In Vitro Red Blood Cell Segregation in Sickle Cell Anemia

Red blood cells in sickle cell anemia (sRBC) are more heterogeneous in their physical properties than healthy red blood cells, spanning adhesiveness, rigidity, density, size, and shape. sRBC with increased adhesiveness to the vascular wall would trigger vaso-occlusive like complications, a hallmark...

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Autores principales:	Viviana Clavería, Philippe Connes, Luca Lanotte, Céline Renoux, Philippe Joly, Romain Fort, Alexandra Gauthier, Christian Wagner, Manouk Abkarian
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	sickle cell anemia red blood cells segregation red blood cell density microfluidics Physics QC1-999
Acceso en línea:	https://doaj.org/article/3940873705f7411a8008eda9a73fe28b
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spelling	oai:doaj.org-article:3940873705f7411a8008eda9a73fe28b2021-12-02T10:16:22ZIn Vitro Red Blood Cell Segregation in Sickle Cell Anemia2296-424X10.3389/fphy.2021.737739https://doaj.org/article/3940873705f7411a8008eda9a73fe28b2021-12-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fphy.2021.737739/fullhttps://doaj.org/toc/2296-424XRed blood cells in sickle cell anemia (sRBC) are more heterogeneous in their physical properties than healthy red blood cells, spanning adhesiveness, rigidity, density, size, and shape. sRBC with increased adhesiveness to the vascular wall would trigger vaso-occlusive like complications, a hallmark of sickle cell anemia. We investigated whether segregation occurs among sRBC flowing in micron-sized channels and tested the impact of aggregation on segregation. Two populations of sRBC of different densities were separated, labeled, and mixed again. The mixed suspension was flowed within glass capillary tubes at different pressure-drops, hematocrit, and suspending media that promoted or not cell aggregation. Observations were made at a fixed channel position. The mean flow velocity was obtained by using the cells as tracking particles, and the cell depleted layer (CDL) by measuring the distance from the cell core border to the channel wall. The labeled sRBC were identified by stopping the flow and scanning the cells within the channel section. The tube hematocrit was estimated from the number of fluorescence cells identified in the field of view. In non-aggregating media, our results showed a heterogeneous distribution of sRBC according to their density: low-density sRBC population remained closer to the center of the channel, while the densest cells segregated towards the walls. There was no impact of the mean flow velocity and little impact of hematocrit. This segregation heterogeneity could influence the ability of sRBC to adhere to the vascular wall and slow down blood flow. However, promoting aggregation inhibited segregation while CDL thickness was enhanced by aggregation, highlighting a potential protective role against vaso-occlusion in patients with sickle cell anemia.Viviana ClaveríaViviana ClaveríaPhilippe ConnesPhilippe ConnesPhilippe ConnesLuca LanotteCéline RenouxCéline RenouxCéline RenouxPhilippe JolyPhilippe JolyPhilippe JolyRomain FortRomain FortRomain FortAlexandra GauthierAlexandra GauthierAlexandra GauthierChristian WagnerManouk AbkarianManouk AbkarianFrontiers Media S.A.articlesickle cell anemiared blood cellssegregationred blood cell densitymicrofluidicsPhysicsQC1-999ENFrontiers in Physics, Vol 9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	sickle cell anemia red blood cells segregation red blood cell density microfluidics Physics QC1-999
spellingShingle	sickle cell anemia red blood cells segregation red blood cell density microfluidics Physics QC1-999 Viviana Clavería Viviana Clavería Philippe Connes Philippe Connes Philippe Connes Luca Lanotte Céline Renoux Céline Renoux Céline Renoux Philippe Joly Philippe Joly Philippe Joly Romain Fort Romain Fort Romain Fort Alexandra Gauthier Alexandra Gauthier Alexandra Gauthier Christian Wagner Manouk Abkarian Manouk Abkarian In Vitro Red Blood Cell Segregation in Sickle Cell Anemia
description	Red blood cells in sickle cell anemia (sRBC) are more heterogeneous in their physical properties than healthy red blood cells, spanning adhesiveness, rigidity, density, size, and shape. sRBC with increased adhesiveness to the vascular wall would trigger vaso-occlusive like complications, a hallmark of sickle cell anemia. We investigated whether segregation occurs among sRBC flowing in micron-sized channels and tested the impact of aggregation on segregation. Two populations of sRBC of different densities were separated, labeled, and mixed again. The mixed suspension was flowed within glass capillary tubes at different pressure-drops, hematocrit, and suspending media that promoted or not cell aggregation. Observations were made at a fixed channel position. The mean flow velocity was obtained by using the cells as tracking particles, and the cell depleted layer (CDL) by measuring the distance from the cell core border to the channel wall. The labeled sRBC were identified by stopping the flow and scanning the cells within the channel section. The tube hematocrit was estimated from the number of fluorescence cells identified in the field of view. In non-aggregating media, our results showed a heterogeneous distribution of sRBC according to their density: low-density sRBC population remained closer to the center of the channel, while the densest cells segregated towards the walls. There was no impact of the mean flow velocity and little impact of hematocrit. This segregation heterogeneity could influence the ability of sRBC to adhere to the vascular wall and slow down blood flow. However, promoting aggregation inhibited segregation while CDL thickness was enhanced by aggregation, highlighting a potential protective role against vaso-occlusion in patients with sickle cell anemia.
format	article
author	Viviana Clavería Viviana Clavería Philippe Connes Philippe Connes Philippe Connes Luca Lanotte Céline Renoux Céline Renoux Céline Renoux Philippe Joly Philippe Joly Philippe Joly Romain Fort Romain Fort Romain Fort Alexandra Gauthier Alexandra Gauthier Alexandra Gauthier Christian Wagner Manouk Abkarian Manouk Abkarian
author_facet	Viviana Clavería Viviana Clavería Philippe Connes Philippe Connes Philippe Connes Luca Lanotte Céline Renoux Céline Renoux Céline Renoux Philippe Joly Philippe Joly Philippe Joly Romain Fort Romain Fort Romain Fort Alexandra Gauthier Alexandra Gauthier Alexandra Gauthier Christian Wagner Manouk Abkarian Manouk Abkarian
author_sort	Viviana Clavería
title	In Vitro Red Blood Cell Segregation in Sickle Cell Anemia
title_short	In Vitro Red Blood Cell Segregation in Sickle Cell Anemia
title_full	In Vitro Red Blood Cell Segregation in Sickle Cell Anemia
title_fullStr	In Vitro Red Blood Cell Segregation in Sickle Cell Anemia
title_full_unstemmed	In Vitro Red Blood Cell Segregation in Sickle Cell Anemia
title_sort	in vitro red blood cell segregation in sickle cell anemia
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/3940873705f7411a8008eda9a73fe28b
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In Vitro Red Blood Cell Segregation in Sickle Cell Anemia

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