Nanoparticles containing allotropes of carbon have genotoxic effects on glioblastomamultiforme cells

Mateusz Hinzmann,1 Slawomir Jaworski,1 Marta Kutwin,1 Joanna Jagiello,2 Rafal Kozinski,2 Mateusz Wierzbicki,1 Marta Grodzik,1 Ludwika Lipinska,2 Ewa Sawosz,1 Andrè Chwalibog31Division of Nanobiotechnology, Warsaw University of Life Sciences, 2Institute of Electronic Materials Technology,...

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Autores principales: Hinzmann M, Jaworski S, Kutwin M, Jagiełło J, Koziński R, Wierzbicki M, Grodzik M, Lipińska L, Sawosz E, Chwalibog A
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Publicado: Dove Medical Press 2014
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spelling oai:doaj.org-article:39119bf1848848a8bb8e6a662e3e6ba02021-12-02T07:44:04ZNanoparticles containing allotropes of carbon have genotoxic effects on glioblastomamultiforme cells1178-2013https://doaj.org/article/39119bf1848848a8bb8e6a662e3e6ba02014-05-01T00:00:00Zhttp://www.dovepress.com/nanoparticles-containing-allotropes-of-carbon-have-genotoxic-effects-o-a16861https://doaj.org/toc/1178-2013 Mateusz Hinzmann,1 Slawomir Jaworski,1 Marta Kutwin,1 Joanna Jagiello,2 Rafal Kozinski,2 Mateusz Wierzbicki,1 Marta Grodzik,1 Ludwika Lipinska,2 Ewa Sawosz,1 Andrè Chwalibog31Division of Nanobiotechnology, Warsaw University of Life Sciences, 2Institute of Electronic Materials Technology, Warsaw, Poland; 3Department of Veterinary Clinical and Animal Sciences, University of Copenhagen, Copenhagen, DenmarkAbstract: The carbon-based nanomaterial family consists of nanoparticles containing allotropes of carbon, which may have a number of interactions with biological systems. The objective of this study was to evaluate the toxicity of nanoparticles comprised of pristine graphene, reduced graphene oxide, graphene oxide, graphite, and ultradispersed detonation diamond in a U87 cell line. The scope of the work consisted of structural analysis of the nanoparticles using transmission electron microscopy, evaluation of cell morphology, and assessment of cell viability by Trypan blue assay and level of DNA fragmentation of U87 cells after 24 hours of incubation with 50 µg/mL carbon nanoparticles. DNA fragmentation was studied using single-cell gel electrophoresis. Incubation with nanoparticles containing the allotropes of carbon did not alter the morphology of the U87 cancer cells. However, incubation with pristine graphene and reduced graphene oxide led to a significant decrease in cell viability, whereas incubation with graphene oxide, graphite, and ultradispersed detonation diamond led to a smaller decrease in cell viability. The results of a comet assay demonstrated that pristine graphene, reduced graphene oxide, graphite, and ultradispersed detonation diamond caused DNA damage and were therefore genotoxic in U87 cells, whereas graphene oxide was not.Keywords: nanostructures, graphene, graphite, diamond, glioblastoma multiforme, geno toxicityHinzmann MJaworski SKutwin MJagiełło JKoziński RWierzbicki MGrodzik MLipińska LSawosz EChwalibog ADove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2014, Iss Issue 1, Pp 2409-2417 (2014)
institution DOAJ
collection DOAJ
language EN
topic Medicine (General)
R5-920
spellingShingle Medicine (General)
R5-920
Hinzmann M
Jaworski S
Kutwin M
Jagiełło J
Koziński R
Wierzbicki M
Grodzik M
Lipińska L
Sawosz E
Chwalibog A
Nanoparticles containing allotropes of carbon have genotoxic effects on glioblastomamultiforme cells
description Mateusz Hinzmann,1 Slawomir Jaworski,1 Marta Kutwin,1 Joanna Jagiello,2 Rafal Kozinski,2 Mateusz Wierzbicki,1 Marta Grodzik,1 Ludwika Lipinska,2 Ewa Sawosz,1 Andrè Chwalibog31Division of Nanobiotechnology, Warsaw University of Life Sciences, 2Institute of Electronic Materials Technology, Warsaw, Poland; 3Department of Veterinary Clinical and Animal Sciences, University of Copenhagen, Copenhagen, DenmarkAbstract: The carbon-based nanomaterial family consists of nanoparticles containing allotropes of carbon, which may have a number of interactions with biological systems. The objective of this study was to evaluate the toxicity of nanoparticles comprised of pristine graphene, reduced graphene oxide, graphene oxide, graphite, and ultradispersed detonation diamond in a U87 cell line. The scope of the work consisted of structural analysis of the nanoparticles using transmission electron microscopy, evaluation of cell morphology, and assessment of cell viability by Trypan blue assay and level of DNA fragmentation of U87 cells after 24 hours of incubation with 50 µg/mL carbon nanoparticles. DNA fragmentation was studied using single-cell gel electrophoresis. Incubation with nanoparticles containing the allotropes of carbon did not alter the morphology of the U87 cancer cells. However, incubation with pristine graphene and reduced graphene oxide led to a significant decrease in cell viability, whereas incubation with graphene oxide, graphite, and ultradispersed detonation diamond led to a smaller decrease in cell viability. The results of a comet assay demonstrated that pristine graphene, reduced graphene oxide, graphite, and ultradispersed detonation diamond caused DNA damage and were therefore genotoxic in U87 cells, whereas graphene oxide was not.Keywords: nanostructures, graphene, graphite, diamond, glioblastoma multiforme, geno toxicity
format article
author Hinzmann M
Jaworski S
Kutwin M
Jagiełło J
Koziński R
Wierzbicki M
Grodzik M
Lipińska L
Sawosz E
Chwalibog A
author_facet Hinzmann M
Jaworski S
Kutwin M
Jagiełło J
Koziński R
Wierzbicki M
Grodzik M
Lipińska L
Sawosz E
Chwalibog A
author_sort Hinzmann M
title Nanoparticles containing allotropes of carbon have genotoxic effects on glioblastomamultiforme cells
title_short Nanoparticles containing allotropes of carbon have genotoxic effects on glioblastomamultiforme cells
title_full Nanoparticles containing allotropes of carbon have genotoxic effects on glioblastomamultiforme cells
title_fullStr Nanoparticles containing allotropes of carbon have genotoxic effects on glioblastomamultiforme cells
title_full_unstemmed Nanoparticles containing allotropes of carbon have genotoxic effects on glioblastomamultiforme cells
title_sort nanoparticles containing allotropes of carbon have genotoxic effects on glioblastomamultiforme cells
publisher Dove Medical Press
publishDate 2014
url https://doaj.org/article/39119bf1848848a8bb8e6a662e3e6ba0
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AT jagiełłoj nanoparticlescontainingallotropesofcarbonhavegenotoxiceffectsonglioblastomamultiformecells
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