Crosstalk between Helicobacter pylori and gastric epithelial cells is impaired by docosahexaenoic acid.
H. pylori colonizes half of the world's population leading to gastritis, ulcers and gastric cancer. H. pylori strains resistant to antibiotics are increasing which raises the need for alternative therapeutic approaches. Docosahexaenoic acid (DHA) has been shown to decrease H. pylori growth and...
Guardado en:
| Autores principales: | , , , , , , , , , , , , |
|---|---|
| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
Public Library of Science (PLoS)
2013
|
| Materias: | |
| Acceso en línea: | https://doaj.org/article/390620e051e24262a8e32b28a9a7426d |
| Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
| id |
oai:doaj.org-article:390620e051e24262a8e32b28a9a7426d |
|---|---|
| record_format |
dspace |
| spelling |
oai:doaj.org-article:390620e051e24262a8e32b28a9a7426d2021-11-18T07:50:25ZCrosstalk between Helicobacter pylori and gastric epithelial cells is impaired by docosahexaenoic acid.1932-620310.1371/journal.pone.0060657https://doaj.org/article/390620e051e24262a8e32b28a9a7426d2013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23577140/?tool=EBIhttps://doaj.org/toc/1932-6203H. pylori colonizes half of the world's population leading to gastritis, ulcers and gastric cancer. H. pylori strains resistant to antibiotics are increasing which raises the need for alternative therapeutic approaches. Docosahexaenoic acid (DHA) has been shown to decrease H. pylori growth and its associated-inflammation through mechanisms poorly characterized. We aimed to explore DHA action on H. pylori-mediated inflammation and adhesion to gastric epithelial cells (AGS) and also to identify bacterial structures affected by DHA. H. pylori growth and metabolism was assessed in liquid cultures. Bacterial adhesion to AGS cells was visualized by transmission electron microscopy and quantified by an Enzyme Linked Immunosorbent Assay. Inflammatory proteins were assessed by immunoblotting in infected AGS cells, previously treated with DHA. Bacterial total and outer membrane protein composition was analyzed by 2-dimensional gel electrophoresis. Concentrations of 100 µM of DHA decreased H. pylori growth, whereas concentrations higher than 250 µM irreversibly inhibited bacteria survival. DHA reduced ATP production and adhesion to AGS cells. AGS cells infected with DHA pre-treated H. pylori showed a 3-fold reduction in Interleukin-8 (IL-8) production and a decrease of COX2 and iNOS. 2D electrophoresis analysis revealed that DHA changed the expression of H. pylori outer membrane proteins associated with stress response and metabolism and modified bacterial lipopolysaccharide phenotype. As conclusions our results show that DHA anti-H. pylori effects are associated with changes of bacteria morphology and metabolism, and with alteration of outer membrane proteins composition, that ultimately reduce the adhesion of bacteria and the burden of H. pylori-related inflammation.Marta CorreiaValérie MichelHugo OsórioMeriem El GhachiMathilde BonisIvo G BonecaHilde De ReuseAntónio A MatosPascal LenormandRaquel SerucaCeu FigueiredoJose Carlos MachadoEliette TouatiPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 4, p e60657 (2013) |
| institution |
DOAJ |
| collection |
DOAJ |
| language |
EN |
| topic |
Medicine R Science Q |
| spellingShingle |
Medicine R Science Q Marta Correia Valérie Michel Hugo Osório Meriem El Ghachi Mathilde Bonis Ivo G Boneca Hilde De Reuse António A Matos Pascal Lenormand Raquel Seruca Ceu Figueiredo Jose Carlos Machado Eliette Touati Crosstalk between Helicobacter pylori and gastric epithelial cells is impaired by docosahexaenoic acid. |
| description |
