Uvaol Prevents Group B Streptococcus-Induced Trophoblast Cells Inflammation and Possible Endothelial Dysfunction

Group B Streptococcus (GBS) infection during pregnancy is involved in maternal sepsis, chorioamnionitis, prematurity, fetal infection, neonatal sepsis, and neurodevelopmental alterations. The GBS-induced chorioamnionitis leads to a plethora of immune and trophoblast cells alterations that could infl...

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Autores principales:	Ana Lucia Mendes Silva, Elaine Cristina Oliveira Silva, Rayane Martins Botelho, Liliane Patricia Gonçalves Tenorio, Aldilane Lays Xavier Marques, Ingredy Brunele Albuquerque Costa Rodrigues, Larissa Iolanda Moreira Almeida, Ashelley Kettyllem Alves Sousa, Keyla Silva Nobre Pires, Ithallo Sathio Bessoni Tanabe, Marie-Julie Allard, Guillaume Sébire, Samuel Teixeira Souza, Eduardo Jorge Silva Fonseca, Karen Steponavicius Cruz Borbely, Alexandre Urban Borbely
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	Group B Streptococcus infection placenta vascular dysfunction trophoblast uvaol Physiology QP1-981
Acceso en línea:	https://doaj.org/article/3940a0c014814b19be503592434e271f
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spelling	oai:doaj.org-article:3940a0c014814b19be503592434e271f2021-12-03T06:17:08ZUvaol Prevents Group B Streptococcus-Induced Trophoblast Cells Inflammation and Possible Endothelial Dysfunction1664-042X10.3389/fphys.2021.766382https://doaj.org/article/3940a0c014814b19be503592434e271f2021-12-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fphys.2021.766382/fullhttps://doaj.org/toc/1664-042XGroup B Streptococcus (GBS) infection during pregnancy is involved in maternal sepsis, chorioamnionitis, prematurity, fetal infection, neonatal sepsis, and neurodevelopmental alterations. The GBS-induced chorioamnionitis leads to a plethora of immune and trophoblast cells alterations that could influence endothelial cells to respond differently to angiogenic mediators and alter placental vascular structure and function in pregnant women. In this context, preventive measures are needed to reduce such dysfunctions. As such, we evaluated the effects of a non-lethal exposure to inactivated GBS on trophoblast cells and chorionic villi explants, and if the treatment with uvaol would mitigate these effects. The concentration of 106 CFU of GBS was chosen since it was unable to reduce the HTR-8/SVneo cell line nor term chorionic villi explant viability. Raman spectroscopy of trophoblast cells showed significant alterations in their biochemical signature, mostly reverted by uvaol. GBS exposure increased HTR-8/SVneo cells IL-1β and IFN-γ production, phagocytosis, oxidative stress, and decreased trophoblast cell migration. The Ea.hy926 endothelial cell line produced angiopoietin-2, CXCL-8, EGF, FGF-b, IL-6, PlGF, sPECAM-1, and VEGF in culture. When co-cultured in invasion assay with HTR-8/SVneo trophoblast cells, the co-culture had increased production of angiopoietin-2, CXCL-8, FGF-b, and VEGF, while reduced sPECAM-1 and IL-6. GBS exposure led to increased CXCL-8 and IL-6 production, both prevented by uvaol. Chorionic villi explants followed the same patterns of production when exposed to GBS and response to uvaol treatment as well. These findings demonstrate that, even a non-lethal concentration of GBS causes placental inflammation and oxidative stress, reduces trophoblast invasion of endothelial cells, and increases CXCL-8 and IL-6, key factors that participate in vascular dysregulation observed in several diseases. Furthermore, uvaol treatment prevented most of the GBS-provoked changes. Hence, uvaol could prevent the harmful effects of GBS infection for both the mother and the fetus.Ana Lucia Mendes SilvaElaine Cristina Oliveira SilvaRayane Martins BotelhoLiliane Patricia Gonçalves TenorioAldilane Lays Xavier MarquesIngredy Brunele Albuquerque Costa RodriguesLarissa Iolanda Moreira AlmeidaAshelley Kettyllem Alves SousaKeyla Silva Nobre PiresIthallo Sathio Bessoni TanabeMarie-Julie AllardGuillaume SébireGuillaume SébireSamuel Teixeira SouzaEduardo Jorge Silva FonsecaKaren Steponavicius Cruz BorbelyKaren Steponavicius Cruz BorbelyAlexandre Urban BorbelyFrontiers Media S.A.articleGroup B Streptococcusinfectionplacentavascular dysfunctiontrophoblastuvaolPhysiologyQP1-981ENFrontiers in Physiology, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Group B Streptococcus infection placenta vascular dysfunction trophoblast uvaol Physiology QP1-981
