Discovery of processive catalysis by an exo-hydrolase with a pocket-shaped active site

Enzyme substrates and products often diffuse too rapidly to assess the catalytic implications of these movements. Here, the authors characterise the structural basis of product and substrate diffusion for an exo-hydrolase and discover a substrate-product assisted processive catalytic mechanism.

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Autores principales:	Victor A. Streltsov, Sukanya Luang, Alys Peisley, Joseph N. Varghese, James R. Ketudat Cairns, Sebastien Fort, Marcel Hijnen, Igor Tvaroška, Ana Ardá, Jesús Jiménez-Barbero, Mercedes Alfonso-Prieto, Carme Rovira, Fernanda Mendoza, Laura Tiessler-Sala, José-Emilio Sánchez-Aparicio, Jaime Rodríguez-Guerra, José M. Lluch, Jean-Didier Maréchal, Laura Masgrau, Maria Hrmova
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2019
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/393023e627f645a0b2087690681cc2f6
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spelling	oai:doaj.org-article:393023e627f645a0b2087690681cc2f62021-12-02T16:58:06ZDiscovery of processive catalysis by an exo-hydrolase with a pocket-shaped active site10.1038/s41467-019-09691-z2041-1723https://doaj.org/article/393023e627f645a0b2087690681cc2f62019-05-01T00:00:00Zhttps://doi.org/10.1038/s41467-019-09691-zhttps://doaj.org/toc/2041-1723Enzyme substrates and products often diffuse too rapidly to assess the catalytic implications of these movements. Here, the authors characterise the structural basis of product and substrate diffusion for an exo-hydrolase and discover a substrate-product assisted processive catalytic mechanism.Victor A. StreltsovSukanya LuangAlys PeisleyJoseph N. VargheseJames R. Ketudat CairnsSebastien FortMarcel HijnenIgor TvaroškaAna ArdáJesús Jiménez-BarberoMercedes Alfonso-PrietoCarme RoviraFernanda MendozaLaura Tiessler-SalaJosé-Emilio Sánchez-AparicioJaime Rodríguez-GuerraJosé M. LluchJean-Didier MaréchalLaura MasgrauMaria HrmovaNature PortfolioarticleScienceQENNature Communications, Vol 10, Iss 1, Pp 1-17 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Victor A. Streltsov Sukanya Luang Alys Peisley Joseph N. Varghese James R. Ketudat Cairns Sebastien Fort Marcel Hijnen Igor Tvaroška Ana Ardá Jesús Jiménez-Barbero Mercedes Alfonso-Prieto Carme Rovira Fernanda Mendoza Laura Tiessler-Sala José-Emilio Sánchez-Aparicio Jaime Rodríguez-Guerra José M. Lluch Jean-Didier Maréchal Laura Masgrau Maria Hrmova Discovery of processive catalysis by an exo-hydrolase with a pocket-shaped active site
description	Enzyme substrates and products often diffuse too rapidly to assess the catalytic implications of these movements. Here, the authors characterise the structural basis of product and substrate diffusion for an exo-hydrolase and discover a substrate-product assisted processive catalytic mechanism.
format	article
author	Victor A. Streltsov Sukanya Luang Alys Peisley Joseph N. Varghese James R. Ketudat Cairns Sebastien Fort Marcel Hijnen Igor Tvaroška Ana Ardá Jesús Jiménez-Barbero Mercedes Alfonso-Prieto Carme Rovira Fernanda Mendoza Laura Tiessler-Sala José-Emilio Sánchez-Aparicio Jaime Rodríguez-Guerra José M. Lluch Jean-Didier Maréchal Laura Masgrau Maria Hrmova
author_facet	Victor A. Streltsov Sukanya Luang Alys Peisley Joseph N. Varghese James R. Ketudat Cairns Sebastien Fort Marcel Hijnen Igor Tvaroška Ana Ardá Jesús Jiménez-Barbero Mercedes Alfonso-Prieto Carme Rovira Fernanda Mendoza Laura Tiessler-Sala José-Emilio Sánchez-Aparicio Jaime Rodríguez-Guerra José M. Lluch Jean-Didier Maréchal Laura Masgrau Maria Hrmova
author_sort	Victor A. Streltsov
title	Discovery of processive catalysis by an exo-hydrolase with a pocket-shaped active site
title_short	Discovery of processive catalysis by an exo-hydrolase with a pocket-shaped active site
title_full	Discovery of processive catalysis by an exo-hydrolase with a pocket-shaped active site
title_fullStr	Discovery of processive catalysis by an exo-hydrolase with a pocket-shaped active site
title_full_unstemmed	Discovery of processive catalysis by an exo-hydrolase with a pocket-shaped active site
title_sort	discovery of processive catalysis by an exo-hydrolase with a pocket-shaped active site
publisher	Nature Portfolio
publishDate	2019
url	https://doaj.org/article/393023e627f645a0b2087690681cc2f6
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Discovery of processive catalysis by an exo-hydrolase with a pocket-shaped active site

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