Evaluation of nanoparticle uptake in co-culture cancer models.

Co-culture models are currently bridging the gap between classical cultures and in vivo animal models. Exploring this novel approach unlocks the possibility to mimic the tumor microenvironment in vitro, through the establishment of cancer-stroma synergistic interactions. Notably, these organotypic m...

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Autores principales:	Elisabete C Costa, Vítor M Gaspar, João G Marques, Paula Coutinho, Ilídio J Correia
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/392f61d3d8854dba8a3f1a2c42467499
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spelling	oai:doaj.org-article:392f61d3d8854dba8a3f1a2c424674992021-11-18T09:02:37ZEvaluation of nanoparticle uptake in co-culture cancer models.1932-620310.1371/journal.pone.0070072https://doaj.org/article/392f61d3d8854dba8a3f1a2c424674992013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23922909/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Co-culture models are currently bridging the gap between classical cultures and in vivo animal models. Exploring this novel approach unlocks the possibility to mimic the tumor microenvironment in vitro, through the establishment of cancer-stroma synergistic interactions. Notably, these organotypic models offer a perfect platform for the development and pre-clinical evaluation of candidate nanocarriers loaded with anti-tumoral drugs in a high throughput screening mode, with lower costs and absence of ethical issues. However, this evaluation was until now limited to co-culture systems established with precise cell ratios, not addressing the natural cell heterogeneity commonly found in different tumors. Therefore, herein the multifunctional nanocarriers efficiency was characterized in various fibroblast-MCF-7 co-culture systems containing different cell ratios, in order to unravel key design parameters that influence nanocarrier performance and the therapeutic outcome. The successful establishment of the co-culture models was confirmed by the tissue-like distribution of the different cells in culture. Nanoparticles incubation in the various co-culture systems reveals that these nanocarriers possess targeting specificity for cancer cells, indicating their suitability for being used in this illness therapy. Additionally, by using different co-culture ratios, different nanoparticle uptake profiles were obtained. These findings are of crucial importance for the future design and optimization of new drug delivery systems, since their real targeting capacity must be addressed in heterogenous cell populations, such as those found in tumors.Elisabete C CostaVítor M GasparJoão G MarquesPaula CoutinhoIlídio J CorreiaPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 7, p e70072 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Elisabete C Costa Vítor M Gaspar João G Marques Paula Coutinho Ilídio J Correia Evaluation of nanoparticle uptake in co-culture cancer models.
description	Co-culture models are currently bridging the gap between classical cultures and in vivo animal models. Exploring this novel approach unlocks the possibility to mimic the tumor microenvironment in vitro, through the establishment of cancer-stroma synergistic interactions. Notably, these organotypic models offer a perfect platform for the development and pre-clinical evaluation of candidate nanocarriers loaded with anti-tumoral drugs in a high throughput screening mode, with lower costs and absence of ethical issues. However, this evaluation was until now limited to co-culture systems established with precise cell ratios, not addressing the natural cell heterogeneity commonly found in different tumors. Therefore, herein the multifunctional nanocarriers efficiency was characterized in various fibroblast-MCF-7 co-culture systems containing different cell ratios, in order to unravel key design parameters that influence nanocarrier performance and the therapeutic outcome. The successful establishment of the co-culture models was confirmed by the tissue-like distribution of the different cells in culture. Nanoparticles incubation in the various co-culture systems reveals that these nanocarriers possess targeting specificity for cancer cells, indicating their suitability for being used in this illness therapy. Additionally, by using different co-culture ratios, different nanoparticle uptake profiles were obtained. These findings are of crucial importance for the future design and optimization of new drug delivery systems, since their real targeting capacity must be addressed in heterogenous cell populations, such as those found in tumors.
format	article
author	Elisabete C Costa Vítor M Gaspar João G Marques Paula Coutinho Ilídio J Correia
author_facet	Elisabete C Costa Vítor M Gaspar João G Marques Paula Coutinho Ilídio J Correia
author_sort	Elisabete C Costa
title	Evaluation of nanoparticle uptake in co-culture cancer models.
title_short	Evaluation of nanoparticle uptake in co-culture cancer models.
title_full	Evaluation of nanoparticle uptake in co-culture cancer models.
title_fullStr	Evaluation of nanoparticle uptake in co-culture cancer models.
title_full_unstemmed	Evaluation of nanoparticle uptake in co-culture cancer models.
title_sort	evaluation of nanoparticle uptake in co-culture cancer models.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/392f61d3d8854dba8a3f1a2c42467499
work_keys_str_mv	AT elisabeteccosta evaluationofnanoparticleuptakeincoculturecancermodels AT vitormgaspar evaluationofnanoparticleuptakeincoculturecancermodels AT joaogmarques evaluationofnanoparticleuptakeincoculturecancermodels AT paulacoutinho evaluationofnanoparticleuptakeincoculturecancermodels AT ilidiojcorreia evaluationofnanoparticleuptakeincoculturecancermodels
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Evaluation of nanoparticle uptake in co-culture cancer models.

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