Evaluation of seven gene signature for predicting HCV recurrence post-liver transplantation

Abstract Background Orthotropic liver transplantation (OLT) offers a therapeutic choice for hepatocellular carcinoma (HCC) patients. The poor outcome of liver transplantation is HCV recurrence. Several genome-wide associated studies (GWAS) have reported many genetic variants to be associated with HC...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Ghada M. Salum, Mai Abd el Meguid, Tawfeek H. Abelhafez, Eman Medhat, Ashraf O. Abdel Aziz, Reham Dawood
Formato:	article
Lenguaje:	EN
Publicado:	SpringerOpen 2021
Materias:	HCV CRS Donor steatosis Recipients Orthotropic liver transplantation SNP Biotechnology TP248.13-248.65 Genetics QH426-470
Acceso en línea:	https://doaj.org/article/3912f7441c4b4838bbcf21b1c872ebc4
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:3912f7441c4b4838bbcf21b1c872ebc4
record_format	dspace
spelling	oai:doaj.org-article:3912f7441c4b4838bbcf21b1c872ebc42021-11-14T12:32:58ZEvaluation of seven gene signature for predicting HCV recurrence post-liver transplantation10.1186/s43141-021-00266-42090-5920https://doaj.org/article/3912f7441c4b4838bbcf21b1c872ebc42021-11-01T00:00:00Zhttps://doi.org/10.1186/s43141-021-00266-4https://doaj.org/toc/2090-5920Abstract Background Orthotropic liver transplantation (OLT) offers a therapeutic choice for hepatocellular carcinoma (HCC) patients. The poor outcome of liver transplantation is HCV recurrence. Several genome-wide associated studies (GWAS) have reported many genetic variants to be associated with HCV recurrence. Seven gene polymorphisms formed a cirrhosis risk score (CRS) signature that could be used to distinguish chronic HCV patients at high risk from those at low risk for cirrhosis in non-transplant patients. This study aims to examine the association of CRS score and other clinical parameters with the probability for HCC emergence and/or the rate of HCV recurrence following liver transplantation. Results Seven gene polymorphisms, forming the CRS, were genotyped by real-time PCR using allelic discrimination protocol in 199 end-stage liver disease patients (79 child A, 43 child B, and 77child C), comprising 106 patients who encountered liver transplantation. Recipient CRS scores were correlated with HCV recurrence (HCV-Rec) at the end of the third year after OLT. Around 81% (39) recipients with low steatosis (LS; < 3.5%) donor percentage revealed no HCV recurrence (non-Rec) (p<0.001). CRS score could distinguish between child A, child B, and child C only at the low-risk group. Among the HCV Rec group 27% (8/30), 40% (12/30), and 33% (10/30) fell into the high, moderate, and low CRS risk groups, respectively. Stepwise logistic regression evinced two features more likely to be seen in HCV-Rec patients: abnormal ALT [OR, 1.1; 95% CI, 1.02–1.2] and donor steatosis >3.5% [OR, 46.07; 95% CI, 1.5–1407.8]. Conclusions Accordingly, the CRS score seems to be less useful to predict HCV recurrence after OLT. ALT and donor steatosis (exceed 3.5%) can significantly promote the HCV recurrence post-OLT. Moreover, the combination of MMF and CNI positively heightens HCV recurrence.Ghada M. SalumMai Abd el MeguidTawfeek H. AbelhafezEman MedhatAshraf O. Abdel AzizReham DawoodSpringerOpenarticleHCVCRSDonor steatosisRecipientsOrthotropic liver transplantationSNPBiotechnologyTP248.13-248.65GeneticsQH426-470ENJournal of Genetic Engineering and Biotechnology, Vol 19, Iss 1, Pp 1-10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	HCV CRS Donor steatosis Recipients Orthotropic liver transplantation SNP Biotechnology TP248.13-248.65 Genetics QH426-470
