visualise proteins in cells. Here the authors develop a screen for engineering new split FPs, and report a yellow-green split-mNeonGreen2 with reduced background, a red split-sfCherry2 for multicolour labeling, and its photoactivatable variant for super-resolution use.Siyu FengSayaka SekineVeronica PessinoHan LiManuel D. LeonettiBo HuangNature PortfolioarticleScienceQENNature Communications, Vol 8, Iss 1, Pp 1-11 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Siyu Feng Sayaka Sekine Veronica Pessino Han Li Manuel D. Leonetti Bo Huang Improved split fluorescent proteins for endogenous protein labeling
description	Split fluorescent proteins (FPs) have been widely used to visualise proteins in cells. Here the authors develop a screen for engineering new split FPs, and report a yellow-green split-mNeonGreen2 with reduced background, a red split-sfCherry2 for multicolour labeling, and its photoactivatable variant for super-resolution use.
format	article
author	Siyu Feng Sayaka Sekine Veronica Pessino Han Li Manuel D. Leonetti Bo Huang
author_facet	Siyu Feng Sayaka Sekine Veronica Pessino Han Li Manuel D. Leonetti Bo Huang
author_sort	Siyu Feng
title	Improved split fluorescent proteins for endogenous protein labeling
title_short	Improved split fluorescent proteins for endogenous protein labeling
title_full	Improved split fluorescent proteins for endogenous protein labeling
title_fullStr	Improved split fluorescent proteins for endogenous protein labeling
title_full_unstemmed	Improved split fluorescent proteins for endogenous protein labeling
title_sort	improved split fluorescent proteins for endogenous protein labeling
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/39038b14682f4493a2f63d570fa4a7c6
work_keys_str_mv	AT siyufeng improvedsplitfluorescentproteinsforendogenousproteinlabeling AT sayakasekine improvedsplitfluorescentproteinsforendogenousproteinlabeling AT veronicapessino improvedsplitfluorescentproteinsforendogenousproteinlabeling AT hanli improvedsplitfluorescentproteinsforendogenousproteinlabeling AT manueldleonetti improvedsplitfluorescentproteinsforendogenousproteinlabeling AT bohuang improvedsplitfluorescentproteinsforendogenousproteinlabeling
_version_	1718381606017695744

Improved split fluorescent proteins for endogenous protein labeling

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