The role of retinol dehydrogenase 10 in the cone visual cycle

Abstract Pigment regeneration is critical for the function of cone photoreceptors in bright and rapidly-changing light conditions. This process is facilitated by the recently-characterized retina visual cycle, in which Müller cells recycle spent all-trans-retinol visual chromophore back to 11-cis-re...

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Autores principales:	Yunlu Xue, Shinya Sato, David Razafsky, Bhubanananda Sahu, Susan Q. Shen, Chloe Potter, Lisa L. Sandell, Joseph C. Corbo, Krzysztof Palczewski, Akiko Maeda, Didier Hodzic, Vladimir J. Kefalov
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/392513b8e5794336a8525df1ce0d8453
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spelling	oai:doaj.org-article:392513b8e5794336a8525df1ce0d84532021-12-02T15:06:22ZThe role of retinol dehydrogenase 10 in the cone visual cycle10.1038/s41598-017-02549-82045-2322https://doaj.org/article/392513b8e5794336a8525df1ce0d84532017-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-02549-8https://doaj.org/toc/2045-2322Abstract Pigment regeneration is critical for the function of cone photoreceptors in bright and rapidly-changing light conditions. This process is facilitated by the recently-characterized retina visual cycle, in which Müller cells recycle spent all-trans-retinol visual chromophore back to 11-cis-retinol. This 11-cis-retinol is oxidized selectively in cones to the 11-cis-retinal used for pigment regeneration. However, the enzyme responsible for the oxidation of 11-cis-retinol remains unknown. Here, we sought to determine whether retinol dehydrogenase 10 (RDH10), upregulated in rod/cone hybrid retinas and expressed abundantly in Müller cells, is the enzyme that drives this reaction. We created mice lacking RDH10 either in cone photoreceptors, Müller cells, or the entire retina. In vivo electroretinography and transretinal recordings revealed normal cone photoresponses in all RDH10-deficient mouse lines. Notably, their cone-driven dark adaptation both in vivo and in isolated retina was unaffected, indicating that RDH10 is not required for the function of the retina visual cycle. We also generated transgenic mice expressing RDH10 ectopically in rod cells. However, rod dark adaptation was unaffected by the expression of RDH10 and transgenic rods were unable to use cis-retinol for pigment regeneration. We conclude that RDH10 is not the dominant retina 11-cis-RDH, leaving its primary function in the retina unknown.Yunlu XueShinya SatoDavid RazafskyBhubanananda SahuSusan Q. ShenChloe PotterLisa L. SandellJoseph C. CorboKrzysztof PalczewskiAkiko MaedaDidier HodzicVladimir J. KefalovNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-13 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Yunlu Xue Shinya Sato David Razafsky Bhubanananda Sahu Susan Q. Shen Chloe Potter Lisa L. Sandell Joseph C. Corbo Krzysztof Palczewski Akiko Maeda Didier Hodzic Vladimir J. Kefalov The role of retinol dehydrogenase 10 in the cone visual cycle
description	Abstract Pigment regeneration is critical for the function of cone photoreceptors in bright and rapidly-changing light conditions. This process is facilitated by the recently-characterized retina visual cycle, in which Müller cells recycle spent all-trans-retinol visual chromophore back to 11-cis-retinol. This 11-cis-retinol is oxidized selectively in cones to the 11-cis-retinal used for pigment regeneration. However, the enzyme responsible for the oxidation of 11-cis-retinol remains unknown. Here, we sought to determine whether retinol dehydrogenase 10 (RDH10), upregulated in rod/cone hybrid retinas and expressed abundantly in Müller cells, is the enzyme that drives this reaction. We created mice lacking RDH10 either in cone photoreceptors, Müller cells, or the entire retina. In vivo electroretinography and transretinal recordings revealed normal cone photoresponses in all RDH10-deficient mouse lines. Notably, their cone-driven dark adaptation both in vivo and in isolated retina was unaffected, indicating that RDH10 is not required for the function of the retina visual cycle. We also generated transgenic mice expressing RDH10 ectopically in rod cells. However, rod dark adaptation was unaffected by the expression of RDH10 and transgenic rods were unable to use cis-retinol for pigment regeneration. We conclude that RDH10 is not the dominant retina 11-cis-RDH, leaving its primary function in the retina unknown.
format	article
author	Yunlu Xue Shinya Sato David Razafsky Bhubanananda Sahu Susan Q. Shen Chloe Potter Lisa L. Sandell Joseph C. Corbo Krzysztof Palczewski Akiko Maeda Didier Hodzic Vladimir J. Kefalov
author_facet	Yunlu Xue Shinya Sato David Razafsky Bhubanananda Sahu Susan Q. Shen Chloe Potter Lisa L. Sandell Joseph C. Corbo Krzysztof Palczewski Akiko Maeda Didier Hodzic Vladimir J. Kefalov
author_sort	Yunlu Xue
title	The role of retinol dehydrogenase 10 in the cone visual cycle
title_short	The role of retinol dehydrogenase 10 in the cone visual cycle
title_full	The role of retinol dehydrogenase 10 in the cone visual cycle
title_fullStr	The role of retinol dehydrogenase 10 in the cone visual cycle
title_full_unstemmed	The role of retinol dehydrogenase 10 in the cone visual cycle
title_sort	role of retinol dehydrogenase 10 in the cone visual cycle
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/392513b8e5794336a8525df1ce0d8453
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The role of retinol dehydrogenase 10 in the cone visual cycle

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