spellingShingle	HCV CRS Donor steatosis Recipients Orthotropic liver transplantation SNP Biotechnology TP248.13-248.65 Genetics QH426-470 Ghada M. Salum Mai Abd el Meguid Tawfeek H. Abelhafez Eman Medhat Ashraf O. Abdel Aziz Reham Dawood Evaluation of seven gene signature for predicting HCV recurrence post-liver transplantation
description	Abstract Background Orthotropic liver transplantation (OLT) offers a therapeutic choice for hepatocellular carcinoma (HCC) patients. The poor outcome of liver transplantation is HCV recurrence. Several genome-wide associated studies (GWAS) have reported many genetic variants to be associated with HCV recurrence. Seven gene polymorphisms formed a cirrhosis risk score (CRS) signature that could be used to distinguish chronic HCV patients at high risk from those at low risk for cirrhosis in non-transplant patients. This study aims to examine the association of CRS score and other clinical parameters with the probability for HCC emergence and/or the rate of HCV recurrence following liver transplantation. Results Seven gene polymorphisms, forming the CRS, were genotyped by real-time PCR using allelic discrimination protocol in 199 end-stage liver disease patients (79 child A, 43 child B, and 77child C), comprising 106 patients who encountered liver transplantation. Recipient CRS scores were correlated with HCV recurrence (HCV-Rec) at the end of the third year after OLT. Around 81% (39) recipients with low steatosis (LS; < 3.5%) donor percentage revealed no HCV recurrence (non-Rec) (p<0.001). CRS score could distinguish between child A, child B, and child C only at the low-risk group. Among the HCV Rec group 27% (8/30), 40% (12/30), and 33% (10/30) fell into the high, moderate, and low CRS risk groups, respectively. Stepwise logistic regression evinced two features more likely to be seen in HCV-Rec patients: abnormal ALT [OR, 1.1; 95% CI, 1.02–1.2] and donor steatosis >3.5% [OR, 46.07; 95% CI, 1.5–1407.8]. Conclusions Accordingly, the CRS score seems to be less useful to predict HCV recurrence after OLT. ALT and donor steatosis (exceed 3.5%) can significantly promote the HCV recurrence post-OLT. Moreover, the combination of MMF and CNI positively heightens HCV recurrence.
format	article
author	Ghada M. Salum Mai Abd el Meguid Tawfeek H. Abelhafez Eman Medhat Ashraf O. Abdel Aziz Reham Dawood
author_facet	Ghada M. Salum Mai Abd el Meguid Tawfeek H. Abelhafez Eman Medhat Ashraf O. Abdel Aziz Reham Dawood
author_sort	Ghada M. Salum
title	Evaluation of seven gene signature for predicting HCV recurrence post-liver transplantation
title_short	Evaluation of seven gene signature for predicting HCV recurrence post-liver transplantation
title_full	Evaluation of seven gene signature for predicting HCV recurrence post-liver transplantation
title_fullStr	Evaluation of seven gene signature for predicting HCV recurrence post-liver transplantation
title_full_unstemmed	Evaluation of seven gene signature for predicting HCV recurrence post-liver transplantation
title_sort	evaluation of seven gene signature for predicting hcv recurrence post-liver transplantation
publisher	SpringerOpen
publishDate	2021
url	https://doaj.org/article/3912f7441c4b4838bbcf21b1c872ebc4
work_keys_str_mv	AT ghadamsalum evaluationofsevengenesignatureforpredictinghcvrecurrencepostlivertransplantation AT maiabdelmeguid evaluationofsevengenesignatureforpredictinghcvrecurrencepostlivertransplantation AT tawfeekhabelhafez evaluationofsevengenesignatureforpredictinghcvrecurrencepostlivertransplantation AT emanmedhat evaluationofsevengenesignatureforpredictinghcvrecurrencepostlivertransplantation AT ashrafoabdelaziz evaluationofsevengenesignatureforpredictinghcvrecurrencepostlivertransplantation AT rehamdawood evaluationofsevengenesignatureforpredictinghcvrecurrencepostlivertransplantation
_version_	1718429160572977152

Evaluation of seven gene signature for predicting HCV recurrence post-liver transplantation

Ejemplares similares