Salicylic acid is a key player of Arabidopsis autophagy mutant susceptibility to the necrotrophic bacterium Dickeya dadantii

Abstract Autophagy is a ubiquitous vesicular process for protein and organelle recycling in eukaryotes. In plant, autophagy is reported to play pivotal roles in nutrient recycling, adaptation to biotic and abiotic stresses. The role of autophagy in plant immunity remains poorly understood. Several r...

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Autores principales:	Martine Rigault, Sylvie Citerne, Céline Masclaux-Daubresse, Alia Dellagi
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/39181e5f560046fbb2591031f7f077c5
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spelling	oai:doaj.org-article:39181e5f560046fbb2591031f7f077c52021-12-02T14:26:54ZSalicylic acid is a key player of Arabidopsis autophagy mutant susceptibility to the necrotrophic bacterium Dickeya dadantii10.1038/s41598-021-83067-62045-2322https://doaj.org/article/39181e5f560046fbb2591031f7f077c52021-02-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-83067-6https://doaj.org/toc/2045-2322Abstract Autophagy is a ubiquitous vesicular process for protein and organelle recycling in eukaryotes. In plant, autophagy is reported to play pivotal roles in nutrient recycling, adaptation to biotic and abiotic stresses. The role of autophagy in plant immunity remains poorly understood. Several reports showed enhanced susceptibility of different Arabidopsis autophagy mutants (atg) to necrotrophic fungal pathogens. Interaction of necrotrophic bacterial pathogens with autophagy is overlooked. We then investigated such interaction by inoculating the necrotrophic enterobacterium Dickeya dadantii in leaves of the atg2 and atg5 mutants and an ATG8a overexpressing line. Overexpressing ATG8a enhances plant tolerance to D. dadantii. While atg5 mutant displayed similar susceptibility to the WT, the atg2 mutant exhibited accelerated leaf senescence and enhanced susceptibility upon infection. Both phenotypes were reversed when the sid2 mutation, abolishing SA signaling, was introduced in the atg2 mutant. High levels of SA signaling in atg2 mutant resulted in repression of the jasmonic acid (JA) defense pathway known to limit D. dadantii progression in A. thaliana. We provide evidence that in atg2 mutant, the disturbed hormonal balance leading to higher SA signaling is the main factor causing increased susceptibility to the D. dadantii necrotroph by repressing the JA pathway and accelerating developmental senescence.Martine RigaultSylvie CiterneCéline Masclaux-DaubresseAlia DellagiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Martine Rigault Sylvie Citerne Céline Masclaux-Daubresse Alia Dellagi Salicylic acid is a key player of Arabidopsis autophagy mutant susceptibility to the necrotrophic bacterium Dickeya dadantii
description	Abstract Autophagy is a ubiquitous vesicular process for protein and organelle recycling in eukaryotes. In plant, autophagy is reported to play pivotal roles in nutrient recycling, adaptation to biotic and abiotic stresses. The role of autophagy in plant immunity remains poorly understood. Several reports showed enhanced susceptibility of different Arabidopsis autophagy mutants (atg) to necrotrophic fungal pathogens. Interaction of necrotrophic bacterial pathogens with autophagy is overlooked. We then investigated such interaction by inoculating the necrotrophic enterobacterium Dickeya dadantii in leaves of the atg2 and atg5 mutants and an ATG8a overexpressing line. Overexpressing ATG8a enhances plant tolerance to D. dadantii. While atg5 mutant displayed similar susceptibility to the WT, the atg2 mutant exhibited accelerated leaf senescence and enhanced susceptibility upon infection. Both phenotypes were reversed when the sid2 mutation, abolishing SA signaling, was introduced in the atg2 mutant. High levels of SA signaling in atg2 mutant resulted in repression of the jasmonic acid (JA) defense pathway known to limit D. dadantii progression in A. thaliana. We provide evidence that in atg2 mutant, the disturbed hormonal balance leading to higher SA signaling is the main factor causing increased susceptibility to the D. dadantii necrotroph by repressing the JA pathway and accelerating developmental senescence.
format	article
author	Martine Rigault Sylvie Citerne Céline Masclaux-Daubresse Alia Dellagi
author_facet	Martine Rigault Sylvie Citerne Céline Masclaux-Daubresse Alia Dellagi
author_sort	Martine Rigault
title	Salicylic acid is a key player of Arabidopsis autophagy mutant susceptibility to the necrotrophic bacterium Dickeya dadantii
title_short	Salicylic acid is a key player of Arabidopsis autophagy mutant susceptibility to the necrotrophic bacterium Dickeya dadantii
title_full	Salicylic acid is a key player of Arabidopsis autophagy mutant susceptibility to the necrotrophic bacterium Dickeya dadantii
title_fullStr	Salicylic acid is a key player of Arabidopsis autophagy mutant susceptibility to the necrotrophic bacterium Dickeya dadantii
title_full_unstemmed	Salicylic acid is a key player of Arabidopsis autophagy mutant susceptibility to the necrotrophic bacterium Dickeya dadantii
title_sort	salicylic acid is a key player of arabidopsis autophagy mutant susceptibility to the necrotrophic bacterium dickeya dadantii
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/39181e5f560046fbb2591031f7f077c5
work_keys_str_mv	AT martinerigault salicylicacidisakeyplayerofarabidopsisautophagymutantsusceptibilitytothenecrotrophicbacteriumdickeyadadantii AT sylvieciterne salicylicacidisakeyplayerofarabidopsisautophagymutantsusceptibilitytothenecrotrophicbacteriumdickeyadadantii AT celinemasclauxdaubresse salicylicacidisakeyplayerofarabidopsisautophagymutantsusceptibilitytothenecrotrophicbacteriumdickeyadadantii AT aliadellagi salicylicacidisakeyplayerofarabidopsisautophagymutantsusceptibilitytothenecrotrophicbacteriumdickeyadadantii
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Salicylic acid is a key player of Arabidopsis autophagy mutant susceptibility to the necrotrophic bacterium Dickeya dadantii

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