Platelet behaviour on von Willebrand Factor changes in pregnancy: Consequences of haemodilution and intrinsic changes in platelet function

Abstract Platelet function in pregnancy is poorly understood. Previous studies of platelet function in pregnancy have used non-physiological assays of platelet function with conflicting results. This study using a physiological assay of platelet function investigated platelet interactions with von W...

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Autores principales:	Jonathan Cowman, Sieglinde Müllers, Eimear Dunne, Adam Ralph, Antonio J. Ricco, Fergal. D. Malone, Dermot Kenny
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/3923398470bb4b9bb8006a4ceed82e1a
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spelling	oai:doaj.org-article:3923398470bb4b9bb8006a4ceed82e1a2021-12-02T16:06:10ZPlatelet behaviour on von Willebrand Factor changes in pregnancy: Consequences of haemodilution and intrinsic changes in platelet function10.1038/s41598-017-06959-62045-2322https://doaj.org/article/3923398470bb4b9bb8006a4ceed82e1a2017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-06959-6https://doaj.org/toc/2045-2322Abstract Platelet function in pregnancy is poorly understood. Previous studies of platelet function in pregnancy have used non-physiological assays of platelet function with conflicting results. This study using a physiological assay of platelet function investigated platelet interactions with von Willebrand Factor (VWF) in blood from healthy pregnant women and healthy non-pregnant controls. Blood samples (200 µl) from third-trimester pregnancies (n = 21) and non-pregnant controls (n = 21) were perfused through custom-made parallel-plate flow chambers coated with VWF under arterial shear (1,500 s−1). Multi-parameter measurements of platelet interactions with the immobilized VWF surface were recorded by digital-image microscopy and analysed using custom-designed platelet-tracking software. Platelet interactions with VWF decreased in healthy third-trimester pregnant participants relative to controls. This effect is most likely due to haemodilution which occurs physiologically during pregnancy. Interestingly, platelets in blood from pregnant participants translocated more slowly on VWF under arterial-shear conditions. These decreases in platelet translocation speed were independent of haemodilution, suggesting intrinsic changes in platelet function with pregnancy.Jonathan CowmanSieglinde MüllersEimear DunneAdam RalphAntonio J. RiccoFergal. D. MaloneDermot KennyNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-7 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Jonathan Cowman Sieglinde Müllers Eimear Dunne Adam Ralph Antonio J. Ricco Fergal. D. Malone Dermot Kenny Platelet behaviour on von Willebrand Factor changes in pregnancy: Consequences of haemodilution and intrinsic changes in platelet function
description	Abstract Platelet function in pregnancy is poorly understood. Previous studies of platelet function in pregnancy have used non-physiological assays of platelet function with conflicting results. This study using a physiological assay of platelet function investigated platelet interactions with von Willebrand Factor (VWF) in blood from healthy pregnant women and healthy non-pregnant controls. Blood samples (200 µl) from third-trimester pregnancies (n = 21) and non-pregnant controls (n = 21) were perfused through custom-made parallel-plate flow chambers coated with VWF under arterial shear (1,500 s−1). Multi-parameter measurements of platelet interactions with the immobilized VWF surface were recorded by digital-image microscopy and analysed using custom-designed platelet-tracking software. Platelet interactions with VWF decreased in healthy third-trimester pregnant participants relative to controls. This effect is most likely due to haemodilution which occurs physiologically during pregnancy. Interestingly, platelets in blood from pregnant participants translocated more slowly on VWF under arterial-shear conditions. These decreases in platelet translocation speed were independent of haemodilution, suggesting intrinsic changes in platelet function with pregnancy.
format	article
author	Jonathan Cowman Sieglinde Müllers Eimear Dunne Adam Ralph Antonio J. Ricco Fergal. D. Malone Dermot Kenny
author_facet	Jonathan Cowman Sieglinde Müllers Eimear Dunne Adam Ralph Antonio J. Ricco Fergal. D. Malone Dermot Kenny
author_sort	Jonathan Cowman
title	Platelet behaviour on von Willebrand Factor changes in pregnancy: Consequences of haemodilution and intrinsic changes in platelet function
title_short	Platelet behaviour on von Willebrand Factor changes in pregnancy: Consequences of haemodilution and intrinsic changes in platelet function
title_full	Platelet behaviour on von Willebrand Factor changes in pregnancy: Consequences of haemodilution and intrinsic changes in platelet function
title_fullStr	Platelet behaviour on von Willebrand Factor changes in pregnancy: Consequences of haemodilution and intrinsic changes in platelet function
title_full_unstemmed	Platelet behaviour on von Willebrand Factor changes in pregnancy: Consequences of haemodilution and intrinsic changes in platelet function
title_sort	platelet behaviour on von willebrand factor changes in pregnancy: consequences of haemodilution and intrinsic changes in platelet function
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/3923398470bb4b9bb8006a4ceed82e1a
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Platelet behaviour on von Willebrand Factor changes in pregnancy: Consequences of haemodilution and intrinsic changes in platelet function

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