Acanthamoeba encystment: multifactorial effects of buffers, biocides, and demulcents present in contact lens care solutions

Christopher J Kovacs, Shawn C Lynch, Marjorie J Rah, Kimberly A Millard, Timothy W Morris Bausch & Lomb Incorporated, Rochester, NY, USA Purpose: To determine whether agents which are purportedly capable of inducing encystment of Acanthamoeba can recapitulate the signal when tested in diff...

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Autores principales:	Kovacs CJ, Lynch SC, Rah MJ, Millard KA, Morris TW
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2015
Materias:	Ophthalmology RE1-994
Acceso en línea:	https://doaj.org/article/39262589c8e14437978683bfe820136c
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spelling	oai:doaj.org-article:39262589c8e14437978683bfe820136c2021-12-02T06:49:12ZAcanthamoeba encystment: multifactorial effects of buffers, biocides, and demulcents present in contact lens care solutions1177-5483https://doaj.org/article/39262589c8e14437978683bfe820136c2015-10-01T00:00:00Zhttps://www.dovepress.com/acanthamoeba-encystment-multifactorial-effects-of-buffers-biocides-and-peer-reviewed-article-OPTHhttps://doaj.org/toc/1177-5483Christopher J Kovacs, Shawn C Lynch, Marjorie J Rah, Kimberly A Millard, Timothy W Morris Bausch & Lomb Incorporated, Rochester, NY, USA Purpose: To determine whether agents which are purportedly capable of inducing encystment of Acanthamoeba can recapitulate the signal when tested in differing formulations. Methods: In accordance with the International Standard ISO 19045, Acanthamoeba castellanii ATCC 50370 trophozoites were cultured in antibiotic-free axenic medium, treated with test solutions, and encystment rates plus viability were measured via bright field and fluorescent microscopy. Test solutions included phosphate-buffered saline (PBS), borate-buffered saline, biguanide- and hydrogen peroxide (H2O2)-based biocides, propylene glycol (PG) and povidone (POV) ophthalmic demulcents, and one-step H2O2-based contact lens disinfection systems. Results: Only PBS solutions with 0.25 ppm polyaminopropyl biguanide (PAPB) and increasing concentrations of PG and POV stimulated A. castellanii encystment in a dose-dependent manner, whereas PBS solutions containing 3% H2O2 and increasing concentrations of PG and POV did not stimulate encystment. Borate-buffered saline and PBS/citrate solutions containing PG also did not stimulate encystment. In addition, no encystment was observed after 24 hours, 7 days, or 14 days of exposures of trophozoites to one-step H2O2 contact lens disinfection products or related solutions. Conclusion: The lack of any encystment observed when trophozoites were treated with existing or new one-step H2O2 contact lens care products, as well as when trophozoites were exposed to various related test solutions, confirms that Acanthamoeba encystment is a complex process which depends upon simultaneous contributions of multiple factors including buffers, biocides, and demulcents. Keywords: propylene glycol, contact lens care system, hydrogen peroxide disinfecting solutionKovacs CJLynch SCRah MJMillard KAMorris TWDove Medical PressarticleOphthalmologyRE1-994ENClinical Ophthalmology, Vol 2015, Iss default, Pp 1905-1913 (2015)
institution	DOAJ
collection	DOAJ
language	EN
topic	Ophthalmology RE1-994
spellingShingle	Ophthalmology RE1-994 Kovacs CJ Lynch SC Rah MJ Millard KA Morris TW Acanthamoeba encystment: multifactorial effects of buffers, biocides, and demulcents present in contact lens care solutions
description	Christopher J Kovacs, Shawn C Lynch, Marjorie J Rah, Kimberly A Millard, Timothy W Morris Bausch & Lomb Incorporated, Rochester, NY, USA Purpose: To determine whether agents which are purportedly capable of inducing encystment of Acanthamoeba can recapitulate the signal when tested in differing formulations. Methods: In accordance with the International Standard ISO 19045, Acanthamoeba castellanii ATCC 50370 trophozoites were cultured in antibiotic-free axenic medium, treated with test solutions, and encystment rates plus viability were measured via bright field and fluorescent microscopy. Test solutions included phosphate-buffered saline (PBS), borate-buffered saline, biguanide- and hydrogen peroxide (H2O2)-based biocides, propylene glycol (PG) and povidone (POV) ophthalmic demulcents, and one-step H2O2-based contact lens disinfection systems. Results: Only PBS solutions with 0.25 ppm polyaminopropyl biguanide (PAPB) and increasing concentrations of PG and POV stimulated A. castellanii encystment in a dose-dependent manner, whereas PBS solutions containing 3% H2O2 and increasing concentrations of PG and POV did not stimulate encystment. Borate-buffered saline and PBS/citrate solutions containing PG also did not stimulate encystment. In addition, no encystment was observed after 24 hours, 7 days, or 14 days of exposures of trophozoites to one-step H2O2 contact lens disinfection products or related solutions. Conclusion: The lack of any encystment observed when trophozoites were treated with existing or new one-step H2O2 contact lens care products, as well as when trophozoites were exposed to various related test solutions, confirms that Acanthamoeba encystment is a complex process which depends upon simultaneous contributions of multiple factors including buffers, biocides, and demulcents. Keywords: propylene glycol, contact lens care system, hydrogen peroxide disinfecting solution
format	article
author	Kovacs CJ Lynch SC Rah MJ Millard KA Morris TW
author_facet	Kovacs CJ Lynch SC Rah MJ Millard KA Morris TW
author_sort	Kovacs CJ
title	Acanthamoeba encystment: multifactorial effects of buffers, biocides, and demulcents present in contact lens care solutions
title_short	Acanthamoeba encystment: multifactorial effects of buffers, biocides, and demulcents present in contact lens care solutions
title_full	Acanthamoeba encystment: multifactorial effects of buffers, biocides, and demulcents present in contact lens care solutions
title_fullStr	Acanthamoeba encystment: multifactorial effects of buffers, biocides, and demulcents present in contact lens care solutions
title_full_unstemmed	Acanthamoeba encystment: multifactorial effects of buffers, biocides, and demulcents present in contact lens care solutions
title_sort	acanthamoeba encystment: multifactorial effects of buffers, biocides, and demulcents present in contact lens care solutions
publisher	Dove Medical Press
publishDate	2015
url	https://doaj.org/article/39262589c8e14437978683bfe820136c
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Acanthamoeba encystment: multifactorial effects of buffers, biocides, and demulcents present in contact lens care solutions

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