H. pylori colonizes half of the world's population leading to gastritis, ulcers and gastric cancer. H. pylori strains resistant to antibiotics are increasing which raises the need for alternative therapeutic approaches. Docosahexaenoic acid (DHA) has been shown to decrease H. pylori growth and its associated-inflammation through mechanisms poorly characterized. We aimed to explore DHA action on H. pylori-mediated inflammation and adhesion to gastric epithelial cells (AGS) and also to identify bacterial structures affected by DHA. H. pylori growth and metabolism was assessed in liquid cultures. Bacterial adhesion to AGS cells was visualized by transmission electron microscopy and quantified by an Enzyme Linked Immunosorbent Assay. Inflammatory proteins were assessed by immunoblotting in infected AGS cells, previously treated with DHA. Bacterial total and outer membrane protein composition was analyzed by 2-dimensional gel electrophoresis. Concentrations of 100 µM of DHA decreased H. pylori growth, whereas concentrations higher than 250 µM irreversibly inhibited bacteria survival. DHA reduced ATP production and adhesion to AGS cells. AGS cells infected with DHA pre-treated H. pylori showed a 3-fold reduction in Interleukin-8 (IL-8) production and a decrease of COX2 and iNOS. 2D electrophoresis analysis revealed that DHA changed the expression of H. pylori outer membrane proteins associated with stress response and metabolism and modified bacterial lipopolysaccharide phenotype. As conclusions our results show that DHA anti-H. pylori effects are associated with changes of bacteria morphology and metabolism, and with alteration of outer membrane proteins composition, that ultimately reduce the adhesion of bacteria and the burden of H. pylori-related inflammation. |
| format |
article |
| author |
Marta Correia Valérie Michel Hugo Osório Meriem El Ghachi Mathilde Bonis Ivo G Boneca Hilde De Reuse António A Matos Pascal Lenormand Raquel Seruca Ceu Figueiredo Jose Carlos Machado Eliette Touati |
| author_facet |
Marta Correia Valérie Michel Hugo Osório Meriem El Ghachi Mathilde Bonis Ivo G Boneca Hilde De Reuse António A Matos Pascal Lenormand Raquel Seruca Ceu Figueiredo Jose Carlos Machado Eliette Touati |
| author_sort |
Marta Correia |
| title |
Crosstalk between Helicobacter pylori and gastric epithelial cells is impaired by docosahexaenoic acid. |
| title_short |
Crosstalk between Helicobacter pylori and gastric epithelial cells is impaired by docosahexaenoic acid. |
| title_full |
Crosstalk between Helicobacter pylori and gastric epithelial cells is impaired by docosahexaenoic acid. |
| title_fullStr |
Crosstalk between Helicobacter pylori and gastric epithelial cells is impaired by docosahexaenoic acid. |
| title_full_unstemmed |
Crosstalk between Helicobacter pylori and gastric epithelial cells is impaired by docosahexaenoic acid. |
| title_sort |
crosstalk between helicobacter pylori and gastric epithelial cells is impaired by docosahexaenoic acid. |
| publisher |
Public Library of Science (PLoS) |
| publishDate |
2013 |
| url |
https://doaj.org/article/390620e051e24262a8e32b28a9a7426d |
| work_keys_str_mv |
AT martacorreia crosstalkbetweenhelicobacterpyloriandgastricepithelialcellsisimpairedbydocosahexaenoicacid AT valeriemichel crosstalkbetweenhelicobacterpyloriandgastricepithelialcellsisimpairedbydocosahexaenoicacid AT hugoosorio crosstalkbetweenhelicobacterpyloriandgastricepithelialcellsisimpairedbydocosahexaenoicacid AT meriemelghachi crosstalkbetweenhelicobacterpyloriandgastricepithelialcellsisimpairedbydocosahexaenoicacid AT mathildebonis crosstalkbetweenhelicobacterpyloriandgastricepithelialcellsisimpairedbydocosahexaenoicacid AT ivogboneca crosstalkbetweenhelicobacterpyloriandgastricepithelialcellsisimpairedbydocosahexaenoicacid AT hildedereuse crosstalkbetweenhelicobacterpyloriandgastricepithelialcellsisimpairedbydocosahexaenoicacid AT antonioamatos crosstalkbetweenhelicobacterpyloriandgastricepithelialcellsisimpairedbydocosahexaenoicacid AT pascallenormand crosstalkbetweenhelicobacterpyloriandgastricepithelialcellsisimpairedbydocosahexaenoicacid AT raquelseruca crosstalkbetweenhelicobacterpyloriandgastricepithelialcellsisimpairedbydocosahexaenoicacid AT ceufigueiredo crosstalkbetweenhelicobacterpyloriandgastricepithelialcellsisimpairedbydocosahexaenoicacid AT josecarlosmachado crosstalkbetweenhelicobacterpyloriandgastricepithelialcellsisimpairedbydocosahexaenoicacid AT eliettetouati crosstalkbetweenhelicobacterpyloriandgastricepithelialcellsisimpairedbydocosahexaenoicacid |
| _version_ |
1718422844201762816 |