Effect of AGM and fetal liver-derived stromal cell lines on globin expression in adult baboon (P. anubis) bone marrow-derived erythroid progenitors.

This study was performed to investigate the hypothesis that the erythroid micro-environment plays a role in regulation of globin gene expression during adult erythroid differentiation. Adult baboon bone marrow and human cord blood CD34+ progenitors were grown in methylcellulose, liquid media, and in...

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Autores principales:	Donald Lavelle, Kestutis Vaitkus, Maria Armila Ruiz, Vinzon Ibanez, Tatiana Kouznetsova, Yogen Saunthararajah, Nadim Mahmud, Joseph DeSimone
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/391de4e825e4450f8ddf210e93828059
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spelling	oai:doaj.org-article:391de4e825e4450f8ddf210e938280592021-11-18T07:16:44ZEffect of AGM and fetal liver-derived stromal cell lines on globin expression in adult baboon (P. anubis) bone marrow-derived erythroid progenitors.1932-620310.1371/journal.pone.0036846https://doaj.org/article/391de4e825e4450f8ddf210e938280592012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22693559/?tool=EBIhttps://doaj.org/toc/1932-6203This study was performed to investigate the hypothesis that the erythroid micro-environment plays a role in regulation of globin gene expression during adult erythroid differentiation. Adult baboon bone marrow and human cord blood CD34+ progenitors were grown in methylcellulose, liquid media, and in co-culture with stromal cell lines derived from different developmental stages in identical media supporting erythroid differentiation to examine the effect of the micro-environment on globin gene expression. Adult progenitors express high levels of γ-globin in liquid and methylcellulose media but low, physiological levels in stromal cell co-cultures. In contrast, γ-globin expression remained high in cord blood progenitors in stromal cell line co-cultures. Differences in γ-globin gene expression between adult progenitors in stromal cell line co-cultures and liquid media required cell-cell contact and were associated with differences in rate of differentiation and γ-globin promoter DNA methylation. We conclude that γ-globin expression in adult-derived erythroid cells can be influenced by the micro-environment, suggesting new potential targets for HbF induction.Donald LavelleKestutis VaitkusMaria Armila RuizVinzon IbanezTatiana KouznetsovaYogen SaunthararajahNadim MahmudJoseph DeSimonePublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 5, p e36846 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Donald Lavelle Kestutis Vaitkus Maria Armila Ruiz Vinzon Ibanez Tatiana Kouznetsova Yogen Saunthararajah Nadim Mahmud Joseph DeSimone Effect of AGM and fetal liver-derived stromal cell lines on globin expression in adult baboon (P. anubis) bone marrow-derived erythroid progenitors.
description	This study was performed to investigate the hypothesis that the erythroid micro-environment plays a role in regulation of globin gene expression during adult erythroid differentiation. Adult baboon bone marrow and human cord blood CD34+ progenitors were grown in methylcellulose, liquid media, and in co-culture with stromal cell lines derived from different developmental stages in identical media supporting erythroid differentiation to examine the effect of the micro-environment on globin gene expression. Adult progenitors express high levels of γ-globin in liquid and methylcellulose media but low, physiological levels in stromal cell co-cultures. In contrast, γ-globin expression remained high in cord blood progenitors in stromal cell line co-cultures. Differences in γ-globin gene expression between adult progenitors in stromal cell line co-cultures and liquid media required cell-cell contact and were associated with differences in rate of differentiation and γ-globin promoter DNA methylation. We conclude that γ-globin expression in adult-derived erythroid cells can be influenced by the micro-environment, suggesting new potential targets for HbF induction.
format	article
author	Donald Lavelle Kestutis Vaitkus Maria Armila Ruiz Vinzon Ibanez Tatiana Kouznetsova Yogen Saunthararajah Nadim Mahmud Joseph DeSimone
author_facet	Donald Lavelle Kestutis Vaitkus Maria Armila Ruiz Vinzon Ibanez Tatiana Kouznetsova Yogen Saunthararajah Nadim Mahmud Joseph DeSimone
author_sort	Donald Lavelle
title	Effect of AGM and fetal liver-derived stromal cell lines on globin expression in adult baboon (P. anubis) bone marrow-derived erythroid progenitors.
title_short	Effect of AGM and fetal liver-derived stromal cell lines on globin expression in adult baboon (P. anubis) bone marrow-derived erythroid progenitors.
title_full	Effect of AGM and fetal liver-derived stromal cell lines on globin expression in adult baboon (P. anubis) bone marrow-derived erythroid progenitors.
title_fullStr	Effect of AGM and fetal liver-derived stromal cell lines on globin expression in adult baboon (P. anubis) bone marrow-derived erythroid progenitors.
title_full_unstemmed	Effect of AGM and fetal liver-derived stromal cell lines on globin expression in adult baboon (P. anubis) bone marrow-derived erythroid progenitors.
title_sort	effect of agm and fetal liver-derived stromal cell lines on globin expression in adult baboon (p. anubis) bone marrow-derived erythroid progenitors.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/391de4e825e4450f8ddf210e93828059
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Effect of AGM and fetal liver-derived stromal cell lines on globin expression in adult baboon (P. anubis) bone marrow-derived erythroid progenitors.

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