Detection of PrPres in genetically susceptible fetuses from sheep with natural scrapie.

Scrapie is a transmissible spongiform encephalopathy with a wide PrPres dissemination in many non-neural tissues and with high levels of transmissibility within susceptible populations. Mechanisms of transmission are incompletely understood. It is generally assumed that it is horizontally transmitte...

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Autores principales:	María Carmen Garza, Natalia Fernández-Borges, Rosa Bolea, Juan José Badiola, Joaquín Castilla, Eva Monleón
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2011
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/390539be3ed14992b797fb1dcd722fb2
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spelling	oai:doaj.org-article:390539be3ed14992b797fb1dcd722fb22021-11-18T07:32:24ZDetection of PrPres in genetically susceptible fetuses from sheep with natural scrapie.1932-620310.1371/journal.pone.0027525https://doaj.org/article/390539be3ed14992b797fb1dcd722fb22011-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22194786/?tool=EBIhttps://doaj.org/toc/1932-6203Scrapie is a transmissible spongiform encephalopathy with a wide PrPres dissemination in many non-neural tissues and with high levels of transmissibility within susceptible populations. Mechanisms of transmission are incompletely understood. It is generally assumed that it is horizontally transmitted by direct contact between animals or indirectly through the environment, where scrapie can remain infectious for years. In contrast, in utero vertical transmission has never been demonstrated and has rarely been studied. Recently, the use of the protein misfolding cyclic amplification technique (PMCA) has allowed prion detection in various tissues and excretions in which PrPres levels have been undetectable by traditional assays. The main goal of this study was to detect PrPres in fetal tissues and the amniotic fluid from natural scrapie infected ewes using the PMCA technique. Six fetuses from three infected pregnant ewes in an advanced clinical stage of the disease were included in the study. From each fetus, amniotic fluid, brain, spleen, ileo-cecal valve and retropharyngeal lymph node samples were collected and analyzed using Western blotting and PMCA. Although all samples were negative using Western blotting, PrPres was detected after in vitro amplification. Our results represent the first time the biochemical detection of prions in fetal tissues, suggesting that the in utero transmission of scrapie in natural infected sheep might be possible.María Carmen GarzaNatalia Fernández-BorgesRosa BoleaJuan José BadiolaJoaquín CastillaEva MonleónPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 6, Iss 12, p e27525 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q María Carmen Garza Natalia Fernández-Borges Rosa Bolea Juan José Badiola Joaquín Castilla Eva Monleón Detection of PrPres in genetically susceptible fetuses from sheep with natural scrapie.
description	Scrapie is a transmissible spongiform encephalopathy with a wide PrPres dissemination in many non-neural tissues and with high levels of transmissibility within susceptible populations. Mechanisms of transmission are incompletely understood. It is generally assumed that it is horizontally transmitted by direct contact between animals or indirectly through the environment, where scrapie can remain infectious for years. In contrast, in utero vertical transmission has never been demonstrated and has rarely been studied. Recently, the use of the protein misfolding cyclic amplification technique (PMCA) has allowed prion detection in various tissues and excretions in which PrPres levels have been undetectable by traditional assays. The main goal of this study was to detect PrPres in fetal tissues and the amniotic fluid from natural scrapie infected ewes using the PMCA technique. Six fetuses from three infected pregnant ewes in an advanced clinical stage of the disease were included in the study. From each fetus, amniotic fluid, brain, spleen, ileo-cecal valve and retropharyngeal lymph node samples were collected and analyzed using Western blotting and PMCA. Although all samples were negative using Western blotting, PrPres was detected after in vitro amplification. Our results represent the first time the biochemical detection of prions in fetal tissues, suggesting that the in utero transmission of scrapie in natural infected sheep might be possible.
format	article
author	María Carmen Garza Natalia Fernández-Borges Rosa Bolea Juan José Badiola Joaquín Castilla Eva Monleón
author_facet	María Carmen Garza Natalia Fernández-Borges Rosa Bolea Juan José Badiola Joaquín Castilla Eva Monleón
author_sort	María Carmen Garza
title	Detection of PrPres in genetically susceptible fetuses from sheep with natural scrapie.
title_short	Detection of PrPres in genetically susceptible fetuses from sheep with natural scrapie.
title_full	Detection of PrPres in genetically susceptible fetuses from sheep with natural scrapie.
title_fullStr	Detection of PrPres in genetically susceptible fetuses from sheep with natural scrapie.
title_full_unstemmed	Detection of PrPres in genetically susceptible fetuses from sheep with natural scrapie.
title_sort	detection of prpres in genetically susceptible fetuses from sheep with natural scrapie.
publisher	Public Library of Science (PLoS)
publishDate	2011
url	https://doaj.org/article/390539be3ed14992b797fb1dcd722fb2
work_keys_str_mv	AT mariacarmengarza detectionofprpresingeneticallysusceptiblefetusesfromsheepwithnaturalscrapie AT nataliafernandezborges detectionofprpresingeneticallysusceptiblefetusesfromsheepwithnaturalscrapie AT rosabolea detectionofprpresingeneticallysusceptiblefetusesfromsheepwithnaturalscrapie AT juanjosebadiola detectionofprpresingeneticallysusceptiblefetusesfromsheepwithnaturalscrapie AT joaquincastilla detectionofprpresingeneticallysusceptiblefetusesfromsheepwithnaturalscrapie AT evamonleon detectionofprpresingeneticallysusceptiblefetusesfromsheepwithnaturalscrapie
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Detection of PrPres in genetically susceptible fetuses from sheep with natural scrapie.

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