Changes in the Expression of Renal Brush Border Membrane <i>N</i>-Glycome in Model Rats with Chronic Kidney Diseases

Chronic kidney disease (CKD) is defined by a reduced renal function i.e., glomerular filtration rate (GFR), and the presence of kidney damage is determined by measurement of proteinuria or albuminuria. Albuminuria increases with age and can result from glomerular and/or proximal tubule (PT) alterati...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Aiying Yu, Jingfu Zhao, Shiv Pratap S. Yadav, Bruce A. Molitoris, Mark C. Wagner, Yehia Mechref
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	glycomics brush-border membrane chronic kidney disease proteinuria and hypertension obese and diabetic LC-MS/MS Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/393b464682b64cd38ddfd977ec159c62
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:393b464682b64cd38ddfd977ec159c62
record_format	dspace
spelling	oai:doaj.org-article:393b464682b64cd38ddfd977ec159c622021-11-25T16:53:43ZChanges in the Expression of Renal Brush Border Membrane <i>N</i>-Glycome in Model Rats with Chronic Kidney Diseases10.3390/biom111116772218-273Xhttps://doaj.org/article/393b464682b64cd38ddfd977ec159c622021-11-01T00:00:00Zhttps://www.mdpi.com/2218-273X/11/11/1677https://doaj.org/toc/2218-273XChronic kidney disease (CKD) is defined by a reduced renal function i.e., glomerular filtration rate (GFR), and the presence of kidney damage is determined by measurement of proteinuria or albuminuria. Albuminuria increases with age and can result from glomerular and/or proximal tubule (PT) alterations. Brush-border membranes (BBMs) on PT cells play an important role in maintaining the stability of PT functions. The PT BBM, a highly dynamic, organized, specialized membrane, contains a variety of glycoproteins required for the functions of PT. Since protein glycosylation regulates many protein functions, the alteration of glycosylation due to the glycan changes has attracted more interests for a variety of disease studies recently. In this work, liquid chromatography-tandem mass spectrometry was utilized to analyze the abundances of permethylated glycans from rats under control to mild CKD, severe CKD, and diabetic conditions. The most significant differences were observed in sialylation level with the highest present in the severe CKD and diabetic groups. Moreover, high mannose <i>N</i>-glycans was enriched in the CKD BBMs. Characterization of all the BBM <i>N</i>-glycan changes supports that these changes are likely to impact the functional properties of the dynamic PT BBM. Further, these changes may lead to the potential discovery of glycan biomarkers for improved CKD diagnosis and new avenues for therapeutic treatments.Aiying YuJingfu ZhaoShiv Pratap S. YadavBruce A. MolitorisMark C. WagnerYehia MechrefMDPI AGarticleglycomicsbrush-border membranechronic kidney diseaseproteinuria and hypertensionobese and diabeticLC-MS/MSMicrobiologyQR1-502ENBiomolecules, Vol 11, Iss 1677, p 1677 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	glycomics brush-border membrane chronic kidney disease proteinuria and hypertension obese and diabetic LC-MS/MS Microbiology QR1-502
spellingShingle	glycomics brush-border membrane chronic kidney disease proteinuria and hypertension obese and diabetic LC-MS/MS Microbiology QR1-502 Aiying Yu Jingfu Zhao Shiv Pratap S. Yadav Bruce A. Molitoris Mark C. Wagner Yehia Mechref Changes in the Expression of Renal Brush Border Membrane <i>N</i>-Glycome in Model Rats with Chronic Kidney Diseases
description	Chronic kidney disease (CKD) is defined by a reduced renal function i.e., glomerular filtration rate (GFR), and the presence of kidney damage is determined by measurement of proteinuria or albuminuria. Albuminuria increases with age and can result from glomerular and/or proximal tubule (PT) alterations. Brush-border membranes (BBMs) on PT cells play an important role in maintaining the stability of PT functions. The PT BBM, a highly dynamic, organized, specialized membrane, contains a variety of glycoproteins required for the functions of PT. Since protein glycosylation regulates many protein functions, the alteration of glycosylation due to the glycan changes has attracted more interests for a variety of disease studies recently. In this work, liquid chromatography-tandem mass spectrometry was utilized to analyze the abundances of permethylated glycans from rats under control to mild CKD, severe CKD, and diabetic conditions. The most significant differences were observed in sialylation level with the highest present in the severe CKD and diabetic groups. Moreover, high mannose <i>N</i>-glycans was enriched in the CKD BBMs. Characterization of all the BBM <i>N</i>-glycan changes supports that these changes are likely to impact the functional properties of the dynamic PT BBM. Further, these changes may lead to the potential discovery of glycan biomarkers for improved CKD diagnosis and new avenues for therapeutic treatments.
format	article
author	Aiying Yu Jingfu Zhao Shiv Pratap S. Yadav Bruce A. Molitoris Mark C. Wagner Yehia Mechref
author_facet	Aiying Yu Jingfu Zhao Shiv Pratap S. Yadav Bruce A. Molitoris Mark C. Wagner Yehia Mechref
author_sort	Aiying Yu
title	Changes in the Expression of Renal Brush Border Membrane <i>N</i>-Glycome in Model Rats with Chronic Kidney Diseases
title_short	Changes in the Expression of Renal Brush Border Membrane <i>N</i>-Glycome in Model Rats with Chronic Kidney Diseases
title_full	Changes in the Expression of Renal Brush Border Membrane <i>N</i>-Glycome in Model Rats with Chronic Kidney Diseases
title_fullStr	Changes in the Expression of Renal Brush Border Membrane <i>N</i>-Glycome in Model Rats with Chronic Kidney Diseases
title_full_unstemmed	Changes in the Expression of Renal Brush Border Membrane <i>N</i>-Glycome in Model Rats with Chronic Kidney Diseases
title_sort	changes in the expression of renal brush border membrane <i>n</i>-glycome in model rats with chronic kidney diseases
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/393b464682b64cd38ddfd977ec159c62
work_keys_str_mv	AT aiyingyu changesintheexpressionofrenalbrushbordermembraneiniglycomeinmodelratswithchronickidneydiseases AT jingfuzhao changesintheexpressionofrenalbrushbordermembraneiniglycomeinmodelratswithchronickidneydiseases AT shivpratapsyadav changesintheexpressionofrenalbrushbordermembraneiniglycomeinmodelratswithchronickidneydiseases AT bruceamolitoris changesintheexpressionofrenalbrushbordermembraneiniglycomeinmodelratswithchronickidneydiseases AT markcwagner changesintheexpressionofrenalbrushbordermembraneiniglycomeinmodelratswithchronickidneydiseases AT yehiamechref changesintheexpressionofrenalbrushbordermembraneiniglycomeinmodelratswithchronickidneydiseases
_version_	1718412887254368256

Changes in the Expression of Renal Brush Border Membrane <i>N</i>-Glycome in Model Rats with Chronic Kidney Diseases

Ejemplares similares