Vitrification of particulated articular cartilage via calculated protocols

Abstract Preserving viable articular cartilage is a promising approach to address the shortage of graft tissue and enable the clinical repair of articular cartilage defects in articulating joints, such as the knee, ankle, and hip. In this study, we developed two 2-step, dual-temperature, multicryopr...

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Autores principales:	Kezhou Wu, Nadia Shardt, Leila Laouar, Janet A. W. Elliott, Nadr M. Jomha
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R
Acceso en línea:	https://doaj.org/article/39166fa9a73d45809d88af5bddf1cfa2
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spelling	oai:doaj.org-article:39166fa9a73d45809d88af5bddf1cfa22021-12-02T16:05:49ZVitrification of particulated articular cartilage via calculated protocols10.1038/s41536-021-00123-52057-3995https://doaj.org/article/39166fa9a73d45809d88af5bddf1cfa22021-03-01T00:00:00Zhttps://doi.org/10.1038/s41536-021-00123-5https://doaj.org/toc/2057-3995Abstract Preserving viable articular cartilage is a promising approach to address the shortage of graft tissue and enable the clinical repair of articular cartilage defects in articulating joints, such as the knee, ankle, and hip. In this study, we developed two 2-step, dual-temperature, multicryoprotectant loading protocols to cryopreserve particulated articular cartilage (cubes ~1 mm3 in size) using a mathematical approach, and we experimentally measured chondrocyte viability, metabolic activity, cell migration, and matrix productivity after implementing the designed loading protocols, vitrification, and warming. We demonstrated that porcine and human articular cartilage cubes can be successfully vitrified and rewarmed, maintaining high cell viability and excellent cellular function. The vitrified particulated articular cartilage was stored for a period of 6 months with no significant deterioration in chondrocyte viability and functionality. Our approach enables high-quality long-term storage of viable articular cartilage that can alleviate the shortage of grafts for use in clinically repairing articular cartilage defects.Kezhou WuNadia ShardtLeila LaouarJanet A. W. ElliottNadr M. JomhaNature PortfolioarticleMedicineRENnpj Regenerative Medicine, Vol 6, Iss 1, Pp 1-13 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R
spellingShingle	Medicine R Kezhou Wu Nadia Shardt Leila Laouar Janet A. W. Elliott Nadr M. Jomha Vitrification of particulated articular cartilage via calculated protocols
description	Abstract Preserving viable articular cartilage is a promising approach to address the shortage of graft tissue and enable the clinical repair of articular cartilage defects in articulating joints, such as the knee, ankle, and hip. In this study, we developed two 2-step, dual-temperature, multicryoprotectant loading protocols to cryopreserve particulated articular cartilage (cubes ~1 mm3 in size) using a mathematical approach, and we experimentally measured chondrocyte viability, metabolic activity, cell migration, and matrix productivity after implementing the designed loading protocols, vitrification, and warming. We demonstrated that porcine and human articular cartilage cubes can be successfully vitrified and rewarmed, maintaining high cell viability and excellent cellular function. The vitrified particulated articular cartilage was stored for a period of 6 months with no significant deterioration in chondrocyte viability and functionality. Our approach enables high-quality long-term storage of viable articular cartilage that can alleviate the shortage of grafts for use in clinically repairing articular cartilage defects.
format	article
author	Kezhou Wu Nadia Shardt Leila Laouar Janet A. W. Elliott Nadr M. Jomha
author_facet	Kezhou Wu Nadia Shardt Leila Laouar Janet A. W. Elliott Nadr M. Jomha
author_sort	Kezhou Wu
title	Vitrification of particulated articular cartilage via calculated protocols
title_short	Vitrification of particulated articular cartilage via calculated protocols
title_full	Vitrification of particulated articular cartilage via calculated protocols
title_fullStr	Vitrification of particulated articular cartilage via calculated protocols
title_full_unstemmed	Vitrification of particulated articular cartilage via calculated protocols
title_sort	vitrification of particulated articular cartilage via calculated protocols
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/39166fa9a73d45809d88af5bddf1cfa2
work_keys_str_mv	AT kezhouwu vitrificationofparticulatedarticularcartilageviacalculatedprotocols AT nadiashardt vitrificationofparticulatedarticularcartilageviacalculatedprotocols AT leilalaouar vitrificationofparticulatedarticularcartilageviacalculatedprotocols AT janetawelliott vitrificationofparticulatedarticularcartilageviacalculatedprotocols AT nadrmjomha vitrificationofparticulatedarticularcartilageviacalculatedprotocols
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Vitrification of particulated articular cartilage via calculated protocols

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