spellingShingle	Group B Streptococcus infection placenta vascular dysfunction trophoblast uvaol Physiology QP1-981 Ana Lucia Mendes Silva Elaine Cristina Oliveira Silva Rayane Martins Botelho Liliane Patricia Gonçalves Tenorio Aldilane Lays Xavier Marques Ingredy Brunele Albuquerque Costa Rodrigues Larissa Iolanda Moreira Almeida Ashelley Kettyllem Alves Sousa Keyla Silva Nobre Pires Ithallo Sathio Bessoni Tanabe Marie-Julie Allard Guillaume Sébire Guillaume Sébire Samuel Teixeira Souza Eduardo Jorge Silva Fonseca Karen Steponavicius Cruz Borbely Karen Steponavicius Cruz Borbely Alexandre Urban Borbely Uvaol Prevents Group B Streptococcus-Induced Trophoblast Cells Inflammation and Possible Endothelial Dysfunction
description	Group B Streptococcus (GBS) infection during pregnancy is involved in maternal sepsis, chorioamnionitis, prematurity, fetal infection, neonatal sepsis, and neurodevelopmental alterations. The GBS-induced chorioamnionitis leads to a plethora of immune and trophoblast cells alterations that could influence endothelial cells to respond differently to angiogenic mediators and alter placental vascular structure and function in pregnant women. In this context, preventive measures are needed to reduce such dysfunctions. As such, we evaluated the effects of a non-lethal exposure to inactivated GBS on trophoblast cells and chorionic villi explants, and if the treatment with uvaol would mitigate these effects. The concentration of 106 CFU of GBS was chosen since it was unable to reduce the HTR-8/SVneo cell line nor term chorionic villi explant viability. Raman spectroscopy of trophoblast cells showed significant alterations in their biochemical signature, mostly reverted by uvaol. GBS exposure increased HTR-8/SVneo cells IL-1β and IFN-γ production, phagocytosis, oxidative stress, and decreased trophoblast cell migration. The Ea.hy926 endothelial cell line produced angiopoietin-2, CXCL-8, EGF, FGF-b, IL-6, PlGF, sPECAM-1, and VEGF in culture. When co-cultured in invasion assay with HTR-8/SVneo trophoblast cells, the co-culture had increased production of angiopoietin-2, CXCL-8, FGF-b, and VEGF, while reduced sPECAM-1 and IL-6. GBS exposure led to increased CXCL-8 and IL-6 production, both prevented by uvaol. Chorionic villi explants followed the same patterns of production when exposed to GBS and response to uvaol treatment as well. These findings demonstrate that, even a non-lethal concentration of GBS causes placental inflammation and oxidative stress, reduces trophoblast invasion of endothelial cells, and increases CXCL-8 and IL-6, key factors that participate in vascular dysregulation observed in several diseases. Furthermore, uvaol treatment prevented most of the GBS-provoked changes. Hence, uvaol could prevent the harmful effects of GBS infection for both the mother and the fetus.
format	article
author	Ana Lucia Mendes Silva Elaine Cristina Oliveira Silva Rayane Martins Botelho Liliane Patricia Gonçalves Tenorio Aldilane Lays Xavier Marques Ingredy Brunele Albuquerque Costa Rodrigues Larissa Iolanda Moreira Almeida Ashelley Kettyllem Alves Sousa Keyla Silva Nobre Pires Ithallo Sathio Bessoni Tanabe Marie-Julie Allard Guillaume Sébire Guillaume Sébire Samuel Teixeira Souza Eduardo Jorge Silva Fonseca Karen Steponavicius Cruz Borbely Karen Steponavicius Cruz Borbely Alexandre Urban Borbely
author_facet	Ana Lucia Mendes Silva Elaine Cristina Oliveira Silva Rayane Martins Botelho Liliane Patricia Gonçalves Tenorio Aldilane Lays Xavier Marques Ingredy Brunele Albuquerque Costa Rodrigues Larissa Iolanda Moreira Almeida Ashelley Kettyllem Alves Sousa Keyla Silva Nobre Pires Ithallo Sathio Bessoni Tanabe Marie-Julie Allard Guillaume Sébire Guillaume Sébire Samuel Teixeira Souza Eduardo Jorge Silva Fonseca Karen Steponavicius Cruz Borbely Karen Steponavicius Cruz Borbely Alexandre Urban Borbely
author_sort	Ana Lucia Mendes Silva
title	Uvaol Prevents Group B Streptococcus-Induced Trophoblast Cells Inflammation and Possible Endothelial Dysfunction
title_short	Uvaol Prevents Group B Streptococcus-Induced Trophoblast Cells Inflammation and Possible Endothelial Dysfunction
title_full	Uvaol Prevents Group B Streptococcus-Induced Trophoblast Cells Inflammation and Possible Endothelial Dysfunction
title_fullStr	Uvaol Prevents Group B Streptococcus-Induced Trophoblast Cells Inflammation and Possible Endothelial Dysfunction
title_full_unstemmed	Uvaol Prevents Group B Streptococcus-Induced Trophoblast Cells Inflammation and Possible Endothelial Dysfunction
title_sort	uvaol prevents group b streptococcus-induced trophoblast cells inflammation and possible endothelial dysfunction
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/3940a0c014814b19be503592434e271f
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Uvaol Prevents Group B Streptococcus-Induced Trophoblast Cells Inflammation and Possible Endothelial Dysfunction

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