Gene expression profiling reveals candidate biomarkers and probable molecular mechanism in diabetic peripheral neuropathy

Han Zhou, WenChuan ZhangDepartment of Neurosurgery, Ninth People Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai 200011, People’s Republic of ChinaPurpose: To investigate the molecular mechanism and search for candidate biomarkers in the gene expression pr...

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Autores principales:	Zhou H, Zhang W
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2019
Materias:	Differentially expressed genes functional enrichment analysis demyelination lipid metabolism CIDEC Specialties of internal medicine RC581-951
Acceso en línea:	https://doaj.org/article/392203ef508d454b8d91db97a4794fb9
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spelling	oai:doaj.org-article:392203ef508d454b8d91db97a4794fb92021-12-02T02:30:20ZGene expression profiling reveals candidate biomarkers and probable molecular mechanism in diabetic peripheral neuropathy1178-7007https://doaj.org/article/392203ef508d454b8d91db97a4794fb92019-07-01T00:00:00Zhttps://www.dovepress.com/gene-expression-profiling-reveals-candidate-biomarkers-and-probable-mo-peer-reviewed-article-DMSOhttps://doaj.org/toc/1178-7007Han Zhou, WenChuan ZhangDepartment of Neurosurgery, Ninth People Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai 200011, People’s Republic of ChinaPurpose: To investigate the molecular mechanism and search for candidate biomarkers in the gene expression profile of patients with diabetic peripheral neuropathy (DPN).Methods: Differentially expressed genes (DEGs) of progressive vs non-progressive DPN patients in dataset GSE24290 were screened. Functional enrichment analysis was conducted, and hub genes were extracted from the protein–protein interaction network. The expression level of hub genes in serum samples in another dataset GSE95849 was obtained, followed by the ROC curve analysis.Results: A total of 352 DEGs were obtained from dataset GSE24290. They were involved in 14 gene ontology terms and 10 Kyoto Encyclopedia of Genes and Genomes pathways, mainly related to lipid metabolism. Eight hub genes (LEP, APOE, ADIPOQ, FABP4, CD36, GPAM, CIDEC, and PNPLA4) were revealed, and their expression level was obtained in dataset GSE95849. The receiver operatingcharacteristic curve analysis indicated that CIDEC (AUC=1), APOE (AUC=0.833), CD36 (AUC=0.803), and PNPLA4 (AUC=0.861) might be candidate serum biomarkers of DPN.Conclusion: Lipid metabolism of Schwann cells might be inhibited in progressive DPN. CIDEC, APOE, CD36, and PNPLA4 might be potential predictive biomarkers in the early DPN diagnosis of patients with DM.Keywords: differentially expressed genes, functional enrichment analysis, demyelination, lipid metabolism, diabetic peripheral neuropathyZhou HZhang WDove Medical PressarticleDifferentially expressed genesfunctional enrichment analysisdemyelinationlipid metabolismCIDECSpecialties of internal medicineRC581-951ENDiabetes, Metabolic Syndrome and Obesity: Targets and Therapy, Vol Volume 12, Pp 1213-1223 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Differentially expressed genes functional enrichment analysis demyelination lipid metabolism CIDEC Specialties of internal medicine RC581-951
spellingShingle	Differentially expressed genes functional enrichment analysis demyelination lipid metabolism CIDEC Specialties of internal medicine RC581-951 Zhou H Zhang W Gene expression profiling reveals candidate biomarkers and probable molecular mechanism in diabetic peripheral neuropathy
description	Han Zhou, WenChuan ZhangDepartment of Neurosurgery, Ninth People Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai 200011, People’s Republic of ChinaPurpose: To investigate the molecular mechanism and search for candidate biomarkers in the gene expression profile of patients with diabetic peripheral neuropathy (DPN).Methods: Differentially expressed genes (DEGs) of progressive vs non-progressive DPN patients in dataset GSE24290 were screened. Functional enrichment analysis was conducted, and hub genes were extracted from the protein–protein interaction network. The expression level of hub genes in serum samples in another dataset GSE95849 was obtained, followed by the ROC curve analysis.Results: A total of 352 DEGs were obtained from dataset GSE24290. They were involved in 14 gene ontology terms and 10 Kyoto Encyclopedia of Genes and Genomes pathways, mainly related to lipid metabolism. Eight hub genes (LEP, APOE, ADIPOQ, FABP4, CD36, GPAM, CIDEC, and PNPLA4) were revealed, and their expression level was obtained in dataset GSE95849. The receiver operatingcharacteristic curve analysis indicated that CIDEC (AUC=1), APOE (AUC=0.833), CD36 (AUC=0.803), and PNPLA4 (AUC=0.861) might be candidate serum biomarkers of DPN.Conclusion: Lipid metabolism of Schwann cells might be inhibited in progressive DPN. CIDEC, APOE, CD36, and PNPLA4 might be potential predictive biomarkers in the early DPN diagnosis of patients with DM.Keywords: differentially expressed genes, functional enrichment analysis, demyelination, lipid metabolism, diabetic peripheral neuropathy
format	article
author	Zhou H Zhang W
author_facet	Zhou H Zhang W
author_sort	Zhou H
title	Gene expression profiling reveals candidate biomarkers and probable molecular mechanism in diabetic peripheral neuropathy
title_short	Gene expression profiling reveals candidate biomarkers and probable molecular mechanism in diabetic peripheral neuropathy
title_full	Gene expression profiling reveals candidate biomarkers and probable molecular mechanism in diabetic peripheral neuropathy
title_fullStr	Gene expression profiling reveals candidate biomarkers and probable molecular mechanism in diabetic peripheral neuropathy
title_full_unstemmed	Gene expression profiling reveals candidate biomarkers and probable molecular mechanism in diabetic peripheral neuropathy
title_sort	gene expression profiling reveals candidate biomarkers and probable molecular mechanism in diabetic peripheral neuropathy
publisher	Dove Medical Press
publishDate	2019
url	https://doaj.org/article/392203ef508d454b8d91db97a4794fb9
work_keys_str_mv	AT zhouh geneexpressionprofilingrevealscandidatebiomarkersandprobablemolecularmechanismindiabeticperipheralneuropathy AT zhangw geneexpressionprofilingrevealscandidatebiomarkersandprobablemolecularmechanismindiabeticperipheralneuropathy
_version_	1718402402167554048

Gene expression profiling reveals candidate biomarkers and probable molecular mechanism in diabetic peripheral neuropathy

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